BGC0000972: N-myristoyl-D-asparagine biosynthetic gene cluster from Escherichia coli
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Location: 1 - 55,140 nt. (total: 55,140 nt).
This entry is originally from NCBI GenBank AM229678.1.

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
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Gene details
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General
Compounds
Genes
Biosynthesis
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0000972
Short description N-myristoyl-D-asparagine biosynthetic gene cluster from Escherichia coli
Status Quality: questionable
The quality level of this entry.

Status: active
The status of this entry.

Completeness: complete
Whether the entry covers everything needed for the pathway producing the compound(s)
Biosynthetic class(es)
  • NRPS (Type I)
  • PKS (Type I)
Loci
AM229678.1
via Knock-out studies, Heterologous expression
Compounds
  • N-myristoyl-D-asparagine
  • cis-7-tetradecenoyl-D-asparagine
  • (R)-N1-((S)-5-oxohexan-2-yl)-2-tetradecanamidosuccinamide
  • colibactin
  • precolibactin 1489
Species Escherichia coli [taxonomy]
References
Chemical products information
N-myristoyl-D-asparagine Evidence:
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C18H34N2O4
Chemical database entries
PubCHEM
cis-7-tetradecenoyl-D-asparagine Evidence:
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C18H32N2O4
Chemical database entries
PubCHEM
(R)-N1-((S)-5-oxohexan-2-yl)-2-tetradecanamidosuccinamide Evidence:
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C24H45N3O4
Chemical database entries
PubCHEM
colibactin Evidence:
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Chemical database entries
PubCHEM
ChEBI
precolibactin 1489 Evidence:
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List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • CAJ76281.1
  • intP4
 469 - 1740 (+) bacteriophage integrase
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    • CAJ76282.1
    		 2020 - 2532 (-) hypothetical protein
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      • CAJ76283.1
      • clbQ
      		 2567 - 3289 (-) putative thioesterase
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        • CAJ76284.1
        • clbP
        		 3282 - 4796 (-) putative penicillin binding protein
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          • CAJ76285.1
          • clbO
          		 4809 - 7268 (-) putative polyketide synthase
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            • CAJ76286.1
            • clbN
            		 7299 - 11666 (-) putative non-ribosomal peptide synthetase
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              • CAJ76287.1
              • clbM
              		 11663 - 13102 (-) putative drug/sodium antiporter
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                • CAJ76288.1
                • clbL
                		 13164 - 14627 (-) putative amidase
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                  • CAJ76289.1
                  • clbK
                  		 14620 - 21084 (-) putative hybrid non-ribosomal peptide-polyketide synthetase
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                    • CAJ76290.1
                    • clbJ
                    		 21095 - 27595 (-) putative non-ribosomal peptide synthase
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                      • CAJ76291.1
                      • clbI
                      		 27639 - 30671 (-) putative polyketide synthase
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                        • CAJ76292.1
                        • clbH
                        		 30721 - 35517 (-) putative non-ribosomal peptide synthase
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                          • CAJ76293.1
                          • clbG
                          		 35565 - 36833 (-) putative malonyl-CoA transacylase
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                            • CAJ76294.1
                            • clbF
                            		 36830 - 37960 (-) putative acyl-CoA dehydrogenase
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                              • CAJ76295.1
                              • clbE
                              		 37964 - 38212 (-) putative D-alanyl carrier protein
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                                • CAJ76296.1
                                • clbD
                                		 38242 - 39111 (-) putative 3-hydroxyacyl-CoA dehydrogenase
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                                  • CAJ76297.1
                                  • clbC
                                  		 39121 - 41721 (-) putative polyketide synthase
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                                    • CAJ76298.1
                                    • clbB
                                    		 41762 - 51382 (-) putative hybrid polyketide-non-ribosomal peptide synthetase
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                                      • CAJ76299.1
                                      • clbR
                                      		 51840 - 52052 (+) putative regulatory protein
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                                        • CAJ76300.1
                                        • clbA
                                        		 52053 - 52787 (+) putative 4'-phosphopantetheinyl transferase
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                                          • CAJ76301.1
                                          		 52933 - 53199 (+) IS1400 transposase A
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                                            • CAJ76302.1
                                            		 53304 - 53783 (+) IS1400 transposase B
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                                              • CAJ76303.1
                                              		 53774 - 54001 (+) putative transposase
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                                                Biosynthesis information

