BGC0001003 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001003 (Kitasatospora aureofaciens)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS,NRPS	1	66,871	High	α-lipomycin	NRPS:Type I+PKS:Type I+saccharide:hybrid/tailoring

BGC0001003 - Region 1 - NRPS,T1PKS

Download region GenBank file

Download region SVG

Location: 1 - 66,871 nt. (total: 66,871 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 66,872
Label		BGC0001003

Tailoring functions

Oxidoreductases: oxidoreductase

lipDig3: aldo/keto reductase family oxidoreductase

smcogs: SMCOG1039: aldo/keto reductase family oxidoreductase

Transferases: glycosyltransferase methyltransferase transferase

lipDig1: glucose-1-phosphate adenylyl/thymidylyltransferase

smcogs: SMCOG1064: glucose-1-phosphate adenylyl/thymidylyltransferase

lipGtf: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

lipMt: O-methyltransferase

smcogs: SMCOG1042: O-methyltransferase

Hydrolases: esterase hydrolase

lipTe: thioesterase

smcogs: SMCOG1004: thioesterase

lipX4: beta-lactamase

smcogs: SMCOG1053: beta-lactamase

Lyases: dehydratase

lipDig2: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

lipDig4: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Ligases: ligase synthase

lipNrps: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

lipPks1: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

lipPks2: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

lipPks3: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

lipPks4: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001003.5	1.59	NRPS, PKS, saccharide	α-lipomycin	Kitasatospora aureofaciens
BGC0001276.3	0.90	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273.3	0.81	PKS	asperlactone	Aspergillus ochraceus
BGC0001244.3	0.80	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001268.4	0.67	NRPS, PKS	fusarin C	Fusarium fujikuroi
BGC0002192.3	0.67	PKS	FR901512	fungal sp. No.14919
BGC0000041.3	0.67	PKS	oronofacic acid	Pseudomonas syringae
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001284.4	0.65	PKS	alternariol	Parastagonospora nodorum SN15
BGC0002154.2	0.62	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001003.5	1.00	NRPS, PKS, saccharide	α-lipomycin	Kitasatospora aureofaciens
BGC0001273.3	0.77	PKS	asperlactone	Aspergillus ochraceus
BGC0001244.3	0.76	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001276.3	0.75	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001268.4	0.67	NRPS, PKS	fusarin C	Fusarium fujikuroi
BGC0002192.3	0.67	PKS	FR901512	fungal sp. No.14919
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0002154.2	0.66	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001257.3	0.66	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0000107.3	0.66	PKS	naphthopyrone	Aspergillus nidulans FGSC A4

Similar known clusters from MIBiG 4.0

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 66871:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (pk - Me-ohmal) + (Me-ccmal - ccmal) + (ccmal - ccmal) + (ccmal - mal)
Non-colinear ordering:: lipPks1 + lipPks2 + lipPks3 + lipPks4

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 0 - 36851:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (pk - Me-ohmal) + (Me-ccmal - ccmal) + (ccmal - ccmal)
Non-colinear ordering:: lipPks1 + lipPks2 + lipPks3

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

lipPks1: pk - mmal

PKS_AT (172..470): pk

ATSignature: Isobutyryl-CoA

Top 3 matches:: Isobutyryl-CoA: 100.0%; Methylmalonyl-CoA: 70.8%; 2-Methylbutyryl-CoA: 70.8%

minowa: 2-Methylbutyryl-CoA

Prediction, score:

2-Methylbutyryl-CoA: 163.7
Methylmalonyl-CoA: 155.0
Ethylmalonyl-CoA: 144.5
Propionyl-CoA: 128.5
Methoxymalonyl-CoA: 126.3
Malonyl-CoA: 107.3
CHC-CoA: 97.6
trans-1,2-CPDA: 81.4
Isobutyryl-CoA: 76.2
Benzoyl-CoA: 55.7
3-Methylbutyryl-CoA: 37.6
Acetyl-CoA: 37.4
inactive: 33.3
fatty_acid: 26.3

