BGC0001015 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001015 (Planktothrix agardhii NIVA-CYA 126/8)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	NRPS,T1PKS	1	55,575	High	microcystin	NRPS:Type I+PKS

BGC0001015 - Region 1 - NRPS,T1PKS

Download region GenBank file

Download region SVG

Location: 1 - 55,575 nt. (total: 55,575 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 55,576
Label		BGC0001015

Tailoring functions

Transferases: methyltransferase

mcyJ: methyltransferase

smcogs: SMCOG1089: methyltransferase

Hydrolases: esterase

mcyT: thioesterase

smcogs: SMCOG1004: thioesterase

Ligases: ligase synthase

mcyA: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

mcyB: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

mcyC: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

mcyD: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

mcyE: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

mcyG: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001015.5	1.74	NRPS, PKS	microcystin	Planktothrix agardhii NIVA-CYA 126/8
BGC0001276.3	0.89	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0002135.2	0.88	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001833.4	0.84	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001758.4	0.83	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001135.5	0.82	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001128.4	0.81	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.79	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002071.2	0.79	NRPS	virginiafactin A, virginiafactin B, virginiafactin C, virginiafactin D	Pseudomonas sp. QS1027
BGC0001132.4	0.79	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001015.5	0.98	NRPS, PKS	microcystin	Planktothrix agardhii NIVA-CYA 126/8
BGC0001276.3	0.75	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0002135.2	0.75	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001833.4	0.75	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001758.4	0.74	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001128.4	0.74	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002075.3	0.73	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0001135.5	0.73	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001132.4	0.73	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002286.3	0.73	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Similar known clusters from MIBiG 4.0

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 55575:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-ccmal - ccmal) + (mal - X - X) + (Me-ohmal) + (NMe-Ser - D-Ala) + (Arg - X) + (Arg)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

mcyD: mal - mal

PKS_AT (580..790): mal

ATSignature: (unknown)

No matches above 50%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 27.6
inactive: 27.4
2-Methylbutyryl-CoA: 10.8
Methylmalonyl-CoA: 9.6
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Propionyl-CoA: 0.0
Methoxymalonyl-CoA: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (2641..2925): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 87.5%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 190.0
inactive: 140.0
Methoxymalonyl-CoA: 66.8
Methylmalonyl-CoA: 63.8
Propionyl-CoA: 39.5
trans-1,2-CPDA: 25.0
Isobutyryl-CoA: 22.6
Ethylmalonyl-CoA: 21.0
Benzoyl-CoA: 20.9
2-Methylbutyryl-CoA: 19.0
Acetyl-CoA: 14.9
CHC-CoA: 13.5
3-Methylbutyryl-CoA: 13.4
fatty_acid: 11.5

mcyE: mal - X

PKS_AT (561..851): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 79.2%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 151.4
inactive: 108.8
Methoxymalonyl-CoA: 46.9
Methylmalonyl-CoA: 39.1
Propionyl-CoA: 28.2
Isobutyryl-CoA: 28.0
3-Methylbutyryl-CoA: 22.1
Benzoyl-CoA: 20.4
Acetyl-CoA: 12.3
CHC-CoA: 8.5
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Ethylmalonyl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

AMP-binding (2447..2842): X

nrpys: D-Glu

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: glu,gln (Glu, Gln)
Single AA prediction:: glu (Glu)

Stachelhaus prediction details:

Stachelhaus sequence:: DPRHSGVVGK
Nearest Stachelhaus code(s):: D-Glu DPRHSGVVGK (100% 8Å match); Glu or D-Glu DPRHSGVVGK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

mcyG: X - mal

AMP-binding (45..454): X

nrpys: Cin

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: AILWVAASGK
Nearest Stachelhaus code(s):: Cin AILWVAASGK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

PKS_AT (1225..1519): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 91.7%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 176.4
inactive: 122.2
Methoxymalonyl-CoA: 65.3
Methylmalonyl-CoA: 65.1
Propionyl-CoA: 39.4
Ethylmalonyl-CoA: 31.2
Acetyl-CoA: 29.4
2-Methylbutyryl-CoA: 27.5
Isobutyryl-CoA: 25.0
Benzoyl-CoA: 23.2
CHC-CoA: 20.8
fatty_acid: 19.5
trans-1,2-CPDA: 16.4
3-Methylbutyryl-CoA: 10.6

mcyA: Ser - Ala

Search NORINE for peptide: strict or relaxed

AMP-binding (260..668): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: DVWHISLIDK
Nearest Stachelhaus code(s):: Ser DVWHISLIDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1749..2150): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: ala (Ala)

Stachelhaus prediction details:

Stachelhaus sequence:: DLFNNALSYK
Nearest Stachelhaus code(s):: Ala DLFNNALSYK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

mcyB: Arg - X

Search NORINE for peptide: strict or relaxed

AMP-binding (479..879): Arg

nrpys: Arg

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: leu (Leu)

Stachelhaus prediction details:

Stachelhaus sequence:: DALFFGLVDK
Nearest Stachelhaus code(s):: Arg DALFFGLVDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1534..1965): X

nrpys: D-Asp

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DPRHVGIFIK
Nearest Stachelhaus code(s):: D-Asp DPRHVGIFIK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

mcyC: Arg

Search NORINE for peptide: strict or relaxed

AMP-binding (483..880): Arg

nrpys: Arg

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: glu,gln (Glu, Gln)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DPWGFGLVDK
Nearest Stachelhaus code(s):: Arg DPWGFGLVDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

Link to PARAS substrate predictor