BGC0000985 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000985 (Tistrella mobilis KA081020-065)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	NRPS	1	94,460	High	didemnin B/nordidemnin B/dehydrodidemnin B (aplidine)/didemnin X/didemnin Y	NRPS:Type I+PKS

BGC0000985 - Region 1 - NRPS

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Location: 1 - 94,460 nt. (total: 94,460 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 94,461
Label		BGC0000985

Tailoring functions

Transferases: acyltransferase

TMO_c0609: polyketide synthase associated protein papA3

smcogs: SMCOG1209: polyketide synthase associated protein papA3

TMO_c0611: polyketide synthase associated protein papA3

smcogs: SMCOG1209: polyketide synthase associated protein papA3

Ligases: ligase synthase

TMO_c0602: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

TMO_c0603: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

TMO_c0604: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

TMO_c0605: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

TMO_c0606: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

TMO_c0608: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

TMO_c0610: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000985.5	0.97	NRPS, PKS	didemnin B, nordidemnin B, dehydrodidemnin B (aplidine), didemnin X, didemnin Y	Tistrella mobilis KA081020-065
BGC0001135.5	0.75	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001758.4	0.74	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0002135.2	0.74	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001128.4	0.74	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001249.5	0.73	NRPS	dimethylcoprogen	Alternaria alternata
BGC0001132.4	0.73	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001833.4	0.73	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001050.5	0.73	NRPS, PKS	thalassospiramide A	Tistrella bauzanensis
BGC0002286.3	0.73	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000985.5	0.97	NRPS, PKS	didemnin B, nordidemnin B, dehydrodidemnin B (aplidine), didemnin X, didemnin Y	Tistrella mobilis KA081020-065
BGC0001135.5	0.75	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001758.4	0.74	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0002135.2	0.74	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001128.4	0.74	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001249.5	0.73	NRPS	dimethylcoprogen	Alternaria alternata
BGC0001132.4	0.73	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001833.4	0.73	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001050.5	0.73	NRPS, PKS	thalassospiramide A	Tistrella bauzanensis
BGC0002286.3	0.73	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 94460:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Gln - Gln) + (AHBA) + (Pro - D-NMe-Leu) + (Thr - Ile) + (shikimic_acid) + (Leu) + (Pro) + (NMe-Tyr)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

TMO_c0602: Gln - Gln

Search NORINE for peptide: strict or relaxed

AMP-binding (466..852): Gln

nrpys: Gln

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: glu,gln (Glu, Gln)
Single AA prediction:: gln (Gln)

Stachelhaus prediction details:

Stachelhaus sequence:: DAWQFGLIDK
Nearest Stachelhaus code(s):: Gln DAWQFGLIDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1513..1896): Gln

nrpys: Gln

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: glu,gln (Glu, Gln)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAWQFGLIDK
Nearest Stachelhaus code(s):: Gln DAWQFGLIDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

TMO_c0603: AHBA

CAL_domain (738..1157): AHBA

minowa: AHBA

Prediction, score:

AHBA: 14.0
fatty_acid: 12.0
shikimic_acid: 0.0
NH2: 0.0
Acetyl-CoA: 0.0

TMO_c0604: Pro

Search NORINE for peptide: strict or relaxed

AMP-binding (523..911): Pro

nrpys: Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: pro (Pro)
Single AA prediction:: pro (Pro)

Stachelhaus prediction details:

Stachelhaus sequence:: DVQFAAQVVK
Nearest Stachelhaus code(s):: Pro DVQFAAQVVK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

TMO_c0605: Leu - Thr - Ile

Search NORINE for peptide: strict or relaxed

AMP-binding (249..642): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: leu (Leu)

Stachelhaus prediction details:

Stachelhaus sequence:: DAWFLGHVVK
Nearest Stachelhaus code(s):: Leu DAWFLGHVVK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2212..2615): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNIGMVHK
Nearest Stachelhaus code(s):: Thr DFWNIGMVHK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (3246..3653): Ile

nrpys: Ile

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAFFLGITFK
Nearest Stachelhaus code(s):: Ile DAFFLGITFK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

TMO_c0607: shikimic_acid

CAL_domain (554..969): shikimic_acid

minowa: shikimic_acid

Prediction, score:

shikimic_acid: 0.0
fatty_acid: 0.0
NH2: 0.0
Acetyl-CoA: 0.0
AHBA: 0.0

TMO_c0609: Leu

Search NORINE for peptide: strict or relaxed

AMP-binding (503..896): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: leu (Leu)

Stachelhaus prediction details:

Stachelhaus sequence:: DAWFLGNVVK
Nearest Stachelhaus code(s):: Leu DAWFLGNVVK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

TMO_c0610: Pro

Search NORINE for peptide: strict or relaxed

AMP-binding (257..645): Pro

nrpys: Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: pro (Pro)
Single AA prediction:: pro (Pro)

Stachelhaus prediction details:

Stachelhaus sequence:: DVQFAAQVVK
Nearest Stachelhaus code(s):: Pro DVQFAAQVVK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

TMO_c0611: Tyr

Search NORINE for peptide: strict or relaxed

AMP-binding (525..906): Tyr

nrpys: Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Polar aromatic ring (Tyr, bOH-Tyr)
Single AA prediction:: tyr (Tyr)

Stachelhaus prediction details:

Stachelhaus sequence:: DASTLAAVCK
Nearest Stachelhaus code(s):: Tyr DASTLAAVCK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

Link to PARAS substrate predictor