BGC0000996: frontalamide B biosynthetic gene cluster from Streptomyces sp. SPB78
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Location: 4,892,742 - 4,909,316 nt. (total: 16,575 nt).
This entry is originally from NCBI GenBank GG657742.1.

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General
Compounds
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Polyketide
NRP
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General information about the BGC
MIBiG accession BGC0000996
Short description frontalamide B biosynthetic gene cluster from Streptomyces sp. SPB78
Status Minimal annotation: no
A minimal annotation only contains information on the BGC loci and one or more linked chemical product(s)

Completeness: incomplete
Whether the loci encodes everything needed for the pathway producing the compound(s)
Remarks "The ftdB gene is present in three parts, and must be merged. A new paper includes the publication of a fragment that closed a genome sequencing gap within FtdB. Locus tags and protein ID comprising FtdB from N-C terminus: SSLG_04251, protein_id=ADJ54381.1,SSLG_04252. This is why there are two loci listed, which is technically incorrect. Only one locus but in GenBank as the sum of two accession numbers."
Biosynthetic class(es)
  • NRP
  • Polyketide (Other)
Loci NCBI GenBank: GG657742.1
Compounds
  • frontalamide B
  • frontalamide A
Species Streptomyces sp. SPB78 [taxonomy]
References
Chemical products information
frontalamide B
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C29H36N2O6
Chemical database entries
PubCHEM
frontalamide A
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C29H36N2O7
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • SSLG_04250
  • EFL02192.1
 4892742 - 4893686 (+) frontalamide hydroxylase
  • Tailoring (Hydroxylation)
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  • SSLG_04251
  • EFL02193.1
 4893825 - >4902278 (+) amino acid adenylation domain-containing protein
  • Scaffold biosynthesis
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  • SSLG_04252
  • EFL02194.1
 4903098 - 4903244 (+) predicted protein
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  • SSLG_04253
  • EFL02195.1
 4903376 - 4905091 (+) FAD dependent oxidoreductase
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  • SSLG_04254
  • EFL02196.1
 4905267 - 4906997 (+) FAD dependent oxidoreductase
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  • SSLG_04255
  • EFL02197.1
 4906994 - 4908049 (+) leukotriene B4 12-hydroxydehydrogenase/15-oxo-prostaglandin 13-reductase
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  • SSLG_04256
  • EFL02198.1
 4908135 - 4909316 (+) cytochrome P450 hydroxylase
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Polyketide-specific information
Subclass Other
Cyclic? yes
Polyketide-synthases
Genes Properties Modules
SSLG_04251 Synthase subclass: Iterative type I
Iterative PKS type: Partially reducing, Unknown
Iteration counts: 12
Module 12
Specificity: Malonyl-CoA
Genes: SSLG_04251
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Dehydratase
KR-domain stereochemistry: Unknown
Non-canonical activity:
  • Iterated
Evidence for non-canonical activity:
  • Sequence-based prediction
NRP-specific information
Subclass N/A
Cyclic? yes
Thioesterase genes
  • SSLG_04251 (Type I)
NRP-synthases
Gene Modules
SSLG_04251
Module 1
Specificity: ornithine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: Unknown
Annotation changelog
MIBiG version Submitter Notes
1.0
  • Hidden contributor (ID: WZFN67G3RBTZ4SMGZ6P35DBX, no GDPR consent given).
  • Submitted
2.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Migrated from v1.4
3.0
  • Hidden contributor (ID: 5UL74VURKJ25VSPPPO3H2NYB, no GDPR consent given).
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Update compound structure (SMILES) for frontalamide B
  • Linked new PubChem id for frontalamide B (pubchem:101515038)
  • Add new compound: frontalamide A
  • Corrected NRP module activity
  • Updated bioactivity data
  • Updated NRP substrate specificities
3.1
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Update chemical activity to schema version 2.11
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