BGC0001003 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001003 (Kitasatospora aureofaciens)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS,NRPS	1	66,871	α-lipomycin	NRPS:Type I+PKS:Type I+saccharide:hybrid/tailoring	100%

BGC0001003 - Region 1 - NRPS,T1PKS

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Location: 1 - 66,871 nt. (total: 66,871 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: cds(Condensation and (AMP-binding or A-OX))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 66,872
Label		BGC0001003

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001003	1.58	NRP, Polyketide, Saccharide	α-lipomycin	Kitasatospora aureofaciens
BGC0000041	0.67	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0001163	0.60	Polyketide	1-heptadecene	Moorea producens 3L
BGC0001276	0.58	Polyketide	6-methylsalicyclic acid	Aspergillus terreus
BGC0001164	0.58	Polyketide	1-heptadecene	Cyanothece sp. PCC 7822
BGC0000813	0.57	Alkaloid	K-252a	Nonomuraea longicatena
BGC0000353	0.55	NRP	tacrolimus	Streptomyces sp. MA6548
BGC0000160	0.52	Polyketide	terreic acid	Aspergillus terreus NIH2624
BGC0002171	0.51	NRP, Polyketide	pyrophen, campyrone B	Aspergillus niger ATCC 1015
BGC0002275	0.51	NRP, Polyketide	pyrophen, campyrone B	Aspergillus niger CBS 101883

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001003	1.00	NRP, Polyketide, Saccharide	α-lipomycin	Kitasatospora aureofaciens
BGC0000016	0.71	Other	amphotericin B	Streptomyces nodosus
BGC0000813	0.68	Alkaloid	K-252a	Nonomuraea longicatena
BGC0000278	0.67	Polyketide	urdamycin	Streptomyces fradiae
BGC0002435	0.67	Other	coformycin, aristeromycin	Micromonospora haikouensis
BGC0001273	0.65	Polyketide	asperlactone	Aspergillus ochraceus
BGC0000996	0.64	NRP, Polyketide	frontalamide B, frontalamide A	Streptomyces sp. SPB78
BGC0001244	0.64	Polyketide	(-)-Mellein	Parastagonospora nodorum
BGC0001163	0.64	Polyketide	1-heptadecene	Moorea producens 3L
BGC0000041	0.64	Polyketide	oronofacic acid	Pseudomonas syringae

Similar known gene clusters from MIBiG 4.0

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 66871:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (pk - Me-ohmal) + (Me-ccmal - ccmal) + (ccmal - ccmal) + (ccmal - mal)
Non-colinear ordering:: lipPks1 + lipPks2 + lipPks3 + lipPks4

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 0 - 36851:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (pk - Me-ohmal) + (Me-ccmal - ccmal) + (ccmal - ccmal)
Non-colinear ordering:: lipPks1 + lipPks2 + lipPks3

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

lipPks1

PKS_AT (172..470): pk

ATSignature: Isobutyryl-CoA

Top 3 matches:: Isobutyryl-CoA: 100.0%; Methylmalonyl-CoA: 70.8%; 2-Methylbutyryl-CoA: 70.8%

minowa: 2-Methylbutyryl-CoA

Prediction, score:

2-Methylbutyryl-CoA: 163.7
Methylmalonyl-CoA: 155.0
Ethylmalonyl-CoA: 144.5
Propionyl-CoA: 128.5
Methoxymalonyl-CoA: 126.3
Malonyl-CoA: 107.3
CHC-CoA: 97.6
trans-1,2-CPDA: 81.4
Isobutyryl-CoA: 76.2
Benzoyl-CoA: 55.7
3-Methylbutyryl-CoA: 37.6
Acetyl-CoA: 37.4
inactive: 33.3
fatty_acid: 26.3

