BGC0000966 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000966 (Actinoalloteichus sp. WH1-2216-6)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	NRPS,T1PKS	1	44,640	High	caerulomycin A	NRPS:Type I+PKS

BGC0000966 - Region 1 - NRPS,T1PKS

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Location: 1 - 44,640 nt. (total: 44,640 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

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TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 44,641
Label		BGC0000966

Tailoring functions

Oxidoreductases: dehydrogenase oxidase oxidoreductase

AFD30946.1: FAD linked oxidase domain protein

smcogs: SMCOG1138: FAD linked oxidase domain protein

AFD30952.1: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

AFD30955.1: FAD dependent oxidoreductase

smcogs: SMCOG1103: FAD dependent oxidoreductase

AFD30961.1: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

Transferases: aminotransferase methyltransferase

AFD30956.1: DegT/DnrJ/EryC1/StrS aminotransferase

smcogs: SMCOG1056: DegT/DnrJ/EryC1/StrS aminotransferase

AFD30959.1: aminotransferase class-III

smcogs: SMCOG1013: aminotransferase class-III

AFD30960.1: O-methyltransferase

smcogs: SMCOG1042: O-methyltransferase

AFD30966.1: methyltransferase

smcogs: SMCOG1089: methyltransferase

Hydrolases: esterase

AFD30951.1: thioesterase

smcogs: SMCOG1004: thioesterase

Ligases: ligase synthase

AEM06018.2: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

AFD30953.1: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

AFD30954.1: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

AFD30957.1: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000966.5	1.97	NRPS, PKS	caerulomycin A	Actinoalloteichus sp. WH1-2216-6
BGC0000342.4	0.70	NRPS	enniatin	Fusarium equiseti
BGC0002135.2	0.68	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001833.4	0.68	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001132.4	0.67	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002286.3	0.67	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001825.4	0.66	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001261.3	0.66	NRPS	AM-toxin	Alternaria alternata
BGC0001128.4	0.66	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0000107.3	0.66	PKS	naphthopyrone	Aspergillus nidulans FGSC A4

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000966.5	1.00	NRPS, PKS	caerulomycin A	Actinoalloteichus sp. WH1-2216-6
BGC0000342.4	0.68	NRPS	enniatin	Fusarium equiseti
BGC0002135.2	0.67	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001833.4	0.67	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001132.4	0.67	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002286.3	0.67	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001825.4	0.66	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001261.3	0.66	NRPS	AM-toxin	Alternaria alternata
BGC0001128.4	0.66	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0000107.3	0.66	PKS	naphthopyrone	Aspergillus nidulans FGSC A4

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 3209 - 44640:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal - Cys) + (Leu)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

AEM06018.2: diOH-Bz

Search NORINE for peptide: strict or relaxed

AMP-binding (32..442): diOH-Bz

nrpys: diOH-Bz

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Small clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Single AA prediction:: dhb (diOH-Bz)

Stachelhaus prediction details:

Stachelhaus sequence:: VLPTQGCLCK
Nearest Stachelhaus code(s):: diOH-Bz PLPAQGVLCK (62% 8Å match); COOH-Qui ALPTQGWLTK (56% 8Å match)
Stachelhaus code match:: 70% (weak)

AFD30957.1: diOH-Bz

Search NORINE for peptide: strict or relaxed

AMP-binding (142..552): diOH-Bz

nrpys: diOH-Bz

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Small clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Single AA prediction:: dhb (diOH-Bz)

Stachelhaus prediction details:

Stachelhaus sequence:: VLPTQGCLCK
Nearest Stachelhaus code(s):: diOH-Bz PLPAQGVLCK (62% 8Å match); COOH-Qui ALPTQGWLTK (56% 8Å match)
Stachelhaus code match:: 70% (weak)

AFD30954.1: mal - Cys

Search NORINE for peptide: strict or relaxed

PKS_AT (541..839): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 75.0%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 149.9
inactive: 88.8
Methoxymalonyl-CoA: 72.3
Methylmalonyl-CoA: 61.5
Propionyl-CoA: 41.2
2-Methylbutyryl-CoA: 33.1
Isobutyryl-CoA: 24.0
Ethylmalonyl-CoA: 23.4
CHC-CoA: 21.9
fatty_acid: 21.8
trans-1,2-CPDA: 15.9
Benzoyl-CoA: 14.8
Acetyl-CoA: 12.5
3-Methylbutyryl-CoA: 0.0

AMP-binding (1502..1895): Cys

nrpys: Cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: DLFNMSLIWK
Nearest Stachelhaus code(s):: Cys DLFNMSLIWK (85% 8Å match)
Stachelhaus code match:: 100% (strong)

AFD30953.1: Leu

Search NORINE for peptide: strict or relaxed

AMP-binding (468..872): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: DYWSFGLVVK
Nearest Stachelhaus code(s):: Leu DYWSFGLVVK (85% 8Å match)
Stachelhaus code match:: 100% (strong)

AFD30948.1: X

AMP-binding (99..319): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DPAATSIIFK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

Link to PARAS substrate predictor