BGC0000985: didemnin B biosynthetic gene cluster from Tistrella mobilis KA081020-065
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Location: 674,941 - 729,697 nt. (total: 54,757 nt).
This entry is originally from NCBI GenBank CP003239.1.

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
TTA codons
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Gene details
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General
Compounds
Genes
Polyketide
NRP
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0000985
Short description didemnin B biosynthetic gene cluster from Tistrella mobilis KA081020-065
Status Minimal annotation: no
A minimal annotation only contains information on the BGC loci and one or more linked chemical product(s)

Completeness: complete
Whether the loci encodes everything needed for the pathway producing the compound(s)
Remarks "Analysis of genes flanking didA-J, and within the Tistrella mobilis genome, did not reveal a discrete AT suggesting the Didemnin polyketide synthase may use the fatty acid synthase FabD"
Biosynthetic class(es)
  • NRP (Cyclic depsipeptide)
  • Polyketide (Other)
Loci NCBI GenBank: CP003239.1
Compounds
  • didemnin B
  • nordidemnin B
  • dehydrodidemnin B (aplidine)
  • didemnin X
  • didemnin Y
Species Tistrella mobilis KA081020-065 [taxonomy]
References
Chemical products information
didemnin B
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C57H89N7O15
Chemical database entries
PubCHEM
nordidemnin B [synonyms: Nordidemnin]
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C56H87N7O15
Chemical database entries
PubCHEM
PubCHEM
dehydrodidemnin B (aplidine)
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C56H87N7O15
didemnin X
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C82H130N12O24
Chemical database entries
PubCHEM
didemnin Y
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C87H138N14O26
Chemical database entries
PubCHEM
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • TMO_c0602
  • AFK57212.1
  • didA
 674941 - 681312 (+) DidA
  • Scaffold biosynthesis
Sequence-based prediction
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  • TMO_c0603
  • AFK57213.1
  • didB
 681309 - 686699 (+) DidB
  • Scaffold biosynthesis
Sequence-based prediction
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  • TMO_c0604
  • AFK57214.1
  • didC
 686696 - 690688 (+) DidC
  • Scaffold biosynthesis
Sequence-based prediction
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  • TMO_c0605
  • AFK57215.1
  • didD
 690664 - 702225 (+) DidD
  • Scaffold biosynthesis
Sequence-based prediction
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  • TMO_c0606
  • AFK57216.1
  • didE
 702250 - 707367 (+) DidE
  • Scaffold biosynthesis
Sequence-based prediction
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  • TMO_c0607
  • AFK57217.1
  • didF
 707383 - 712224 (+) DidF
  • Scaffold biosynthesis
Sequence-based prediction
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  • TMO_c0608
  • AFK57218.1
  • didG
 712221 - 716462 (+) DidG
  • Scaffold biosynthesis
Sequence-based prediction
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  • TMO_c0609
  • AFK57219.1
  • didH
 716459 - 720319 (+) DidH
  • Scaffold biosynthesis
Sequence-based prediction
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  • TMO_c0610
  • AFK57220.1
  • didI
 720291 - 722912 (+) DidI
  • Scaffold biosynthesis
Sequence-based prediction
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  • TMO_c0611
  • AFK57221.1
  • didJ
 722909 - 729400 (+) DidJ
  • Scaffold biosynthesis
Sequence-based prediction
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  • TMO_c0612
  • AFK57222.1
  • mbtH
 729464 - 729697 (+) MbtH domain-containing protein
  • Activation / processing
Sequence-based prediction
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Polyketide-specific information
Subclass Other
Cyclic? yes
NRP-specific information
Subclass Cyclic depsipeptide
Cyclic? yes
Release type
  • Macrolactamization
Thioesterase genes
  • didJ (Type I)
NRP-synthases
Gene Modules
didA
Module ?
Specificity: glutamine
Evidence for specificity:
  • Structure-based inference
  • Sequence-based prediction
Module ?
Specificity: glutamine
Evidence for specificity:
  • Structure-based inference
  • Sequence-based prediction
didC
Module ?
Specificity: proline
Evidence for specificity:
  • Structure-based inference
  • Sequence-based prediction
didD
Module ?
Specificity: leucine
Evidence for specificity:
  • Structure-based inference
  • Sequence-based prediction
Module ?
Specificity: threonine
Evidence for specificity:
  • Structure-based inference
  • Sequence-based prediction
Module ?
Specificity: isoleucine
Evidence for specificity:
  • Structure-based inference
  • Sequence-based prediction
didH
Module ?
Specificity: leucine
Evidence for specificity:
  • Structure-based inference
  • Sequence-based prediction
didI
Module ?
Specificity: proline
Evidence for specificity:
  • Structure-based inference
  • Sequence-based prediction
didJ
Module ?
Specificity: tyrosine
Evidence for specificity:
  • Structure-based inference
  • Sequence-based prediction
Annotation changelog
MIBiG version Submitter Notes
1.0
  • Hidden contributor (ID: FC27FLXW4TUBR6P2D2MHHJOO, no GDPR consent given).
  • Submitted
2.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Migrated from v1.4
  • Updated compound(s) information (MIBiG Annotathon)
  • Updated compound(s) information (NPAtlas curation)
3.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Hidden contributor (ID: 5UL74VURKJ25VSPPPO3H2NYB, no GDPR consent given).
  • Cleaned up synonym list
  • Update compound structure (SMILES) for nordidemnin B
  • Linked new PubChem id for nordidemnin B (pubchem:164049)
  • Add new compound: dehydrodidemnin B (aplidine)
  • Updated bioactivity data
  • Added NRP substrate specificities
3.1
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Update chemical activity to schema version 2.11
Detailed domain annotation
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