BGC0001010 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001010 (Melittangium lichenicola)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	NRPS,T1PKS	1	51,855	High	melithiazol A	NRPS:Type I+PKS:Type I

BGC0001010 - Region 1 - NRPS,T1PKS

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Location: 1 - 51,855 nt. (total: 51,855 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

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TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 51,856
Label		BGC0001010

Tailoring functions

Oxidoreductases: dehydrogenase

CAD89770.1: dehydrogenase

smcogs: SMCOG1222: dehydrogenase

Transferases: acylase

melE: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

Lyases: dehydratase hydratase

CAD89771.1: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

melJ: Nitrilase/cyanide hydratase and apolipoprotein

smcogs: SMCOG1294: Nitrilase/cyanide hydratase and apolipoprotein

Ligases: ligase synthase

CAD89769.1: polyprenol-monophosphomannose synthase ppm1

smcogs: SMCOG1123: polyprenol-monophosphomannose synthase ppm1

melB: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

melC: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

melD: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

melF: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001010.5	1.98	NRPS, PKS	melithiazol A	Melittangium lichenicola
BGC0001024.5	0.93	NRPS, PKS	myxothiazol	Stigmatella aurantiaca DW4/3-1
BGC0000982.5	0.69	NRPS, PKS	cystothiazole A	Cystobacter fuscus
BGC0001268.4	0.68	NRPS, PKS	fusarin C	Fusarium fujikuroi
BGC0001273.3	0.68	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.67	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0002154.2	0.67	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001257.3	0.67	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0001258.3	0.67	PKS	1,3,6,8-tetrahydroxynaphthalene	Glarea lozoyensis
BGC0002191.2	0.67	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001010.5	1.00	NRPS, PKS	melithiazol A	Melittangium lichenicola
BGC0001024.5	0.76	NRPS, PKS	myxothiazol	Stigmatella aurantiaca DW4/3-1
BGC0000982.5	0.68	NRPS, PKS	cystothiazole A	Cystobacter fuscus
BGC0001268.4	0.67	NRPS, PKS	fusarin C	Fusarium fujikuroi
BGC0001273.3	0.67	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.67	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0002154.2	0.67	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001257.3	0.67	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0001258.3	0.67	PKS	1,3,6,8-tetrahydroxynaphthalene	Glarea lozoyensis
BGC0002191.2	0.67	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 51855:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk) + (Cys) + (Cys - ccmal) + (OMe-Me-ohmal) + (OMe-mal) + (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

melB: pk

PKS_AT (553..847): pk

ATSignature: Isobutyryl-CoA

Top 3 matches:: Isobutyryl-CoA: 100.0%; Methylmalonyl-CoA: 70.8%; Malonyl-CoA: 58.3%

minowa: Isobutyryl-CoA

Prediction, score:

Isobutyryl-CoA: 236.4
Methylmalonyl-CoA: 90.5
Methoxymalonyl-CoA: 83.7
Malonyl-CoA: 73.6
Propionyl-CoA: 33.6
Benzoyl-CoA: 30.8
fatty_acid: 27.0
3-Methylbutyryl-CoA: 25.3
inactive: 25.2
Acetyl-CoA: 19.1
2-Methylbutyryl-CoA: 19.0
CHC-CoA: 11.8
trans-1,2-CPDA: 0.0
Ethylmalonyl-CoA: -1.6

melC: Cys

Search NORINE for peptide: strict or relaxed

AMP-binding (517..915): Cys

nrpys: Cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: DLYNMSLVWK
Nearest Stachelhaus code(s):: Cys DLYNMSLVWK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

melD: Cys - mal

Search NORINE for peptide: strict or relaxed

AMP-binding (539..938): Cys

nrpys: Cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: DLYNLSLIWK
Nearest Stachelhaus code(s):: Cys DLYNLSLIWK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

PKS_AT (1979..2271): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 62.5%; Isobutyryl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 146.6
inactive: 109.9
Methoxymalonyl-CoA: 77.3
Methylmalonyl-CoA: 61.4
Propionyl-CoA: 41.2
2-Methylbutyryl-CoA: 30.8
Ethylmalonyl-CoA: 29.8
Isobutyryl-CoA: 25.0
CHC-CoA: 23.7
Benzoyl-CoA: 17.9
trans-1,2-CPDA: 15.2
Acetyl-CoA: 11.6
fatty_acid: 10.0
3-Methylbutyryl-CoA: 0.0

melE: mmal

PKS_AT (571..866): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Malonyl-CoA: 83.3%; Ethylmalonyl-CoA: 79.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 175.2
Ethylmalonyl-CoA: 147.8
Methoxymalonyl-CoA: 133.8
Isobutyryl-CoA: 130.7
Benzoyl-CoA: 123.3
Malonyl-CoA: 99.2
Acetyl-CoA: 95.1
2-Methylbutyryl-CoA: 81.3
Propionyl-CoA: 71.6
trans-1,2-CPDA: 67.5
CHC-CoA: 62.1
3-Methylbutyryl-CoA: 44.0
fatty_acid: 43.0
inactive: 31.0

melF: mal

PKS_AT (569..861): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 62.5%; Isobutyryl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 146.6
inactive: 109.9
Methoxymalonyl-CoA: 77.3
Methylmalonyl-CoA: 61.4
Propionyl-CoA: 41.2
2-Methylbutyryl-CoA: 30.8
Ethylmalonyl-CoA: 29.8
Isobutyryl-CoA: 25.0
CHC-CoA: 23.7
Benzoyl-CoA: 17.9
trans-1,2-CPDA: 15.2
Acetyl-CoA: 11.6
fatty_acid: 10.0
3-Methylbutyryl-CoA: 0.0

melG: X

A-OX (554..1316): X

Link to PARAS substrate predictor