BGC0000967: calyculin A biosynthetic gene cluster from uncultured Candidatus Entotheonella sp.
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Location: 1 - 148,229 nt. (total: 148,229 nt).
This entry is originally from NCBI GenBank AB933566.1.

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core biosynthetic genes
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Gene details
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General
Compounds
Genes
Polyketide
NRP
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0000967
Short description calyculin A biosynthetic gene cluster from uncultured Candidatus Entotheonella sp.
Status Minimal annotation: no
A minimal annotation only contains information on the BGC loci and one or more linked chemical product(s)

Completeness: complete
Whether the loci encodes everything needed for the pathway producing the compound(s)
Biosynthetic class(es)
  • NRP
  • Polyketide (Other)
Loci NCBI GenBank: AB933566.1
Compounds
  • calyculin A
  • phosphocalyculin A
  • calyculinamide A
  • dephosphonocalyculin A
Species uncultured Candidatus Entotheonella sp. [taxonomy]
References
Chemical products information
calyculin A
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C50H81N4O15P1
Chemical database entries
PubCHEM
phosphocalyculin A
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C50H82N4O18P2
calyculinamide A
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C50H83N4O16P1
Chemical database entries
PubCHEM
ChemSpider
dephosphonocalyculin A
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C50H80N4O12
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • BAP05573.1
  • calY
1 - 2364 (+) calY
  • Scaffold biosynthesis
Sequence-based prediction
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  • BAP05574.1
  • calX
2395 - 2643 (+) calX
  • Scaffold biosynthesis
Sequence-based prediction
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  • BAP05575.1
  • calW
2621 - 3850 (+) calW
  • Scaffold biosynthesis
Sequence-based prediction
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  • BAP05576.1
  • calV
4030 - 4428 (+) calV
  • Unknown
Sequence-based prediction
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  • BAP05577.1
  • calU
4425 - 6545 (+) calU
  • Transport
Sequence-based prediction
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  • BAP05578.1
  • calT
6647 - 7921 (+) calT
  • Scaffold biosynthesis
Sequence-based prediction
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  • BAP05579.1
  • calS
7909 - 8715 (+) calS
  • Scaffold biosynthesis
Sequence-based prediction
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  • BAP05580.1
  • calR
8708 - 9445 (+) calR
  • Scaffold biosynthesis
Sequence-based prediction
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  • BAP05581.1
  • calQ
9473 - 10606 (+) calQ
  • Tailoring (Phosphorylation)
Activity assay
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  • BAP05582.1
  • calP
10603 - 11298 (+) calP
  • Tailoring (Phosphorylation)
Sequence-based prediction
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  • BAP05583.1
  • calO
11313 - 12122 (-) calO
  • Resistance/immunity
Sequence-based prediction
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  • BAP05584.1
  • calN
12379 - 13650 (-) calN
  • Unknown
Sequence-based prediction
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  • BAP05585.1
  • calM
14351 - 15049 (+) calM
  • Tailoring (Phosphorylation)
Sequence-based prediction
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  • BAP05586.1
  • calL
15257 - 16411 (+) calL
  • Other enzymatic
Sequence-based prediction
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  • BAP05587.1
  • calK
16776 - 17969 (+) calK
  • Other enzymatic
Sequence-based prediction
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  • BAP05588.1
  • calJ
18377 - 20068 (+) calJ
  • Unknown
Sequence-based prediction
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  • BAP05589.1
  • calA
20404 - 31761 (+) calA
  • Scaffold biosynthesis
Sequence-based prediction
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  • BAP05590.1
  • calB
31828 - 51879 (+) calB
  • Scaffold biosynthesis
Sequence-based prediction
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  • BAP05591.1
  • calC
51876 - 65756 (+) calC
  • Scaffold biosynthesis
Sequence-based prediction
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  • BAP05592.1
  • calD
65768 - 66895 (+) calD
  • Tailoring (Monooxygenation)
Sequence-based prediction
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  • BAP05593.1
  • calE
66955 - 90753 (+) calE
  • Scaffold biosynthesis
Sequence-based prediction
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  • BAP05594.1
  • calF
90764 - 108340 (+) calF
  • Scaffold biosynthesis
Sequence-based prediction
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  • BAP05595.1
  • calG
108337 - 125763 (+) calG
  • Scaffold biosynthesis
Sequence-based prediction
