BGC0001016: microcystin biosynthetic gene cluster from Anabaena sp. 90
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Location: 1 - 57,817 nt. (total: 57,817 nt).
This entry is originally from NCBI GenBank AY212249.1.

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
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Gene details
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General
Compounds
Genes
Biosynthesis
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0001016
Short description microcystin biosynthetic gene cluster from Anabaena sp. 90
Status Quality: questionable
The quality level of this entry.

Status: active
The status of this entry.

Completeness: complete
Whether the entry covers everything needed for the pathway producing the compound(s)
Biosynthetic class(es)
  • NRPS (Type I)
  • PKS (Unknown)
Loci
AY212249.1
via
Compounds
  • microcystin
Species Anabaena sp. 90 [taxonomy]
References
Chemical products information
microcystin Evidence:
Copy SMILES
C49H74N10O12
Chemical database entries
PubCHEM
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • AAO62577.1
 174 - 566 (+) hypothetical protein
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    • AAO62578.1
    		 972 - 1328 (+) hypothetical protein
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      • AAO62579.1
      • mcyH
      		 1301 - 3079 (-) ABC transporter ATP-binding-like protein
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        • AAO62580.1
        • mcyI
        		 3374 - 4387 (-) phosphoglycerate dehydrogenase-like protein
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          • AAO62581.1
          • mcyF
          		 4438 - 5196 (-) amino acid racemase
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            • AAO62582.1
            • mcyE
            		 5238 - 15686 (-) polyketide synthase peptide sythetase fusion protein
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              • AAO62583.1
              • mcyJ
              		 15723 - 16655 (-) putative O-methyl transferase
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                • AAO62584.1
                • mcyD
                		 16714 - 28323 (-) polyketide synthase type 1
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                  • AAO62585.1
                  • mcyG
                  		 28417 - 36324 (-) peptide sythetase polyketide synthase fusion protein
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                    • AAO62586.1
                    • mcyA
                    		 37522 - 45888 (+) peptide sythetase
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                      • AAO62587.1
                      • mcyB
                      		 45906 - 52307 (+) peptide sythetase
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                        • AAO62588.1
                        • mcyC
                        		 52304 - 56158 (+) peptide sythetase
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                          • AAO62589.1
                          • atn1
                          		 56675 - 57817 (+) putative transposase
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                            Biosynthesis information

                            Biosynthetic modules

                            Name
                            11
                            Type
                            nrps-type1
                            Genes
                            mcyC
                            Substrates
                            arginine (evidence: Structure-based inference [1], [2])
                            Integrated Monomers
                            Domains
                            condensation (LCL), adenylation
                            Name
                            9
                            Type
                            nrps-type1
                            Genes
                            mcyB
                            Substrates
                            leucine, arginine (evidence: Structure-based inference [1], [2])
                            Integrated Monomers
                            Domains
                            condensation (DCL), adenylation
                            Name
                            10
                            Type
                            nrps-type1
                            Genes
                            mcyB
                            Substrates
                            3S-methyl-D-aspartic acid branched, aspartic acid branched (evidence: Structure-based inference [1], [2])
                            Integrated Monomers
                            Domains
                            condensation (LCL), adenylation
                            Name
                            7
                            Type
                            nrps-type1
                            Genes
                            mcyA
                            Substrates
                            serine (evidence: Structure-based inference, Homology)
                            Integrated Monomers
                            Domains
                            adenylation, methyltransferase
                            Name
                            8
                            Type
                            nrps-type1
                            Genes
                            mcyA
                            Substrates
                            alanine (evidence: Structure-based inference [1], [2])
                            Integrated Monomers
                            Domains
                            condensation (DCL), adenylation, epimerase
                            Name
                            0
                            Type
                            nrps-type1
                            Genes
                            mcyG
                            Substrates
                            cinnamic acid, hydrocinnamic acid, D-phenylalanine (evidence: Structure-based inference, Homology)
                            Integrated Monomers
                            Domains
                            adenylation
                            Name
                            6
                            Type
                            nrps-type1
                            Genes
                            mcyE
                            Substrates
                            D-glutamic acid branched (evidence: Structure-based inference [1], [2])
                            Integrated Monomers
                            Domains
                            condensation (LCL), adenylation
                            Name
                            1
                            Type
                            pks-modular
                            Genes
                            mcyG
                            Substrates
                            Integrated Monomers
                            Domains
                            acyltransferase, ketosynthase, acyltransferase, ketoreductase, methyltransferase, carrier (ACP)
                            Name
                            2
                            Type
                            pks-trans-at
                            Genes
                            mcyD
                            Substrates
                            Integrated Monomers
                            Domains
                            ketosynthase, dehydratase, ketoreductase, methyltransferase, carrier (ACP)
                            Name
                            3
                            Type
                            pks-modular
                            Genes
                            mcyD
                            Substrates
                            Integrated Monomers
                            Domains
                            acyltransferase, ketosynthase, acyltransferase, dehydratase, ketoreductase, carrier (ACP)
                            Name
                            4
                            Type
                            pks-modular
                            Genes
                            mcyE
                            Substrates
                            Integrated Monomers
                            Domains
                            acyltransferase, ketosynthase, acyltransferase, methyltransferase, carrier (ACP)
                            Annotation changelog

                            Entry version: 4

                            Date
                            Changes
                            Submitters
                            Reviewers
                            2022-10-07
                            Update chemical activity to schema version 2.11
                            • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                            • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                            Entry version: 3

                            Date
                            Changes
                            Submitters
                            Reviewers
                            2022-09-15
                            Corrected NRP module activity
                            • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                            • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                            2022-09-15
                            Removed ketoreductase stereochemistry annotation from modules without ketoreductases
                            • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                            • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                            2022-09-15
                            Updated NRP substrate specificities
                            • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                            • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                            Entry version: 2

                            Date
                            Changes
                            Submitters
                            Reviewers
                            2019-10-16
                            Migrated from v1.4
                            • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                            • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                            Entry version: 1

                            Date
                            Changes
                            Submitters
                            Reviewers
                            2015-06-12
                            Submitted
                            • (ID: BAJIZJS2YANDM4M7JHS5DFUK)
                            • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                            Detailed domain annotation
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