                                                Biosynthetic modules

                                                Name
                                                2
                                                Type
                                                nrps-type1
                                                Genes
                                                clbB
                                                Substrates
                                                alanine, valine (evidence: ATP-PPi exchange assay [1])
                                                Integrated Monomers
                                                Domains
                                                condensation (DCL), adenylation
                                                Name
                                                Unk01
                                                Type
                                                nrps-type1
                                                Genes
                                                clbH
                                                Substrates
                                                serine (evidence: ATP-PPi exchange assay [2])
                                                Integrated Monomers
                                                Domains
                                                adenylation
                                                Name
                                                Unk02
                                                Type
                                                nrps-type1
                                                Genes
                                                clbH
                                                Substrates
                                                S-adenosylmethionine (evidence: ATP-PPi exchange assay [2])
                                                Integrated Monomers
                                                Domains
                                                condensation (LCL), adenylation
                                                Name
                                                Unk03
                                                Type
                                                nrps-type1
                                                Genes
                                                clbJ
                                                Substrates
                                                glycine (evidence: Structure-based inference [3])
                                                Integrated Monomers
                                                Domains
                                                condensation (LCL), adenylation
                                                Name
                                                Unk04
                                                Type
                                                nrps-type1
                                                Genes
                                                clbJ
                                                Substrates
                                                cysteine (evidence: Structure-based inference [3])
                                                Integrated Monomers
                                                Domains
                                                condensation (Heterocyclization), adenylation
                                                Name
                                                Unk05
                                                Type
                                                nrps-type1
                                                Genes
                                                clbK
                                                Substrates
                                                cysteine (evidence: Structure-based inference [3])
                                                Integrated Monomers
                                                Domains
                                                condensation (Heterocyclization), adenylation
                                                Name
                                                1
                                                Type
                                                nrps-type1
                                                Genes
                                                clbN
                                                Substrates
                                                asparagine (evidence: ATP-PPi exchange assay [1])
                                                Integrated Monomers
                                                Domains
                                                condensation (Starter), adenylation, epimerase
                                                Name
                                                None
                                                Type
                                                pks-trans-at
                                                Genes
                                                clbC
                                                Substrates
                                                Integrated Monomers
                                                Domains
                                                ketosynthase, carrier (ACP)
                                                Name
                                                None
                                                Type
                                                pks-modular
                                                Genes
                                                clbI
                                                Substrates
                                                Integrated Monomers
                                                Domains
                                                acyltransferase, ketosynthase, acyltransferase, carrier (ACP)
                                                Name
                                                None
                                                Type
                                                pks-trans-at
                                                Genes
                                                clbK
                                                Substrates
                                                Integrated Monomers
                                                Domains
                                                ketosynthase, carrier (ACP)
                                                Name
                                                None
                                                Type
                                                pks-trans-at
                                                Genes
                                                clbO
                                                Substrates
                                                Integrated Monomers
                                                Domains
                                                ketosynthase
                                                Name
                                                3
                                                Type
                                                pks-modular
                                                Genes
                                                clbB
                                                Substrates
                                                Integrated Monomers
                                                Domains
                                                acyltransferase, ketosynthase, acyltransferase, ketoreductase, dehydratase, enoylreductase, carrier (ACP)
                                                Annotation changelog

                                                Entry version: next

                                                Date
                                                Changes
                                                Submitters
                                                Reviewers
                                                2024-03-19
                                                MIBiG v4 annotathon
                                                • (ID: SJG3CBEHSM6A3I74YXLH6GLA)
                                                • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                                                Entry version: 4

                                                Date
                                                Changes
                                                Submitters
                                                Reviewers
                                                2022-10-07
                                                Update chemical activity to schema version 2.11
                                                • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                                                Entry version: 3

                                                Date
                                                Changes
                                                Submitters
                                                Reviewers
                                                2022-09-15
                                                Corrected NRP module activity
                                                • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                2022-09-15
                                                Removed duplicate gene name
                                                • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                2022-09-15
                                                Removed ketoreductase stereochemistry annotation from modules without ketoreductases
                                                • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                2022-09-15
                                                Sorted modules by module number
                                                • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                2022-09-15
                                                Corrected gene identifiers
                                                • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                2022-09-15
                                                Added NRP module specificity
                                                • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                2022-09-15
                                                Updated NRP substrate specificities
                                                • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                                                Entry version: 2

                                                Date
                                                Changes
                                                Submitters
                                                Reviewers
                                                2019-10-16
                                                Migrated from v1.4
                                                • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                                                Entry version: 1

                                                Date
                                                Changes
                                                Submitters
                                                Reviewers
                                                2015-06-12
                                                Submitted
                                                • (ID: AK6U5FV25F7Z3GQ43DVTSAVK)
                                                • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                Detailed domain annotation
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