BGC0001012 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001012 (Streptomyces violaceusniger)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS,NRPS	1	91,893

BGC0001012 - Region 1 - NRPS,T1PKS

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Location: 1 - 91,893 nt. (total: 91,893 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 91,894
Label		BGC0001012

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

merC: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

merD: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

Ligases: ligase synthase

merA: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

merB: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

merP: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001012.3	1.38	NRPS, PKS	meridamycin	Streptomyces violaceusniger
BGC0001275.3	0.89	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0000342.4	0.73	NRPS	enniatin	Fusarium equiseti
BGC0002135.2	0.69	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001013.5	0.69	NRPS, PKS	meridamycin	Streptomyces sp.
BGC0001135.5	0.66	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001132.4	0.64	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0000307.4	0.63	NRPS	AbT1	Aureobasidium pullulans
BGC0001128.4	0.62	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001758.4	0.62	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001012.3	0.99	NRPS, PKS	meridamycin	Streptomyces violaceusniger
BGC0001275.3	0.75	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001013.5	0.75	NRPS, PKS	meridamycin	Streptomyces sp.
BGC0000041.3	0.71	PKS	oronofacic acid	Pseudomonas syringae
BGC0001011.5	0.70	NRPS, PKS	meridamycin	Streptomyces sp. NRRL 30748
BGC0000342.4	0.69	NRPS	enniatin	Fusarium equiseti
BGC0002135.2	0.68	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.66	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0000067.5	0.66	PKS	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus
BGC0001132.4	0.65	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 91893:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Hpr) + (mal - pk - pk - ohmal) + (pk - pk - pk - Me-ccmal) + (ohmal - ohmal - pk - ohmal - ohmal - pk - ccmal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

merP: Hpr

Search NORINE for peptide: strict or relaxed

AMP-binding (502..943): Hpr

nrpys: Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: pro (Pro)
Single AA prediction:: pro (Pro)

Stachelhaus prediction details:

Stachelhaus sequence:: DIQFSAHVVK
Nearest Stachelhaus code(s):: Hpr DIQFSAHVVK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

merA: mal - pk - pk - mal

PKS_AT (578..876): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 188.1
inactive: 92.3
Methoxymalonyl-CoA: 66.7
Methylmalonyl-CoA: 50.5
Acetyl-CoA: 27.5
Propionyl-CoA: 22.9
Isobutyryl-CoA: 22.9
Ethylmalonyl-CoA: 19.3
fatty_acid: 15.7
Benzoyl-CoA: 12.6
2-Methylbutyryl-CoA: 9.5
CHC-CoA: 8.8
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (1595..1883): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 221.3
Methylmalonyl-CoA: 214.3
Methoxymalonyl-CoA: 175.0
2-Methylbutyryl-CoA: 143.4
trans-1,2-CPDA: 136.3
CHC-CoA: 125.7
Propionyl-CoA: 110.1
Malonyl-CoA: 97.5
Isobutyryl-CoA: 81.0
Benzoyl-CoA: 75.3
fatty_acid: 49.8
Acetyl-CoA: 41.3
inactive: 24.9
3-Methylbutyryl-CoA: 19.9

PKS_AT (3048..3333): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 223.7
Methylmalonyl-CoA: 219.4
Methoxymalonyl-CoA: 180.2
2-Methylbutyryl-CoA: 145.4
trans-1,2-CPDA: 145.3
CHC-CoA: 127.2
Propionyl-CoA: 120.7
Malonyl-CoA: 100.9
Isobutyryl-CoA: 82.7
Benzoyl-CoA: 71.8
fatty_acid: 48.3
Acetyl-CoA: 47.3
3-Methylbutyryl-CoA: 30.1
inactive: 25.5

PKS_AT (4722..4994): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 79.2%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 195.9
inactive: 104.2
Methoxymalonyl-CoA: 48.8
Methylmalonyl-CoA: 41.4
Propionyl-CoA: 30.8
Benzoyl-CoA: 24.2
Ethylmalonyl-CoA: 22.0
Isobutyryl-CoA: 21.2
Acetyl-CoA: 19.7
2-Methylbutyryl-CoA: 13.5
fatty_acid: 11.0
CHC-CoA: 10.5
trans-1,2-CPDA: 9.6
3-Methylbutyryl-CoA: 0.0

merB: pk - pk - pk - mmal

PKS_AT (557..855): pk

ATSignature: Methoxymalonyl-CoA

Top 3 matches:: Methoxymalonyl-CoA: 87.5%; Ethylmalonyl-CoA: 83.3%; Methylmalonyl-CoA: 79.2%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 185.2
Methylmalonyl-CoA: 178.0
Methoxymalonyl-CoA: 151.4
2-Methylbutyryl-CoA: 113.5
trans-1,2-CPDA: 112.7
Propionyl-CoA: 94.6
CHC-CoA: 87.3
Malonyl-CoA: 74.3
Isobutyryl-CoA: 60.2
fatty_acid: 36.7
Benzoyl-CoA: 35.3
Acetyl-CoA: 33.6
inactive: 20.5
3-Methylbutyryl-CoA: 9.5

PKS_AT (2293..2579): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 221.4
Methylmalonyl-CoA: 211.9
Methoxymalonyl-CoA: 173.9
2-Methylbutyryl-CoA: 154.2
trans-1,2-CPDA: 139.0
Propionyl-CoA: 116.9
CHC-CoA: 113.5
Malonyl-CoA: 88.9
Isobutyryl-CoA: 66.2
Benzoyl-CoA: 59.3
fatty_acid: 39.8
Acetyl-CoA: 37.7
3-Methylbutyryl-CoA: 31.8
inactive: 22.9

