BGC0001008 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001008 (Actinomadura madurae)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS,NRPS	1	62,812	maduropeptin	PKS:Iterative type I	100%

BGC0001008 - Region 1 - NRPS,T1PKS

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Location: 1 - 62,812 nt. (total: 62,812 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: cds(Condensation and (AMP-binding or A-OX))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

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TTA codons

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 62,813
Label		BGC0001008

Detailed domain annotation

Selected features only

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Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001008	1.67	Polyketide	maduropeptin	Actinomadura madurae
BGC0002341	1.04	Other	3,5-dibromo-p-anisic acid	Planctomycetales bacterium 10988
BGC0002608	0.96	NRP, Polyketide	ochratoxin A	Aspergillus steynii
BGC0000056	0.74	Polyketide	esperamicin	Actinomadura verrucosospora
BGC0002438	0.70	Alkaloid	sporidesmin A	Pithomyces chartarum
BGC0002711	0.68	Polyketide	nostovalerolactone, 9-dehydronostovalerolactone	Nostoc punctiforme PCC 73102
BGC0000343	0.67	NRP	enterobactin	Pseudomonas sp. J465
BGC0001335	0.67	Alkaloid	borregomycin A, borregomycin B, borregomycin C, borregomycin D	uncultured bacterium
BGC0001068	0.66	Terpene, Polyketide	pyripyropene A	unidentified unclassified sequences.
BGC0002474	0.66	NRP	agrobactin	Agrobacterium tumefaciens

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001008	1.00	Polyketide	maduropeptin	Actinomadura madurae
BGC0000056	0.70	Polyketide	esperamicin	Actinomadura verrucosospora
BGC0000343	0.69	NRP	enterobactin	Pseudomonas sp. J465
BGC0002493	0.69	NRP	parabactin	Paracoccus denitrificans PD1222
BGC0002474	0.68	NRP	agrobactin	Agrobacterium tumefaciens
BGC0002341	0.68	Other	3,5-dibromo-p-anisic acid	Planctomycetales bacterium 10988
BGC0000677	0.68	Terpene	cyclooctatin	Streptomyces melanosporofaciens
BGC0002229	0.67	Polyketide	6-MSA, aculin A	Aspergillus aculeatus ATCC 16872
BGC0002406	0.67	Other	hydroxycinnamoyltyramine	Oryza sativa Japonica Group
BGC0001925	0.66	Alkaloid	altemicidin, SB-203207, SB-203208	Streptomyces sp.

Similar known gene clusters from MIBiG 4.0

Module view

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 62812:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-ccmal) + (X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 0 - 38586:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-ccmal) + (X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 17886 - 61234:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-ccmal) + (X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 4, location 31715 - 62812:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

mdpB

PKS_AT (548..840): mmal

ATSignature: Methylmalonyl-CoA, Malonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 79.2%; Malonyl-CoA: 79.2%; Ethylmalonyl-CoA: 75.0%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 131.3
Methoxymalonyl-CoA: 115.0
Malonyl-CoA: 111.1
Ethylmalonyl-CoA: 106.3
Isobutyryl-CoA: 77.4
3-Methylbutyryl-CoA: 61.7
Propionyl-CoA: 59.9
2-Methylbutyryl-CoA: 56.2
Acetyl-CoA: 51.7
Benzoyl-CoA: 48.4
trans-1,2-CPDA: 44.2
inactive: 36.5
CHC-CoA: 35.2
fatty_acid: 19.0

mdpB2

AMP-binding (33..448): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Small clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Single AA prediction:: dhb (diOH-Bz)

Stachelhaus prediction details:

Stachelhaus sequence:: PLPSHGVICK
Nearest Stachelhaus code(s):: meSal PLPSHGVVCK (88% 8Å match)
Stachelhaus code match:: 90% (moderate)

mdpC1

AMP-binding (504..910): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DPCQVMVIAK
Nearest Stachelhaus code(s):: bTyr or bTyr DPAQLMLIAK (79% 8Å match)
Stachelhaus code match:: 70% (weak)

mdpE

PKS_AT (618..848): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; inactive: 58.3%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 70.9
Methoxymalonyl-CoA: 42.8
Methylmalonyl-CoA: 39.0
inactive: 31.6
Isobutyryl-CoA: 19.4
fatty_acid: 13.7
Ethylmalonyl-CoA: 10.6
2-Methylbutyryl-CoA: 9.8
Benzoyl-CoA: 9.0
trans-1,2-CPDA: 8.5
CHC-CoA: 3.5
Acetyl-CoA: 1.9
3-Methylbutyryl-CoA: 0.0
Propionyl-CoA: -1.0