Genomic loci for this biosynthetic pathway

Cluster Type From To
The following clusters are from record BGC0001016.1:
Cluster 1NRP / Polyketide157817

BGC0001016, microcystin biosynthetic gene cluster from Anabaena sp. 90. Locus 1. Full MIBiG entry.

Chemical compounds

Compound: microcystin
PubChem ID: 445434
SMILES string: Copy to clipboard
Molecular formula: C49H74N10O12
Average molecular mass: 995.189 Da
Molecular activity: Other
Molecular target: Eukaryotic protein phosphatase 1 and 2A

Class-specific details

Biosynthetic class(es):
NRP / Polyketide
Other (cyclic)
Thioesterase type:
None
Release / cyclization type:
Hydrolysis

Nonribosomal peptide synthetases:
mcyC (AAO62588.1)
Module 11
A specificity: Arginine
Evidence for specificity: None
C domain subtype: LCL
mcyB (AAO62587.1)
Module 9
A specificity: Leucine / Arginine
Evidence for specificity: None
C domain subtype: DCL
Module 10
A specificity: Aspartate / N-methylaspartate
Evidence for specificity: None
C domain subtype: LCL
mcyA (AAO62586.1)
Module 7
A specificity: Serine
Evidence for specificity: None
C domain subtype: N/A
Scaffold-modifying domain: Methylation
Module 8
A specificity: Alanine
Evidence for specificity: None
AA is epimerized
C domain subtype: DCL
mcyG (AAO62585.1)
Module 0
A specificity: Hydrocinnamate
Evidence for specificity: None
C domain subtype: N/A
mcyE (AAO62582.1)
Module 5
A specificity: Unknown
Evidence for specificity: Unknown
C domain subtype: LCL
Scaffold-modifying domain: Aminotransferase
Module 6
A specificity: Glutamine
Evidence for specificity: None
C domain subtype: LCL

Modular polyketide synthases:
mcyG (AAO62585.1)
Module 1
AT specificity: Malonyl-CoA
Evidence for specificity: None
KR stereochemistry: Unknown
Scaffold-modifying domain: Methylation

mcyD (AAO62584.1)
Module 2
KR stereochemistry: Unknown
Scaffold-modifying domain: Methylation
Module 3
AT specificity: Malonyl-CoA
Evidence for specificity: None
KR stereochemistry: Unknown
Scaffold-modifying domain: None

mcyE (AAO62582.1)
Module 4
AT specificity: Malonyl-CoA
Evidence for specificity: None
Scaffold-modifying domain: Methylation

Gene cluster description

microcystin (BGC0001016). Gene Cluster 1. Biosynthetic class = NRP/Polyketide. GenBank AY212249. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Domain annotation

Homologous known gene clusters

General MIBiG information on this cluster

Complete gene cluster sequence?complete
Evidence for cluster-compound connection:Sequence-based prediction
MIxS-compliance:Unknown
Contact for this cluster:David Fewer, annotation team (University of Helsinki)

Literature references

1. Rouhiainen L et al. (2004) Genes coding for hepatotoxic heptapeptides (microcystins) in the cyanobacterium Anabaena strain 90. Appl Environ Microbiol 70(2):686-92.
2. Hicks LM et al. (2006) Structural characterization of in vitro and in vivo intermediates on the loading module of microcystin synthetase. ACS Chem Biol 1(2):93-102. doi: 10.1021/cb500007v.