Genomic loci for this biosynthetic pathway

Cluster Type From To
The following clusters are from record BGC0000983.1:
Cluster 1NRP / Polyketide145018

BGC0000983, cytochalasin biosynthetic gene cluster from Aspergillus clavatus. Locus 1. Full MIBiG entry.

Chemical compounds

Compound: cytochalasin E
PubChem ID: 5458385
SMILES string: Copy to clipboard
Molecular formula: C28H33NO7
Average molecular mass: 495.572 Da
Molecular activity: Inhibitor
Molecular target: angiogenesis

Class-specific details

Biosynthetic class(es):
NRP / Polyketide
Other (cyclic)
Thioesterase type:
None
Release / cyclization type:
Reductive release

Nonribosomal peptide synthetases:
ccsA (EAW09117.1)
Module 2
A specificity: Phenylalanine
Evidence for specificity: Structure-based inference
C domain subtype: LCL
Polyketide subclass:
Other (cyclic)
Polyketide synthase subclass:
Iterative type I
Starter unit:
Acetyl-CoA
Polyketide synthase / ketosynthase-encoding genes:
EAW09117
Iterative PKS subtype:
Highly reducing
Number of iterations:
8
Other
Thioesterase type:
None
Release / cyclization type:
Reductive release

Modular polyketide synthases:
ccsA (EAW09117.1)
Module 1
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: Unknown
Scaffold-modifying domain: Methylation
Module is iterated
Evidence for iteration: Unknown

Gene cluster description

cytochalasin (BGC0000983). Gene Cluster 1. Biosynthetic class = NRP/Polyketide. GenBank DS027057, positions 334000-378500. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Domain annotation

Homologous known gene clusters

General MIBiG information on this cluster

Complete gene cluster sequence?complete
Evidence for cluster-compound connection:Knock-out studies
MIxS-compliance:Unknown
Contact for this cluster:Yit-Heng Chooi (The Australian National University)

Literature references

1. Qiao K et al. (2011) Identification and engineering of the cytochalasin gene cluster from Aspergillus clavatus NRRL 1. Metab Eng 13(6):723-32. doi: 10.1016/j.ymben.2011.09.008. Epub 2011 Oct
2. Udagawa T et al. (2000) Cytochalasin E, an epoxide containing Aspergillus-derived fungal metabolite, inhibits angiogenesis and tumor growth. J Pharmacol Exp Ther 294(2):421-7.