BGC0000982 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000982 (Cystobacter fuscus)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS,NRPS	1	63,876	cystothiazole A	NRPS:Type I+PKS	100%

BGC0000982 - Region 1 - NRPS,T1PKS

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Location: 1 - 63,876 nt. (total: 63,876 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: cds(Condensation and (AMP-binding or A-OX))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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resistance

TTA codons

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 63,877
Label		BGC0000982

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000982	2.00	NRP, Polyketide	cystothiazole A	Cystobacter fuscus
BGC0001024	0.94	NRP, Polyketide	myxothiazol	Stigmatella aurantiaca DW4/3-1
BGC0001010	0.77	NRP, Polyketide	melithiazol A	Melittangium lichenicola
BGC0001704	0.64	Other	nocuolin A	Nodularia sp. HBU26
BGC0002597	0.64	NRP, Polyketide	nocuolactylates	Nodularia sp. LEGE 06071
BGC0000041	0.59	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0000373	0.59	NRP	holomycin	Streptomyces clavuligerus ATCC 27064
BGC0000895	0.58	Other	citrulline	Lactobacillus plantarum subsp. plantarum
BGC0001023	0.58	NRP, Polyketide	myxochromide S1	Stigmatella aurantiaca
BGC0001941	0.56	NRP, Polyketide	fulvuthiacene A, fulvuthiacene B	Myxococcus fulvus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000982	1.00	NRP, Polyketide	cystothiazole A	Cystobacter fuscus
BGC0001024	0.77	NRP, Polyketide	myxothiazol	Stigmatella aurantiaca DW4/3-1
BGC0001010	0.71	NRP, Polyketide	melithiazol A	Melittangium lichenicola
BGC0001704	0.65	Other	nocuolin A	Nodularia sp. HBU26
BGC0002597	0.65	NRP, Polyketide	nocuolactylates	Nodularia sp. LEGE 06071
BGC0000041	0.63	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0000373	0.63	NRP	holomycin	Streptomyces clavuligerus ATCC 27064
BGC0000895	0.63	Other	citrulline	Lactobacillus plantarum subsp. plantarum
BGC0001023	0.63	NRP, Polyketide	myxochromide S1	Stigmatella aurantiaca
BGC0001941	0.62	NRP, Polyketide	fulvuthiacene A, fulvuthiacene B	Myxococcus fulvus

Similar known gene clusters from MIBiG 4.0

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 63876:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk) + (Cys) + (Cys - ccmal) + (OMe-Me-ohmal) + (OMe-mal) + (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctaB

PKS_AT (554..848): pk

ATSignature: Isobutyryl-CoA

Top 3 matches:: Isobutyryl-CoA: 100.0%; Methylmalonyl-CoA: 79.2%; Malonyl-CoA: 58.3%

minowa: Isobutyryl-CoA

Prediction, score:

Isobutyryl-CoA: 233.0
Methylmalonyl-CoA: 94.0
Methoxymalonyl-CoA: 83.9
Malonyl-CoA: 83.7
Benzoyl-CoA: 40.4
Propionyl-CoA: 37.5
Ethylmalonyl-CoA: 37.4
3-Methylbutyryl-CoA: 31.7
fatty_acid: 30.6
inactive: 30.2
Acetyl-CoA: 24.6
CHC-CoA: 22.1
2-Methylbutyryl-CoA: 18.7
trans-1,2-CPDA: 7.0

ctaC

Search NORINE for peptide: strict or relaxed

AMP-binding (516..913): Cys

nrpys: Cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: DLYNMSLVWK
Nearest Stachelhaus code(s):: Cys DLYNMSLVWK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

ctaD

Search NORINE for peptide: strict or relaxed

AMP-binding (544..943): Cys

nrpys: Cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: DLYNLSLIWK
Nearest Stachelhaus code(s):: Cys DLYNLSLIWK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

PKS_AT (1983..2275): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 62.5%; Isobutyryl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 161.8
inactive: 110.6
Methoxymalonyl-CoA: 82.6
Methylmalonyl-CoA: 66.6
Propionyl-CoA: 35.5
Isobutyryl-CoA: 29.4
2-Methylbutyryl-CoA: 24.0
Ethylmalonyl-CoA: 23.2
trans-1,2-CPDA: 20.3
CHC-CoA: 18.3
Benzoyl-CoA: 15.5
Acetyl-CoA: 9.6
fatty_acid: 9.4
3-Methylbutyryl-CoA: 7.1

ctaE

PKS_AT (571..867): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Malonyl-CoA: 83.3%; Ethylmalonyl-CoA: 79.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 176.0
Ethylmalonyl-CoA: 145.0
Methoxymalonyl-CoA: 137.7
Isobutyryl-CoA: 133.4
Benzoyl-CoA: 122.2
Malonyl-CoA: 93.8
Acetyl-CoA: 89.1
2-Methylbutyryl-CoA: 88.4
Propionyl-CoA: 75.6
trans-1,2-CPDA: 74.5
CHC-CoA: 66.0
3-Methylbutyryl-CoA: 46.0
fatty_acid: 42.7
inactive: 32.2

ctaF

PKS_AT (568..860): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 62.5%; Isobutyryl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 159.6
inactive: 109.5
Methoxymalonyl-CoA: 82.5
Methylmalonyl-CoA: 66.3
Propionyl-CoA: 35.3
Isobutyryl-CoA: 28.9
2-Methylbutyryl-CoA: 24.0
Ethylmalonyl-CoA: 23.1
trans-1,2-CPDA: 19.7
CHC-CoA: 18.3
Benzoyl-CoA: 15.6
Acetyl-CoA: 11.8
fatty_acid: 9.2
3-Methylbutyryl-CoA: 7.1

ctaG

A-OX (554..1315): X