BGC0000999 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000999 (Lysobacter enzymogenes)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS,NRPS	1	20,280	heat-stable antifungal factor	NRPS:Type I+PKS	100%

BGC0000999 - Region 1 - NRPS,T1PKS

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Location: 1 - 20,280 nt. (total: 20,280 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: cds(Condensation and (AMP-binding or A-OX))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

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TTA codons

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 20,281
Label		BGC0000999

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000999	2.00	NRP, Polyketide	heat-stable antifungal factor	Lysobacter enzymogenes
BGC0002365	0.72	Other, Polyketide	10-epi-HSAF, 10-epi-3-deOH-HSAF, 10-epi-maltophilin, 10-epi-xanthobaccin C, 10-epi-hydroxymaltophilin, 10-epi-FI-2	Streptomyces sp.
BGC0001043	0.72	NRP, Polyketide	SGR PTMs, SGR PTM Compound b, SGR PTM Compound c, SGR PTM Compound d	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001726	0.58	NRP, Polyketide	pactamides	Streptomyces pactum
BGC0000996	0.53	NRP, Polyketide	frontalamide B, frontalamide A	Streptomyces sp. SPB78
BGC0001556	0.49	NRP, Polyketide	combamide	Streptomyces sp.
BGC0001132	0.48	NRP	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001833	0.48	NRP	icosalide A, icosalide B	Burkholderia gladioli
BGC0001825	0.47	NRP	xenematide	Xenorhabdus nematophila AN6/1
BGC0002528	0.47	NRP	aminochelin, azotochelin, protochelin	Azotobacter vinelandii CA

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000999	1.00	NRP, Polyketide	heat-stable antifungal factor	Lysobacter enzymogenes
BGC0002365	0.69	Other, Polyketide	10-epi-HSAF, 10-epi-3-deOH-HSAF, 10-epi-maltophilin, 10-epi-xanthobaccin C, 10-epi-hydroxymaltophilin, 10-epi-FI-2	Streptomyces sp.
BGC0001043	0.69	NRP, Polyketide	SGR PTMs, SGR PTM Compound b, SGR PTM Compound c, SGR PTM Compound d	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001726	0.63	NRP, Polyketide	pactamides	Streptomyces pactum
BGC0000996	0.60	NRP, Polyketide	frontalamide B, frontalamide A	Streptomyces sp. SPB78
BGC0001556	0.58	NRP, Polyketide	combamide	Streptomyces sp.
BGC0001132	0.58	NRP	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001833	0.57	NRP	icosalide A, icosalide B	Burkholderia gladioli
BGC0001825	0.57	NRP	xenematide	Xenorhabdus nematophila AN6/1
BGC0002528	0.57	NRP	aminochelin, azotochelin, protochelin	Azotobacter vinelandii CA

Similar known gene clusters from MIBiG 4.0

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 20280:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk - Orn)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ABL86391.1

Search NORINE for peptide: strict or relaxed

PKS_AT (544..840): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 66.7%; Methylmalonyl-CoA: 58.3%; CE-Malonyl-CoA: 54.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 114.3
Methoxymalonyl-CoA: 108.0
Malonyl-CoA: 107.7
Isobutyryl-CoA: 95.1
Ethylmalonyl-CoA: 92.3
2-Methylbutyryl-CoA: 68.1
Propionyl-CoA: 66.4
Acetyl-CoA: 57.4
trans-1,2-CPDA: 54.6
Benzoyl-CoA: 52.5
3-Methylbutyryl-CoA: 42.2
inactive: 38.0
fatty_acid: 37.3
CHC-CoA: 32.7

AMP-binding (2296..2707): Orn

nrpys: Orn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Long positively charged side chain (Orn, Lys, Arg)
Small clusters prediction:: Orn and hydroxy-Orn specific (Orn, OH-Orn)
Single AA prediction:: orn (Orn)

Stachelhaus prediction details:

Stachelhaus sequence:: DVGEVGSIDK
Nearest Stachelhaus code(s):: Orn DVGEVGSIDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)