Genomic loci for this biosynthetic pathway

Cluster Type From To
The following clusters are from record BGC0001003.1:
Cluster 1NRP / Polyketide / Saccharide166871

BGC0001003, lipomycin biosynthetic gene cluster from Streptomyces aureofaciens. Locus 1. Full MIBiG entry.

Chemical compounds

Compound: alpha-lipomycin
ChEBI ID: 80123
SMILES string: Copy to clipboard
Molecular formula:
Average molecular mass: Da
Molecular activity: Antibacterial

Class-specific details

Biosynthetic class(es):
NRP / Polyketide / Saccharide
Nonribosomal peptide subclass:
Other lipopeptide (linear)
pentaen chain
Thioesterase type:
Type II
Release / cyclization type:
Unknown

Nonribosomal peptide synthetases:
lipNrps (ABB05101.1)
Module 1
A specificity: Glutamate
Evidence for specificity: Structure-based inference
C domain subtype: Unknown
Polyketide subclass:
Polyene (linear)
Polyketide synthase subclass:
Modular type I
Starter unit:
Isobutyryl-CoA
Thioesterase type:
None
Release / cyclization type:
None

Modular polyketide synthases:
lipPks1 (ABB05102.1)
Module 0
AT specificity: Isobutyryl-CoA
Evidence for specificity: Activity assay
Module 1
AT specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: Unknown

lipPks2 (ABB05103.1)
Module 2
AT specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: Unknown
Module 3
AT specificity: Malonyl-CoA
Evidence for specificity: Unknown

lipPks3 (ABB05104.1)
Module 4
AT specificity: Malonyl-CoA
Evidence for specificity: Unknown
Module 5
AT specificity: Malonyl-CoA
Evidence for specificity: Unknown

lipPks4 (ABB05105.1)
Module 6
AT specificity: Malonyl-CoA
Evidence for specificity: Unknown
Module 7
AT specificity: Malonyl-CoA
Evidence for specificity: Unknown

Saccharide subclass:
hybrid/tailoring
Glycosyltransferases:
ABB05096.1:
Specificity: L-digitoxose
Evidence for specificity: Unknown
Sugar biosynthesis subcluster: ABB05097.1, ABB05098.1, ABB05109.1, ABB05110.1,ABB05111.1

Gene cluster description

lipomycin (BGC0001003). Gene Cluster 1. Biosynthetic class = NRP/Polyketide/Saccharide. GenBank DQ176871. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Domain annotation

Homologous known gene clusters

General MIBiG information on this cluster

Complete gene cluster sequence?complete
Evidence for cluster-compound connection:Knock-out studies
MIxS-compliance:Unknown
Contact for this cluster:Andreas Bechthold (Albert-Ludwigs-Universitaet Freiburg)

Literature references

1. Bihlmaier C et al. (2006) Biosynthetic gene cluster for the polyenoyltetramic acid alpha-lipomycin. Antimicrob Agents Chemother 50(6):2113-21. doi: 10.1128/AAC.00007-06.
2. Horbal L et al. (2010) Characterization and analysis of the regulatory network involved in control of lipomycin biosynthesis in Streptomyces aureofaciens Tu117. Appl Microbiol Biotechnol 85(4):1069-79. doi:
3. Yuzawa S et al. (2013) Broad substrate specificity of the loading didomain of the lipomycin polyketide synthase. Biochemistry 52(22):3791-3. doi: 10.1021/bi400520t. Epub 2013 May 23.
4. Yuzawa S et al. (2014) Enzyme analysis of the polyketide synthase leads to the discovery of a novel analog of the antibiotic alpha-lipomycin. J Antibiot (Tokyo) 67(2):199-201. doi: 10.1038/ja.2013.110. Epub 2013
5. Kunze B et al. (1972 TI) [Metabolic products of microorganisms. 3 lipomycins. I. Isolation, characterization and first studies of the structure and the mechanism of action]. Arch Mikrobiol 86(2):147-74.