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  • BAP05596.1
  • calH
125766 - 140192 (+) calH
  • Scaffold biosynthesis
Sequence-based prediction
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  • BAP05597.1
  • calI
140205 - 148229 (+) calI
  • Scaffold biosynthesis
Sequence-based prediction
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Polyketide-specific information
Subclass Other
Cyclic? no
Polyketide-synthases
Genes Properties Modules
calY
+
calB
+
calF
+
calI
+
calE
+
calA
+
calC
+
calH
+
calG
Synthase subclass: Trans-AT type I
Trans-acyltansferases: calY
Module 1
Genes: calA
Core domains: Ketosynthase, Thiolation (ACP/PCP)
Scaffold-modifying domains: Methylation
Module 2
Genes: calA
Core domains: Ketosynthase, Thiolation (ACP/PCP)
Scaffold-modifying domains: Methylation
Module 3
Genes: calB
Core domains: Ketosynthase, Thiolation (ACP/PCP)
Module 5
Genes: calB
Core domains: Ketosynthase, Thiolation (ACP/PCP), Dehydratase, Ketoreductase, Enoylreductase
KR-domain stereochemistry: Unknown
Module 6
Genes: calB
Core domains: Ketosynthase, Thiolation (ACP/PCP)
Module 7
Genes: calB
Core domains: Ketosynthase, Thiolation (ACP/PCP)
Module 9
Genes: calC
Core domains: Ketosynthase, Thiolation (ACP/PCP)
Scaffold-modifying domains: Oxidation
Module 10
Genes: calC
Core domains: Ketosynthase, Thiolation (ACP/PCP), Dehydratase, Ketoreductase
Scaffold-modifying domains: Oxidation
KR-domain stereochemistry: Unknown
Module 11
Genes: calC
Core domains: Ketosynthase, Thiolation (ACP/PCP), Ketoreductase
Scaffold-modifying domains: Oxidation
KR-domain stereochemistry: Unknown
Module 12
Genes: calE
Core domains: Ketosynthase, Dehydratase, Thiolation (ACP/PCP)
Module 13
Genes: calE
Core domains: Ketosynthase
Module 14
Genes: calE
Core domains: Ketosynthase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: Unknown
Module 15
Genes: calE
Core domains: Ketosynthase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: Unknown
Module 16
Genes: calE
Core domains: Ketosynthase
Module 17
Genes: calE
Core domains: Ketosynthase, Thiolation (ACP/PCP)
Module 18
Genes: calE
Core domains: Ketosynthase, Ketoreductase
KR-domain stereochemistry: Unknown
Module 19
Genes: calF
Core domains: Ketosynthase, Thiolation (ACP/PCP)
Module 20
Genes: calF
Core domains: Ketosynthase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: Unknown
Module 21
Genes: calF
Core domains: Ketosynthase, Thiolation (ACP/PCP)
Module 22
Genes: calF
Core domains: Ketosynthase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: Unknown
Module 23
Genes: calF, calG
Core domains: Ketosynthase, Ketoreductase, Thiolation (ACP/PCP)
Scaffold-modifying domains: Methylation
KR-domain stereochemistry: Unknown
Module 24
Genes: calG
Core domains: Ketosynthase, Dehydratase, Ketoreductase, Thiolation (ACP/PCP)
Scaffold-modifying domains: Methylation
KR-domain stereochemistry: Unknown
Module 25
Genes: calG
Core domains: Ketosynthase
Scaffold-modifying domains: Methylation
Module 26
Genes: calG
Core domains: Ketosynthase, Dehydratase, Ketoreductase, Thiolation (ACP/PCP)
Scaffold-modifying domains: Methylation
KR-domain stereochemistry: Unknown
Module 27
Genes: calG
Core domains: Ketosynthase
Scaffold-modifying domains: Methylation
Module 28
Genes: calH
Core domains: Ketosynthase
Scaffold-modifying domains: Beta-branching
Module 30
Genes: calH
Core domains: Ketosynthase, Dehydratase, Ketoreductase
Scaffold-modifying domains: Beta-branching
KR-domain stereochemistry: Unknown
Module 31
Genes: calH, calI
Core domains: Ketosynthase, Dehydratase, Thiolation (ACP/PCP)
Scaffold-modifying domains: Beta-branching
Module 32
Genes: calI
Core domains: Ketosynthase
NRP-specific information
Subclass N/A
Cyclic? no
Thioesterase genes
  • calI (Type I)
NRP-synthases
Gene Modules
calA
Module 0
Specificity: serine
Evidence for specificity:
  • Sequence-based prediction
Modification domains: Methylation
calB
Module 4
Specificity: glycine
Evidence for specificity:
  • Sequence-based prediction
Condensation domain type: LCL
Module 8
Condensation domain type: LCL
Modification domains: Unknown
calC
Module 8
Specificity: serine
Evidence for specificity:
  • Sequence-based prediction
calH
Module 29
Specificity: alanine
Evidence for specificity:
  • Sequence-based prediction
Condensation domain type: LCL
calI
Module 33
Specificity: serine
Evidence for specificity:
  • Sequence-based prediction
Condensation domain type: LCL
Annotation changelog
MIBiG version Submitter Notes
1.0
  • Hidden contributor (ID: CUIZDUBUBVAF27VHF4OZXTI3, no GDPR consent given).
  • Submitted
2.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Migrated from v1.4
  • Updated compound(s) information (NPAtlas curation)
3.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Corrected NRP module activity
  • Removed ketoreductase stereochemistry annotation from modules without ketoreductases
  • Merged cross-CDS PKS modules
3.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Changed amino acid substrates to lower case
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