PKS_AT (3852..4140): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 212.1
Methylmalonyl-CoA: 208.7
Methoxymalonyl-CoA: 170.0
2-Methylbutyryl-CoA: 144.2
trans-1,2-CPDA: 131.7
CHC-CoA: 123.7
Propionyl-CoA: 121.1
Malonyl-CoA: 95.1
Isobutyryl-CoA: 82.5
Benzoyl-CoA: 72.6
Acetyl-CoA: 47.2
fatty_acid: 43.8
inactive: 31.0
3-Methylbutyryl-CoA: 24.9

PKS_AT (5919..6204): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 202.1
Ethylmalonyl-CoA: 201.3
Methoxymalonyl-CoA: 163.1
2-Methylbutyryl-CoA: 133.9
trans-1,2-CPDA: 133.2
CHC-CoA: 119.0
Propionyl-CoA: 104.9
Malonyl-CoA: 86.0
Isobutyryl-CoA: 71.6
Benzoyl-CoA: 67.9
fatty_acid: 40.7
Acetyl-CoA: 38.6
inactive: 19.9
3-Methylbutyryl-CoA: 8.4

merC: mal - mal - pk - mal - mal - pk - mal

PKS_AT (571..844): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 87.5%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 210.8
inactive: 115.6
Methoxymalonyl-CoA: 53.3
Methylmalonyl-CoA: 46.5
Propionyl-CoA: 37.7
Isobutyryl-CoA: 30.7
Benzoyl-CoA: 24.4
Ethylmalonyl-CoA: 23.8
Acetyl-CoA: 19.0
2-Methylbutyryl-CoA: 18.0
CHC-CoA: 14.4
fatty_acid: 14.1
trans-1,2-CPDA: 12.5
3-Methylbutyryl-CoA: 0.0

PKS_AT (2022..2295): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 87.5%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 205.0
inactive: 121.9
Methoxymalonyl-CoA: 54.6
Methylmalonyl-CoA: 48.2
Isobutyryl-CoA: 36.2
Propionyl-CoA: 35.3
Benzoyl-CoA: 25.3
Ethylmalonyl-CoA: 24.0
Acetyl-CoA: 19.1
fatty_acid: 16.9
2-Methylbutyryl-CoA: 14.3
CHC-CoA: 11.7
trans-1,2-CPDA: 9.1
3-Methylbutyryl-CoA: 0.0

PKS_AT (3472..3760): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 213.0
Methylmalonyl-CoA: 210.2
Methoxymalonyl-CoA: 169.0
2-Methylbutyryl-CoA: 143.5
trans-1,2-CPDA: 139.2
CHC-CoA: 129.5
Propionyl-CoA: 117.9
Malonyl-CoA: 94.2
Isobutyryl-CoA: 81.2
Benzoyl-CoA: 71.4
Acetyl-CoA: 53.7
fatty_acid: 37.0
3-Methylbutyryl-CoA: 26.5
inactive: 26.0

PKS_AT (5006..5279): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 87.5%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 203.7
inactive: 117.2
Methoxymalonyl-CoA: 56.3
Methylmalonyl-CoA: 47.8
Isobutyryl-CoA: 36.6
Propionyl-CoA: 34.9
Ethylmalonyl-CoA: 25.8
Benzoyl-CoA: 21.3
fatty_acid: 15.3
Acetyl-CoA: 15.2
CHC-CoA: 13.2
2-Methylbutyryl-CoA: 11.0
trans-1,2-CPDA: 8.6
3-Methylbutyryl-CoA: 0.0

PKS_AT (6465..6721): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 87.5%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 187.8
inactive: 108.5
Methoxymalonyl-CoA: 45.4
Methylmalonyl-CoA: 38.2
Acetyl-CoA: 20.8
Isobutyryl-CoA: 20.3
Propionyl-CoA: 19.2
Benzoyl-CoA: 14.8
Ethylmalonyl-CoA: 13.1
fatty_acid: 8.9
trans-1,2-CPDA: 0.0
CHC-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

PKS_AT (7994..8275): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 220.2
Methylmalonyl-CoA: 208.5
Methoxymalonyl-CoA: 166.9
2-Methylbutyryl-CoA: 148.4
trans-1,2-CPDA: 128.9
CHC-CoA: 117.8
Propionyl-CoA: 108.5
Malonyl-CoA: 84.6
Isobutyryl-CoA: 69.2
Benzoyl-CoA: 58.5
Acetyl-CoA: 46.3
fatty_acid: 35.7
3-Methylbutyryl-CoA: 17.2
inactive: 14.2

PKS_AT (10089..10384): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 75.0%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 186.7
inactive: 91.6
Methoxymalonyl-CoA: 62.6
Methylmalonyl-CoA: 54.9
Propionyl-CoA: 31.7
Isobutyryl-CoA: 29.4
Ethylmalonyl-CoA: 26.7
Acetyl-CoA: 21.2
2-Methylbutyryl-CoA: 14.2
fatty_acid: 13.1
Benzoyl-CoA: 12.9
CHC-CoA: 11.3
trans-1,2-CPDA: 9.8
3-Methylbutyryl-CoA: 0.0

Link to PARAS substrate predictor