BGC0000972: N-myristoyl-D-asparagine biosynthetic gene cluster from Escherichia coli
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Location: 1 - 55,140 nt. (total: 55,140 nt).
This entry is originally from NCBI GenBank AM229678.1.

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
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Gene details
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General
Compounds
Genes
Polyketide
NRP
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0000972
Short description N-myristoyl-D-asparagine biosynthetic gene cluster from Escherichia coli
Status Minimal annotation: no
A minimal annotation only contains information on the BGC loci and one or more linked chemical product(s)

Completeness: complete
Whether the loci encodes everything needed for the pathway producing the compound(s)
Biosynthetic class(es)
  • NRP
  • Polyketide (Other)
Loci NCBI GenBank: AM229678.1
Compounds
  • N-myristoyl-D-asparagine
  • cis-7-tetradecenoyl-D-asparagine
  • (R)-N1-((S)-5-oxohexan-2-yl)-2-tetradecanamidosuccinamide
Species Escherichia coli [taxonomy]
References
Chemical products information
N-myristoyl-D-asparagine
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C18H34N2O4
cis-7-tetradecenoyl-D-asparagine
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C18H32N2O4
(R)-N1-((S)-5-oxohexan-2-yl)-2-tetradecanamidosuccinamide
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C24H45N3O4
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • CAJ76281.1
  • intP4
 469 - 1740 (+) bacteriophage integrase
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  • CAJ76282.1
 2020 - 2532 (-) hypothetical protein
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  • CAJ76283.1
  • clbQ
 2567 - 3289 (-) putative thioesterase
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  • CAJ76284.1
  • clbP
 3282 - 4796 (-) putative penicillin binding protein
  • Tailoring (Hydrolysis)
Activity assay
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  • CAJ76285.1
  • clbO
 4809 - 7268 (-) putative polyketide synthase
  • Scaffold biosynthesis
Sequence-based prediction
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  • CAJ76286.1
  • clbN
 7299 - 11666 (-) putative non-ribosomal peptide synthetase
  • Scaffold biosynthesis
Activity assay
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  • CAJ76287.1
  • clbM
 11663 - 13102 (-) putative drug/sodium antiporter
  • Transport
Sequence-based prediction
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  • CAJ76288.1
  • clbL
 13164 - 14627 (-) putative amidase
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  • CAJ76289.1
  • clbK
 14620 - 21084 (-) putative hybrid non-ribosomal peptide-polyketide synthetase
  • Scaffold biosynthesis
Sequence-based prediction
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  • CAJ76290.1
  • clbJ
 21095 - 27595 (-) putative non-ribosomal peptide synthase
  • Scaffold biosynthesis
Sequence-based prediction
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  • CAJ76291.1
  • clbI
 27639 - 30671 (-) putative polyketide synthase
  • Scaffold biosynthesis
Sequence-based prediction
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  • CAJ76292.1
  • clbH
 30721 - 35517 (-) putative non-ribosomal peptide synthase
  • Scaffold biosynthesis
Sequence-based prediction
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  • CAJ76293.1
  • clbG
 35565 - 36833 (-) putative malonyl-CoA transacylase
  • Activation / processing
Sequence-based prediction
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  • CAJ76294.1
  • clbF
 36830 - 37960 (-) putative acyl-CoA dehydrogenase
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  • CAJ76295.1
  • clbE
 37964 - 38212 (-) putative D-alanyl carrier protein
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  • CAJ76296.1
  • clbD
 38242 - 39111 (-) putative 3-hydroxyacyl-CoA dehydrogenase
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  • CAJ76297.1
  • clbC
 39121 - 41721 (-) putative polyketide synthase
  • Scaffold biosynthesis
Sequence-based prediction
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  • CAJ76298.1
  • clbB
 41762 - 51382 (-) putative hybrid polyketide-non-ribosomal peptide synthetase
  • Scaffold biosynthesis
Activity assay
Sequence-based prediction
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  • CAJ76299.1
  • clbR
 51840 - 52052 (+) putative regulatory protein
  • Regulation
Sequence-based prediction
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  • CAJ76300.1
  • clbA
 52053 - 52787 (+) putative 4'-phosphopantetheinyl transferase
  • Activation / processing
Sequence-based prediction
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  • CAJ76301.1
 52933 - 53199 (+) IS1400 transposase A
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  • CAJ76302.1
 53304 - 53783 (+) IS1400 transposase B
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  • CAJ76303.1
 53774 - 54001 (+) putative transposase
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Polyketide-specific information
Subclass Other
Cyclic? no
Polyketide-synthases
Genes Properties Modules
clbB
+
clbO
+
clbI
+
clbC
+
clbK 		Synthase subclass: Modular type I/Trans-AT type I
Module ?
Genes: clbC
Core domains: Ketosynthase, Thiolation (ACP/PCP)
Module ?
Specificity: Malonyl-CoA
Evidence for specificity: Sequence-based prediction
Genes: clbI
Core domains: Ketosynthase, Acyltransferase, Thiolation (ACP/PCP)
Module ?
Genes: clbK
Core domains: Ketosynthase, Thiolation (ACP/PCP)
Module ?
Genes: clbO
Core domains: Ketosynthase
Module 3
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: clbB
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Dehydratase, Enoylreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: Unknown
NRP-specific information
Subclass N/A
Cyclic? no
Thioesterase genes
  • clbQ (Unknown)
NRP-synthases
Gene Modules
clbB
Module 2
Specificity: alanine / valine
Evidence for specificity:
  • ATP-PPi exchange assay
Condensation domain type: DCL
clbH
Module ?
Specificity: serine
Evidence for specificity:
  • ATP-PPi exchange assay
Module ?
Specificity: S-adenosylmethionine
Evidence for specificity:
  • ATP-PPi exchange assay
Condensation domain type: LCL
clbJ
Module ?
Specificity: glycine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: LCL
Module ?
Specificity: cysteine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: Heterocyclization
clbK
Module ?
Specificity: cysteine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: Heterocyclization
clbN
Module 1
Specificity: asparagine
Evidence for specificity:
  • ATP-PPi exchange assay
Condensation domain type: Starter
Annotation changelog
MIBiG version Submitter Notes
1.0
  • Hidden contributor (ID: AK6U5FV25F7Z3GQ43DVTSAVK, no GDPR consent given).
  • Submitted
2.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Migrated from v1.4
3.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Corrected NRP module activity
  • Removed duplicate gene name
  • Removed ketoreductase stereochemistry annotation from modules without ketoreductases
  • Sorted modules by module number
  • Corrected gene identifiers
  • Added NRP module specificity
  • Updated NRP substrate specificities
3.1
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Update chemical activity to schema version 2.11
Detailed domain annotation
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