BGC0001000 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001000 (Lyngbya majuscula)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	NRPS,T1PKS	1	41,964	hectochlorin	NRPS:Type I+PKS:Type I	100%

BGC0001000 - Region 1 - NRPS,T1PKS

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Location: 1 - 41,964 nt. (total: 41,964 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 41,965
Label		BGC0001000

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001000	1.63	NRP, Polyketide	hectochlorin	Lyngbya majuscula
BGC0002405	0.61	Saccharide	mid-chain acyl sugars	Solanum lycopersicum
BGC0001161	0.51	Polyketide	1-heptadecene	Moorea bouillonii PNG
BGC0001163	0.51	Polyketide	1-heptadecene	Moorea producens 3L
BGC0001162	0.49	Polyketide	1-heptadecene	Moorea producens JHB
BGC0001219	0.49	Polyketide	shanorellin	Chaetomium globosum CBS 148.51
BGC0001834	0.45	NRP	argyrin	Cystobacter sp. SBCb004
BGC0001160	0.45	Polyketide	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001164	0.45	Polyketide	1-heptadecene	Cyanothece sp. PCC 7822
BGC0000979	0.43	NRP, Polyketide	cylindrospermopsin	Aphanizomenon sp. 10E9

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001000	1.00	NRP, Polyketide	hectochlorin	Lyngbya majuscula
BGC0000677	0.64	Terpene	cyclooctatin	Streptomyces melanosporofaciens
BGC0002405	0.64	Saccharide	mid-chain acyl sugars	Solanum lycopersicum
BGC0002219	0.64	Terpene	(-)-(R)-nephthenol	Stereum hirsutum FP-91666 SS1
BGC0001219	0.61	Polyketide	shanorellin	Chaetomium globosum CBS 148.51
BGC0001161	0.60	Polyketide	1-heptadecene	Moorea bouillonii PNG
BGC0001163	0.60	Polyketide	1-heptadecene	Moorea producens 3L
BGC0001264	0.59	Polyketide	betaenone A, betaenone B, betaenone C	Phoma betae
BGC0001162	0.59	Polyketide	1-heptadecene	Moorea producens JHB
BGC0002678	0.58	NRP	pyochelin	Burkholderia cepacia ATCC 25416

Similar known gene clusters from MIBiG 4.0

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 41964:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-ohmal) + (Cys) + (Cys)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

AAY42393.1

CAL_domain (167..488): pk

minowa: NH2

Prediction, score:

NH2: 149.6
Acetyl-CoA: 58.1
fatty_acid: 37.5
AHBA: 19.1
shikimic_acid: 0.0

AAY42396.1

PKS_AT (529..832): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 95.8%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 197.9
inactive: 175.4
Methoxymalonyl-CoA: 65.2
Methylmalonyl-CoA: 61.3
Isobutyryl-CoA: 38.4
Propionyl-CoA: 32.8
Ethylmalonyl-CoA: 24.2
2-Methylbutyryl-CoA: 19.1
CHC-CoA: 16.4
Acetyl-CoA: 16.4
Benzoyl-CoA: 15.5
trans-1,2-CPDA: 13.6
3-Methylbutyryl-CoA: 10.6
fatty_acid: 0.0

AAY42397.1

Search NORINE for peptide: strict or relaxed

CAL_domain (850..1267): pk

minowa: fatty_acid

Prediction, score:

fatty_acid: 51.8
NH2: 10.8
Acetyl-CoA: 8.5
shikimic_acid: 0.0
AHBA: -2.8

AMP-binding (2502..2902): Cys

nrpys: Cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: DLYNLSLIWK
Nearest Stachelhaus code(s):: Cys DLYNLSLIWK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AAY42398.1

Search NORINE for peptide: strict or relaxed

AMP-binding (542..926): X

nrpys: Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: ESWNLSAGEK
Nearest Stachelhaus code(s):: Hiv DTWGLSLGEK (59% 8Å match)
Stachelhaus code match:: 60% (weak)

CAL_domain (1142..1560): pk

minowa: fatty_acid

Prediction, score:

fatty_acid: 51.8
NH2: 10.8
Acetyl-CoA: 8.5
shikimic_acid: 0.0
AHBA: -2.8

AMP-binding (2796..3198): Cys

nrpys: Cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: DLYNLSLIWK
Nearest Stachelhaus code(s):: Cys DLYNLSLIWK (100% 8Å match)
Stachelhaus code match:: 100% (strong)