BGC0000996: frontalamide B biosynthetic gene cluster from Streptomyces sp. SPB78
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Location: 4,892,742 - 4,909,316 nt. (total: 16,575 nt).
This entry is originally from NCBI GenBank GG657742.1.

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
TTA codons
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Gene details
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General
Compounds
Genes
Biosynthesis
Operons
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0000996
Short description frontalamide B biosynthetic gene cluster from Streptomyces sp. SPB78
Status Quality: questionable
The quality level of this entry.

Status: active
The status of this entry.

Completeness: partial
Whether the entry covers everything needed for the pathway producing the compound(s)
Remarks "The ftdB gene is present in three parts, and must be merged. A new paper includes the publication of a fragment that closed a genome sequencing gap within FtdB. Locus tags and protein ID comprising FtdB from N-C terminus: SSLG_04251, protein_id=ADJ54381.1,SSLG_04252. This is why there are two loci listed, which is technically incorrect. Only one locus but in GenBank as the sum of two accession numbers."
Biosynthetic class(es)
  • NRPS (Type I)
  • PKS (Iterative type I)
Loci
GG657742.1
4892742 - 4909316
via Knock-out studies
Compounds
  • frontalamide B
  • frontalamide A
Species Streptomyces sp. SPB78 [taxonomy]
References
Chemical products information
frontalamide B Evidence:
Copy SMILES
C29H36N2O6
Chemical database entries
PubCHEM
frontalamide A Evidence:
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C29H36N2O7
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • SSLG_04250
  • EFL02192.1
 4892742 - 4893686 (+) frontalamide hydroxylase
  • Tailoring
  • Knock-out Activity assay
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  • SSLG_04251
  • EFL02193.1
 4893825 - >4902278 (+) amino acid adenylation domain-containing protein
  • Scaffold biosynthesis
  • Knock-out Activity assay
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  • SSLG_04252
  • EFL02194.1
 4903098 - 4903244 (+) predicted protein
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    • SSLG_04253
    • EFL02195.1
    		 4903376 - 4905091 (+) FAD dependent oxidoreductase
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      • SSLG_04254
      • EFL02196.1
      		 4905267 - 4906997 (+) FAD dependent oxidoreductase
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        • SSLG_04255
        • EFL02197.1
        		 4906994 - 4908049 (+) leukotriene B4 12-hydroxydehydrogenase/15-oxo-prostaglandin 13-reductase
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          • SSLG_04256
          • EFL02198.1
          		 4908135 - 4909316 (+) cytochrome P450 hydroxylase
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            Biosynthesis information

            Biosynthetic modules

            Name
            1
            Type
            nrps-type1
            Genes
            SSLG_04251
            Substrates
            ornithine (evidence: Structure-based inference)
            Integrated Monomers
            Domains
            condensation, adenylation
            Name
            12
            Type
            pks-modular
            Genes
            SSLG_04251
            Substrates
            Integrated Monomers
            Domains
            acyltransferase, ketosynthase, acyltransferase, ketoreductase, dehydratase
            Operons
            Genes Evidence
            SSLG_04256, SSLG_04255, SSLG_04250, SSLG_04251, SSLG_04254, SSLG_04253
            Annotation changelog

            Entry version: 4

            Date
            Changes
            Submitters
            Reviewers
            2022-10-07
            Update chemical activity to schema version 2.11
            • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
            • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

            Entry version: 3

            Date
            Changes
            Submitters
            Reviewers
            2022-09-15
            Update compound structure (SMILES) for frontalamide B
            • (ID: 5UL74VURKJ25VSPPPO3H2NYB)
            • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
            2022-09-15
            Linked new PubChem id for frontalamide B (pubchem:101515038)
            • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
            • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
            2022-09-15
            Add new compound: frontalamide A
            • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
            • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
            2022-09-15
            Corrected NRP module activity
            • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
            • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
            2022-09-15
            Updated bioactivity data
            • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
            • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
            2022-09-15
            Updated NRP substrate specificities
            • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
            • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

            Entry version: 2

            Date
            Changes
            Submitters
            Reviewers
            2019-10-16
            Migrated from v1.4
            • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
            • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

            Entry version: 1

            Date
            Changes
            Submitters
            Reviewers
            2015-06-12
            Submitted
            • (ID: WZFN67G3RBTZ4SMGZ6P35DBX)
            • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
            Detailed domain annotation
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