BGC0000969 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000969 (Chondromyces crocatus)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS,NRPS	1	49,145	chondramide A	NRPS:Type I+PKS:Type I	100%

BGC0000969 - Region 1 - NRPS,T1PKS

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Location: 1 - 49,145 nt. (total: 49,145 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: cds(Condensation and (AMP-binding or A-OX))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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TTA codons

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 49,146
Label		BGC0000969

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000969	1.95	NRP, Polyketide	chondramide A	Chondromyces crocatus
BGC0000426	0.76	NRP	sevadicin	Paenibacillus larvae
BGC0001133	0.74	NRP	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0001187	0.73	NRP, Polyketide	xenolozoyenone	Glarea lozoyensis
BGC0000041	0.71	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0002071	0.69	NRP	virginiafactin A, virginiafactin B, virginiafactin C, virginiafactin D	Pseudomonas sp. QS1027
BGC0002608	0.69	NRP, Polyketide	ochratoxin A	Aspergillus steynii
BGC0002341	0.67	Other	3,5-dibromo-p-anisic acid	Planctomycetales bacterium 10988
BGC0000465	0.66	NRP	xenortide A, xenortide B, xenortide C, xenortide D	Xenorhabdus nematophila ATCC 19061
BGC0001023	0.66	NRP, Polyketide	myxochromide S1	Stigmatella aurantiaca

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000969	1.00	NRP, Polyketide	chondramide A	Chondromyces crocatus
BGC0000426	0.70	NRP	sevadicin	Paenibacillus larvae
BGC0001133	0.70	NRP	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0001187	0.69	NRP, Polyketide	xenolozoyenone	Glarea lozoyensis
BGC0000041	0.69	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0002071	0.67	NRP	virginiafactin A, virginiafactin B, virginiafactin C, virginiafactin D	Pseudomonas sp. QS1027
BGC0002608	0.67	NRP, Polyketide	ochratoxin A	Aspergillus steynii
BGC0002341	0.67	Other	3,5-dibromo-p-anisic acid	Planctomycetales bacterium 10988
BGC0000465	0.66	NRP	xenortide A, xenortide B, xenortide C, xenortide D	Xenorhabdus nematophila ATCC 19061
BGC0001023	0.66	NRP, Polyketide	myxochromide S1	Stigmatella aurantiaca

Similar known gene clusters from MIBiG 4.0

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 49145:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-ccmal) + (Me-redmal) + (Ala) + (D-NMe-Trp - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

cmdA

PKS_AT (626..914): pk

ATSignature: Benzoyl-CoA

Top 3 matches:: Benzoyl-CoA: 75.0%; Methylmalonyl-CoA: 58.3%; Ethylmalonyl-CoA: 54.2%

minowa: Benzoyl-CoA

Prediction, score:

Benzoyl-CoA: 147.1
Isobutyryl-CoA: 123.7
Methylmalonyl-CoA: 121.2
Ethylmalonyl-CoA: 109.5
Methoxymalonyl-CoA: 104.4
Malonyl-CoA: 71.7
Propionyl-CoA: 66.6
CHC-CoA: 59.5
trans-1,2-CPDA: 58.1
2-Methylbutyryl-CoA: 58.0
Acetyl-CoA: 57.1
fatty_acid: 33.4
3-Methylbutyryl-CoA: 31.7
inactive: 31.3

PKS_AT (1054..1354): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Malonyl-CoA: 87.5%; Ethylmalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 175.9
Ethylmalonyl-CoA: 150.4
Methoxymalonyl-CoA: 136.0
Benzoyl-CoA: 105.2
Malonyl-CoA: 103.9
Isobutyryl-CoA: 101.3
2-Methylbutyryl-CoA: 96.9
Propionyl-CoA: 82.6
trans-1,2-CPDA: 73.6
Acetyl-CoA: 67.8
CHC-CoA: 55.5
3-Methylbutyryl-CoA: 46.8
inactive: 27.8
fatty_acid: 26.5

PKS_AT (2859..3154): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Ethylmalonyl-CoA: 87.5%; Malonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 173.1
Ethylmalonyl-CoA: 145.0
Methoxymalonyl-CoA: 132.2
Isobutyryl-CoA: 113.5
Malonyl-CoA: 105.2
Benzoyl-CoA: 102.0
2-Methylbutyryl-CoA: 96.8
Propionyl-CoA: 77.8
trans-1,2-CPDA: 76.8
Acetyl-CoA: 61.0
CHC-CoA: 55.2
3-Methylbutyryl-CoA: 53.8
inactive: 41.2
fatty_acid: 25.7

cmdB

PKS_AT (566..853): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Ethylmalonyl-CoA: 87.5%; Malonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 170.3
Ethylmalonyl-CoA: 146.5
Methoxymalonyl-CoA: 127.8
Benzoyl-CoA: 109.6
Isobutyryl-CoA: 105.5
Malonyl-CoA: 83.7
2-Methylbutyryl-CoA: 80.2
trans-1,2-CPDA: 76.9
Propionyl-CoA: 66.0
Acetyl-CoA: 61.3
CHC-CoA: 60.8
3-Methylbutyryl-CoA: 44.2
inactive: 29.3
fatty_acid: 25.5

cmdC

Search NORINE for peptide: strict or relaxed

AMP-binding (530..920): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: ala (Ala)

Stachelhaus prediction details:

Stachelhaus sequence:: DLFNNALTYK
Nearest Stachelhaus code(s):: Ala DLFNNALTYK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

cmdD

Search NORINE for peptide: strict or relaxed

AMP-binding (485..887): Trp

nrpys: Trp

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DGVQMAGVGK
Nearest Stachelhaus code(s):: Trp DGVQMAGVGK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2480..2885): X

nrpys: bTyr, Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DGSTITAVAK
Nearest Stachelhaus code(s):: bTyr or Tyr DGSTITAVAK (100% 8Å match)
Stachelhaus code match:: 100% (strong)