PKS_AT (1178..1475): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 213.5
Ethylmalonyl-CoA: 208.2
Methoxymalonyl-CoA: 175.6
2-Methylbutyryl-CoA: 152.0
trans-1,2-CPDA: 125.5
Propionyl-CoA: 106.7
Malonyl-CoA: 96.3
CHC-CoA: 94.2
Isobutyryl-CoA: 84.3
Benzoyl-CoA: 51.8
Acetyl-CoA: 35.8
inactive: 25.3
fatty_acid: 20.8
3-Methylbutyryl-CoA: 14.9

lipPks2

PKS_AT (575..872): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 213.5
Ethylmalonyl-CoA: 208.2
Methoxymalonyl-CoA: 175.6
2-Methylbutyryl-CoA: 152.0
trans-1,2-CPDA: 125.5
Propionyl-CoA: 106.7
Malonyl-CoA: 96.3
CHC-CoA: 94.2
Isobutyryl-CoA: 84.3
Benzoyl-CoA: 51.8
Acetyl-CoA: 35.8
inactive: 25.3
fatty_acid: 20.8
3-Methylbutyryl-CoA: 14.9

PKS_AT (2321..2611): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 75.0%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 203.5
inactive: 86.2
Methylmalonyl-CoA: 69.3
Methoxymalonyl-CoA: 68.0
Propionyl-CoA: 35.4
Ethylmalonyl-CoA: 24.9
Isobutyryl-CoA: 23.4
Acetyl-CoA: 19.1
2-Methylbutyryl-CoA: 17.6
Benzoyl-CoA: 17.3
trans-1,2-CPDA: 15.3
CHC-CoA: 14.8
fatty_acid: 14.4
3-Methylbutyryl-CoA: 0.0

lipPks3

PKS_AT (582..870): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 75.0%; Propionyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 173.9
inactive: 75.6
Methylmalonyl-CoA: 62.0
Methoxymalonyl-CoA: 58.4
Isobutyryl-CoA: 38.1
Acetyl-CoA: 24.8
Propionyl-CoA: 22.6
Benzoyl-CoA: 15.2
2-Methylbutyryl-CoA: 13.7
trans-1,2-CPDA: 11.5
CHC-CoA: 5.8
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0
Ethylmalonyl-CoA: -2.2

PKS_AT (2353..2643): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 75.0%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 200.7
inactive: 81.9
Methylmalonyl-CoA: 67.2
Methoxymalonyl-CoA: 65.7
Propionyl-CoA: 31.6
Isobutyryl-CoA: 21.3
Ethylmalonyl-CoA: 21.1
Acetyl-CoA: 16.3
CHC-CoA: 15.8
Benzoyl-CoA: 15.6
fatty_acid: 14.9
trans-1,2-CPDA: 14.6
2-Methylbutyryl-CoA: 14.0
3-Methylbutyryl-CoA: 0.0

lipPks4

PKS_AT (560..850): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 75.0%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 204.2
inactive: 89.4
Methylmalonyl-CoA: 69.9
Methoxymalonyl-CoA: 67.0
Propionyl-CoA: 35.4
Isobutyryl-CoA: 26.7
Ethylmalonyl-CoA: 20.7
Acetyl-CoA: 19.6
2-Methylbutyryl-CoA: 18.6
Benzoyl-CoA: 17.3
trans-1,2-CPDA: 15.1
CHC-CoA: 14.8
fatty_acid: 14.5
3-Methylbutyryl-CoA: 6.8

PKS_AT (2293..2586): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 75.0%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 202.5
inactive: 85.2
Methylmalonyl-CoA: 68.3
Methoxymalonyl-CoA: 65.0
Propionyl-CoA: 32.7
Isobutyryl-CoA: 25.7
Ethylmalonyl-CoA: 20.5
Benzoyl-CoA: 17.3
2-Methylbutyryl-CoA: 17.0
Acetyl-CoA: 16.3
CHC-CoA: 14.8
trans-1,2-CPDA: 14.7
fatty_acid: 14.5
3-Methylbutyryl-CoA: 0.0