BGC0000964 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000964 (Burkholderia thailandensis E264)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	NRPS	1	38,578	burkholdac A/burkholdac B	NRPS:Type I+PKS:Trans-AT type I	100%

BGC0000964 - Region 1 - NRPS

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Location: 1 - 38,578 nt. (total: 38,578 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 38,579
Label		BGC0000964

Detailed domain annotation

Selected features only

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Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000964	1.00	NRP, Polyketide	burkholdac A, burkholdac B	Burkholderia thailandensis E264
BGC0001045	0.37	NRP, Polyketide	spiruchostatin A	Pseudomonas sp. Q71576
BGC0000016	0.34	Other	amphotericin B	Streptomyces nodosus
BGC0002017	0.33	NRP	potensibactin	Nocardiopsis potens DSM 45234
BGC0000993	0.32	NRP, Polyketide	FR901228	Chromobacterium violaceum
BGC0002135	0.32	NRP	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0002474	0.30	NRP	agrobactin	Agrobacterium tumefaciens
BGC0002518	0.30	NRP	syringafactin A, syringafactin C	Pseudomonas sp. SZ57
BGC0001825	0.29	NRP	xenematide	Xenorhabdus nematophila AN6/1
BGC0002287	0.29	NRP	mutanocyclin, leuvalin, tyrvalin	Streptococcus mutans

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000964	1.00	NRP, Polyketide	burkholdac A, burkholdac B	Burkholderia thailandensis E264
BGC0001045	0.69	NRP, Polyketide	spiruchostatin A	Pseudomonas sp. Q71576
BGC0000016	0.67	Other	amphotericin B	Streptomyces nodosus
BGC0002017	0.67	NRP	potensibactin	Nocardiopsis potens DSM 45234
BGC0000993	0.66	NRP, Polyketide	FR901228	Chromobacterium violaceum
BGC0002135	0.65	NRP	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0002474	0.64	NRP	agrobactin	Agrobacterium tumefaciens
BGC0002518	0.64	NRP	syringafactin A, syringafactin C	Pseudomonas sp. SZ57
BGC0001825	0.63	NRP	xenematide	Xenorhabdus nematophila AN6/1
BGC0002287	0.62	NRP	mutanocyclin, leuvalin, tyrvalin	Streptococcus mutans

Similar known gene clusters from MIBiG 4.0

Module view

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 38578:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Cys) + (Met - D-Cys - Ile)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

BTH_I2364

Search NORINE for peptide: strict or relaxed

AMP-binding (525..928): Met

nrpys: Met

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: DVFYLGGVFK
Nearest Stachelhaus code(s):: Met DVFYLGGVFK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1608..2002): Cys

nrpys: Cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: DLFEMSLIWK
Nearest Stachelhaus code(s):: Cys DLFEMSLIWK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (3059..3443): Ile

nrpys: Ile

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DYLCVGVVIK
Nearest Stachelhaus code(s):: Ile DYLCVGVVIK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

BTH_I2367

Search NORINE for peptide: strict or relaxed

CAL_domain (166..484): pk

minowa: NH2

Prediction, score:

NH2: 132.3
Acetyl-CoA: 44.8
fatty_acid: 16.2
shikimic_acid: 0.0
AHBA: -2.3

AMP-binding (1105..1490): Cys

nrpys: Cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: DLYNLSLIWK
Nearest Stachelhaus code(s):: Cys DLYNLSLIWK (100% 8Å match)
Stachelhaus code match:: 100% (strong)