Genomic loci for this biosynthetic pathway

Cluster Type From To
The following clusters are from record BGC0001014.1:
Cluster 1NRP / Polyketide138124

BGC0001014, micacocidin biosynthetic gene cluster from Ralstonia solanacearum. Locus 1. Full MIBiG entry.

Chemical compounds

Compound: micacocidin
SMILES string: Copy to clipboard
Molecular formula: C27H39N3O4S3
Exact molecular mass: 566.2175 Da ([M+H]+)
Molecular activity: Antibacterial, Siderophore

Class-specific details

Biosynthetic class(es):
NRP / Polyketide
NRP siderophore (linear)
Thioesterase type:
Type I & II
Release / cyclization type:
Hydrolysis

Nonribosomal peptide synthetases:
micC (CAD15508.1)
Module 0
A specificity: None
Evidence for specificity: Activity assay
C domain subtype: N/A
Module 2
A specificity: Cysteine
Evidence for specificity: Structure-based inference
C domain subtype: Heterocyclization
Scaffold-modifying domain: Methylation
Module 3
A specificity: Unknown
Evidence for specificity: Unknown
C domain subtype: Heterocyclization
micH (CAD15513.1)
Module 5
A specificity: Cysteine
Evidence for specificity: Structure-based inference
C domain subtype: Heterocyclization
Scaffold-modifying domain: Methylation
Polyketide subclass:
Other (cyclic)
Polyketide synthase subclass:
Modular type I / Iterative type I
Starter unit:
Hexanoyl-ACP
Polyketide synthase / ketosynthase-encoding genes:
RSC1806
Iterative PKS subtype:
Partially reducing
Number of iterations:
2
C2-C7 first
Thioesterase type:
None
Release / cyclization type:
Other

Modular polyketide synthases:
micC (CAD15508.1)
Module 1
AT specificity: Malonyl-CoA
Evidence for specificity: Activity assay
KR stereochemistry: Unknown
Scaffold-modifying domain: None
Module is iterated
Evidence for iteration: Unknown

micG (CAD15512.1)
Module 4
KR stereochemistry: Unknown
Scaffold-modifying domain: Methylation

Gene cluster description

micacocidin (BGC0001014). Gene Cluster 1. Biosynthetic class = NRP/Polyketide. GenBank AL646052, positions 1941939-1980062. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Domain annotation

General MIBiG information on this cluster

Complete gene cluster sequence?complete
Evidence for cluster-compound connection:Knock-out studies, Enzymatic assays, Heterologous expression
MIxS-compliance:Unknown
Contact for this cluster:Markus Nett (Hans-Knoell-Institute)

Literature references

1. Kreutzer MF et al. (2011) Biosynthesis of a complex yersiniabactin-like natural product via the mic locus in phytopathogen Ralstonia solanacearum. Appl Environ Microbiol 77(17):6117-24. doi: 10.1128/AEM.05198-11. Epub
2. Kage H et al. (2013) An iterative type I polyketide synthase initiates the biosynthesis of the antimycoplasma agent micacocidin. Chem Biol 20(6):764-71. doi: 10.1016/j.chembiol.2013.04.010.
3. Kreutzer MF et al. (2014) Precursor-directed biosynthesis of micacocidin derivatives with activity against Mycoplasma pneumoniae. Org Biomol Chem 12(1):113-8. doi: 10.1039/c3ob41839a. Epub 2013 Nov