BGC0001016 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001016 (Anabaena sp. 90)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS,NRPS,NRPS-like	1	57,817	microcystin	NRPS:Type I+PKS	100%

BGC0001016 - Region 1 - NRPS,NRPS-like,T1PKS

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Location: 1 - 57,817 nt. (total: 57,817 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: cds(Condensation and (AMP-binding or A-OX))
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 57,818
Label		BGC0001016

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001016	2.76	NRP, Polyketide	microcystin	Anabaena sp. 90
BGC0001133	1.43	NRP	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0001705	1.42	NRP, Polyketide	nodularin	Nostoc sp. CENA543
BGC0002071	1.33	NRP	virginiafactin A, virginiafactin B, virginiafactin C, virginiafactin D	Pseudomonas sp. QS1027
BGC0002135	1.30	NRP	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0002437	1.29	NRP	thermoactinoamide A	Thermoactinomyces sp. AS95
BGC0001667	1.24	NRP, Polyketide	microcystin-LR, microcystin-FR, microcystin-LA, microcystin-LAba, microcystin-LM, microcystin-LV, microcystin-LL	Fischerella sp. CENA161
BGC0001629	1.22	NRP	jessenipeptin	Pseudomonas sp. QS1027
BGC0000465	1.21	NRP	xenortide A, xenortide B, xenortide C, xenortide D	Xenorhabdus nematophila ATCC 19061
BGC0000426	1.18	NRP	sevadicin	Paenibacillus larvae

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001016	1.00	NRP, Polyketide	microcystin	Anabaena sp. 90
BGC0001705	0.84	NRP, Polyketide	nodularin	Nostoc sp. CENA543
BGC0001133	0.74	NRP	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0000465	0.72	NRP	xenortide A, xenortide B, xenortide C, xenortide D	Xenorhabdus nematophila ATCC 19061
BGC0002071	0.71	NRP	virginiafactin A, virginiafactin B, virginiafactin C, virginiafactin D	Pseudomonas sp. QS1027
BGC0000426	0.71	NRP	sevadicin	Paenibacillus larvae
BGC0001667	0.70	NRP, Polyketide	microcystin-LR, microcystin-FR, microcystin-LA, microcystin-LAba, microcystin-LM, microcystin-LV, microcystin-LL	Fischerella sp. CENA161
BGC0001629	0.70	NRP	jessenipeptin	Pseudomonas sp. QS1027
BGC0002135	0.68	NRP	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0002437	0.68	NRP	thermoactinoamide A	Thermoactinomyces sp. AS95

Similar known gene clusters from MIBiG 4.0

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 57817:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal - X) + (ccmal) + (Me-ohmal) + (NMe-Ser - D-Ala) + (X - X) + (Arg)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 0 - 56324:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal - X) + (ccmal) + (Me-ohmal) + (NMe-Ser - D-Ala) + (X - X) + (Arg)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 17521 - 57817:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (ccmal) + (Me-ohmal) + (NMe-Ser - D-Ala) + (X - X) + (Arg)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

mcyE

PKS_AT (561..850): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 75.0%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 163.7
inactive: 118.1
Methoxymalonyl-CoA: 53.2
Methylmalonyl-CoA: 48.1
Isobutyryl-CoA: 42.9
Propionyl-CoA: 42.0
3-Methylbutyryl-CoA: 22.1
Benzoyl-CoA: 17.6
Acetyl-CoA: 16.2
CHC-CoA: 11.7
fatty_acid: 11.1
Ethylmalonyl-CoA: 8.7
trans-1,2-CPDA: 0.0
2-Methylbutyryl-CoA: 0.0

AMP-binding (2443..2838): X

nrpys: Glu, D-Glu

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: glu,gln (Glu, Gln)
Single AA prediction:: glu (Glu)

Stachelhaus prediction details:

Stachelhaus sequence:: DPRHSGVVGK
Nearest Stachelhaus code(s):: Glu or D-Glu DPRHSGVVGK (100% 8Å match); D-Glu DPRHSGVVGK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

mcyD

PKS_AT (2613..2897): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 87.5%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 203.7
inactive: 145.2
Methylmalonyl-CoA: 73.1
Methoxymalonyl-CoA: 72.9
Propionyl-CoA: 45.2
Isobutyryl-CoA: 37.4
Ethylmalonyl-CoA: 31.7
Benzoyl-CoA: 26.3
2-Methylbutyryl-CoA: 25.6
Acetyl-CoA: 22.3
trans-1,2-CPDA: 21.9
CHC-CoA: 17.8
fatty_acid: 13.6
3-Methylbutyryl-CoA: 8.3

mcyG

CAL_domain (67..490): pk

minowa: fatty_acid

Prediction, score:

fatty_acid: 21.8
shikimic_acid: 0.0
NH2: 0.0
Acetyl-CoA: 0.0
AHBA: 0.0

PKS_AT (1241..1534): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 87.5%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 181.6
inactive: 128.1
Methylmalonyl-CoA: 74.7
Methoxymalonyl-CoA: 74.4
Propionyl-CoA: 40.2
Ethylmalonyl-CoA: 37.3
Isobutyryl-CoA: 36.4
2-Methylbutyryl-CoA: 33.5
Acetyl-CoA: 32.2
Benzoyl-CoA: 29.0
CHC-CoA: 27.9
trans-1,2-CPDA: 23.2
fatty_acid: 17.3
3-Methylbutyryl-CoA: 14.9

mcyA

Search NORINE for peptide: strict or relaxed

AMP-binding (255..652): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: DVWHISLIDK
Nearest Stachelhaus code(s):: Ser DVWHISLIDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1740..2130): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: ala (Ala)

Stachelhaus prediction details:

Stachelhaus sequence:: DLFNNALTYK
Nearest Stachelhaus code(s):: Ala DLFNNALTYK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

mcyB

AMP-binding (479..876): X

nrpys: Leu, Arg

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: leu (Leu)

Stachelhaus prediction details:

Stachelhaus sequence:: DVWFFGLVDK
Nearest Stachelhaus code(s):: Leu or Arg DVWFFGLVDK (100% 8Å match); Ser DVWFFGLVDK (85% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1529..1954): X

nrpys: D-bMe-Asp

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DARHVGIFVK
Nearest Stachelhaus code(s):: D-bMe-Asp DARHVGIFVK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

mcyC

Search NORINE for peptide: strict or relaxed

AMP-binding (475..872): Arg

nrpys: Arg

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVWCFGLVDK
Nearest Stachelhaus code(s):: Arg DVWCFGLVDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)