PKS_AT (1178..1475): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 213.5
Ethylmalonyl-CoA: 208.2
Methoxymalonyl-CoA: 175.6
2-Methylbutyryl-CoA: 152.0
trans-1,2-CPDA: 125.5
Propionyl-CoA: 106.7
Malonyl-CoA: 96.3
CHC-CoA: 94.2
Isobutyryl-CoA: 84.3
Benzoyl-CoA: 51.8
Acetyl-CoA: 35.8
inactive: 25.3
fatty_acid: 20.8
3-Methylbutyryl-CoA: 14.9

lipPks2: mmal - mal

PKS_AT (575..872): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 213.5
Ethylmalonyl-CoA: 208.2
Methoxymalonyl-CoA: 175.6
2-Methylbutyryl-CoA: 152.0
trans-1,2-CPDA: 125.5
Propionyl-CoA: 106.7
Malonyl-CoA: 96.3
CHC-CoA: 94.2
Isobutyryl-CoA: 84.3
Benzoyl-CoA: 51.8
Acetyl-CoA: 35.8
inactive: 25.3
fatty_acid: 20.8
3-Methylbutyryl-CoA: 14.9

PKS_AT (2321..2611): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 75.0%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 203.5
inactive: 86.2
Methylmalonyl-CoA: 69.3
Methoxymalonyl-CoA: 68.0
Propionyl-CoA: 35.4
Ethylmalonyl-CoA: 24.9
Isobutyryl-CoA: 23.4
Acetyl-CoA: 19.1
2-Methylbutyryl-CoA: 17.6
Benzoyl-CoA: 17.3
trans-1,2-CPDA: 15.3
CHC-CoA: 14.8
fatty_acid: 14.4
3-Methylbutyryl-CoA: 0.0

lipPks3: mal - mal

PKS_AT (582..870): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 75.0%; Propionyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 173.9
inactive: 75.6
Methylmalonyl-CoA: 62.0
Methoxymalonyl-CoA: 58.4
Isobutyryl-CoA: 38.1
Acetyl-CoA: 24.8
Propionyl-CoA: 22.6
Benzoyl-CoA: 15.2
2-Methylbutyryl-CoA: 13.7
trans-1,2-CPDA: 11.5
CHC-CoA: 5.8
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0
Ethylmalonyl-CoA: -2.2

PKS_AT (2353..2643): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 75.0%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 200.7
inactive: 81.9
Methylmalonyl-CoA: 67.2
Methoxymalonyl-CoA: 65.7
Propionyl-CoA: 31.6
Isobutyryl-CoA: 21.3
Ethylmalonyl-CoA: 21.1
Acetyl-CoA: 16.3
CHC-CoA: 15.8
Benzoyl-CoA: 15.6
fatty_acid: 14.9
trans-1,2-CPDA: 14.6
2-Methylbutyryl-CoA: 14.0
3-Methylbutyryl-CoA: 0.0

lipPks4: mal - mal

PKS_AT (560..850): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 75.0%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 204.2
inactive: 89.4
Methylmalonyl-CoA: 69.9
Methoxymalonyl-CoA: 67.0
Propionyl-CoA: 35.4
Isobutyryl-CoA: 26.7
Ethylmalonyl-CoA: 20.7
Acetyl-CoA: 19.6
2-Methylbutyryl-CoA: 18.6
Benzoyl-CoA: 17.3
trans-1,2-CPDA: 15.1
CHC-CoA: 14.8
fatty_acid: 14.5
3-Methylbutyryl-CoA: 6.8

PKS_AT (2293..2586): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 75.0%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 202.5
inactive: 85.2
Methylmalonyl-CoA: 68.3
Methoxymalonyl-CoA: 65.0
Propionyl-CoA: 32.7
Isobutyryl-CoA: 25.7
Ethylmalonyl-CoA: 20.5
Benzoyl-CoA: 17.3
2-Methylbutyryl-CoA: 17.0
Acetyl-CoA: 16.3
CHC-CoA: 14.8
trans-1,2-CPDA: 14.7
fatty_acid: 14.5
3-Methylbutyryl-CoA: 0.0

Link to PARAS substrate predictor