BGC0001014: micacocidin biosynthetic gene cluster from Ralstonia solanacearum GMI1000
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Location: 1,941,939 - 1,980,062 nt. (total: 38,124 nt).
This entry is originally from NCBI GenBank AL646052.1.

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
TTA codons
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Gene details
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General
Compounds
Genes
Polyketide
NRP
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0001014
Short description micacocidin biosynthetic gene cluster from Ralstonia solanacearum GMI1000
Status Minimal annotation: no
A minimal annotation only contains information on the BGC loci and one or more linked chemical product(s)

Completeness: complete
Whether the loci encodes everything needed for the pathway producing the compound(s)
Biosynthetic class(es)
  • NRP (NRP siderophore)
  • Polyketide (Other)
Loci NCBI GenBank: AL646052.1
Compounds
  • micacocidin
Species Ralstonia solanacearum GMI1000 [taxonomy]
References
Chemical products information
micacocidin
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C27H39N3O4S3
Chemical database entries
NPAtlas
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • RSc1804
  • CAD15506.1
 1941939 - 1944437 (-) protein with thioesterase and phosphopantetheinyl transferase domains
  • Activation / processing
Activity assay
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  • RSc1805
  • CAD15507.1
 1944434 - 1945597 (-) thiazolinyl imide reductase
  • Tailoring (Reduction)
Activity assay
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  • RSc1806
  • CAD15508.1
 1945814 - 1958620 (-) polyketide synthase/non-ribosomal peptide synthetase
  • Scaffold biosynthesis
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  • RSc1807
  • CAD15509.1
 1958894 - 1960969 (+) TonB-dependent receptor
  • Transport
Sequence-based prediction
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  • RSc1808
  • CAD15510.1
 1961077 - 1962933 (+) ATP-binding transmembrane ABC transporter protein
  • Transport
Sequence-based prediction
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  • RSc1809
  • CAD15511.1
 1962930 - 1964675 (+) ATP-binding transmembrane ABC transporter protein
  • Transport
Sequence-based prediction
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  • RSc1810
  • CAD15512.1
 1964976 - 1972118 (-) polyketide synthase
  • Scaffold biosynthesis
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  • RSc1811
  • CAD15513.1
 1972550 - 1978570 (+) non-ribosomal peptide synthetase
  • Scaffold biosynthesis
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  • RSc1812
  • CAD15514.1
 1978660 - 1979076 (+) conserved hypothetical protein
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  • RSc1813
  • CAD15515.1
 1979151 - 1980062 (-) AraC-like regulator
  • Regulation
Sequence-based prediction
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Polyketide-specific information
Subclass Other
Starter unit Hexanoyl-ACP
Cyclic? yes
Polyketide-synthases
Genes Properties Modules
RSc1806
+
RSc1810
+
RSc1806 		Synthase subclass: Modular type I/Iterative type I
Iterative PKS type: Partially reducing, C2-C7 first
Iteration counts: 2
Module 1
Specificity: Malonyl-CoA
Evidence for specificity: Activity assay
Genes: RSc1806
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: Unknown
Non-canonical activity:
  • Iterated
Evidence for non-canonical activity:
  • Activity assay
Module 4
Genes: RSc1810
Core domains: Ketosynthase, Ketoreductase, Thiolation (ACP/PCP)
Scaffold-modifying domains: Methylation
KR-domain stereochemistry: Unknown
NRP-specific information
Subclass NRP siderophore
Cyclic? no
Release type
  • Hydrolysis
NRP-synthases
Gene Modules
RSc1806
Module 0
Module 2
Specificity: cysteine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: Heterocyclization
Modification domains: Methylation
Module 3
Condensation domain type: Heterocyclization
RSc1811
Module 5
Specificity: cysteine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: Heterocyclization
Modification domains: Methylation
Annotation changelog
MIBiG version Submitter Notes
1.0
  • Hidden contributor (ID: YETYK72PRB3MT456E3R3XHBM, no GDPR consent given).
  • Submitted
2.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Migrated from v1.4
3.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Linked new NPAtlas id for micacocidin (npatlas:NPA01634)
  • Corrected NRP module activity
  • Changed amino acid substrates to lower case
3.1
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Update chemical activity to schema version 2.11
Detailed domain annotation
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