BGC0000978 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000978 (Cylindrospermopsis raciborskii AWT205)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	NRPS-like,T1PKS	1	42,156	cylindrospermopsin	NRPS:Type I+PKS:Type I+other:other	100%

BGC0000978 - Region 1 - NRPS-like,T1PKS

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Location: 1 - 42,156 nt. (total: 42,156 nt) Show pHMM detection rules used

Region on contig edge.

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 42,157
Label		BGC0000978

Detailed domain annotation

Selected features only

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Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000978	2.00	NRP, Polyketide, Alkaloid	cylindrospermopsin	Cylindrospermopsis raciborskii AWT205
BGC0000979	1.03	NRP, Polyketide	cylindrospermopsin	Aphanizomenon sp. 10E9
BGC0000980	1.03	NRP, Polyketide	cylindrospermopsin	Aphanizomenon sp. 22D11
BGC0000981	0.75	NRP, Polyketide	cylindrospermopsin	Aphanizomenon sp. 10E6
BGC0001164	0.73	Polyketide	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001163	0.67	Polyketide	1-heptadecene	Moorea producens 3L
BGC0001161	0.54	Polyketide	1-heptadecene	Moorea bouillonii PNG
BGC0001162	0.53	Polyketide	1-heptadecene	Moorea producens JHB
BGC0002171	0.51	NRP, Polyketide	pyrophen, campyrone B	Aspergillus niger ATCC 1015
BGC0002275	0.51	NRP, Polyketide	pyrophen, campyrone B	Aspergillus niger CBS 101883

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000978	1.00	NRP, Polyketide, Alkaloid	cylindrospermopsin	Cylindrospermopsis raciborskii AWT205
BGC0000979	0.80	NRP, Polyketide	cylindrospermopsin	Aphanizomenon sp. 10E9
BGC0000980	0.80	NRP, Polyketide	cylindrospermopsin	Aphanizomenon sp. 22D11
BGC0000981	0.70	NRP, Polyketide	cylindrospermopsin	Aphanizomenon sp. 10E6
BGC0001164	0.69	Polyketide	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001163	0.67	Polyketide	1-heptadecene	Moorea producens 3L
BGC0001161	0.61	Polyketide	1-heptadecene	Moorea bouillonii PNG
BGC0001162	0.60	Polyketide	1-heptadecene	Moorea producens JHB
BGC0002171	0.59	NRP, Polyketide	pyrophen, campyrone B	Aspergillus niger ATCC 1015
BGC0002275	0.59	NRP, Polyketide	pyrophen, campyrone B	Aspergillus niger CBS 101883

Similar known gene clusters from MIBiG 4.0

Module view

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 42156:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (ccmal) + (mal) + (X - Me-ccmal) + (ccmal) + (ohmal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

cyrD

PKS_AT (590..885): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 95.8%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 190.8
inactive: 182.5
Methoxymalonyl-CoA: 48.6
Methylmalonyl-CoA: 45.0
Propionyl-CoA: 37.9
Isobutyryl-CoA: 18.1
2-Methylbutyryl-CoA: 14.3
Benzoyl-CoA: 13.4
Acetyl-CoA: 9.2
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

cyrF

PKS_AT (553..855): mal

ATSignature: Malonyl-CoA, inactive

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 100.0%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 191.7
inactive: 176.4
Methoxymalonyl-CoA: 55.1
Methylmalonyl-CoA: 44.8
Propionyl-CoA: 34.8
Isobutyryl-CoA: 27.9
Acetyl-CoA: 19.1
Benzoyl-CoA: 18.1
trans-1,2-CPDA: 18.0
Ethylmalonyl-CoA: 12.3
3-Methylbutyryl-CoA: 10.9
CHC-CoA: 9.9
2-Methylbutyryl-CoA: 6.2
fatty_acid: 0.0

cyrB

AMP-binding (28..469): X

nrpys: Gua

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DFHFITHDAK
Nearest Stachelhaus code(s):: Gua DFHFITHDAK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

PKS_AT (1210..1512): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 95.8%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 216.5
inactive: 184.5
Methoxymalonyl-CoA: 74.8
Methylmalonyl-CoA: 62.4
Propionyl-CoA: 40.9
Isobutyryl-CoA: 28.9
Benzoyl-CoA: 26.2
Ethylmalonyl-CoA: 25.8
Acetyl-CoA: 19.4
2-Methylbutyryl-CoA: 18.8
CHC-CoA: 16.3
trans-1,2-CPDA: 13.8
fatty_acid: 11.3
3-Methylbutyryl-CoA: 8.3

cyrE

PKS_AT (548..850): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 95.8%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 214.4
inactive: 185.8
Methoxymalonyl-CoA: 70.4
Methylmalonyl-CoA: 60.1
Propionyl-CoA: 52.2
Acetyl-CoA: 38.6
Isobutyryl-CoA: 35.1
2-Methylbutyryl-CoA: 29.2
Benzoyl-CoA: 22.5
trans-1,2-CPDA: 22.4
Ethylmalonyl-CoA: 20.4
fatty_acid: 13.0
CHC-CoA: 11.2
3-Methylbutyryl-CoA: 10.6

cyrC

PKS_AT (568..879): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 95.8%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 205.3
inactive: 169.4
Methoxymalonyl-CoA: 67.6
Methylmalonyl-CoA: 67.5
Propionyl-CoA: 42.8
Isobutyryl-CoA: 41.2
Benzoyl-CoA: 31.4
trans-1,2-CPDA: 24.4
Acetyl-CoA: 22.7
2-Methylbutyryl-CoA: 21.2
Ethylmalonyl-CoA: 21.1
CHC-CoA: 19.5
fatty_acid: 10.0
3-Methylbutyryl-CoA: 8.7