BGC0001019: microsclerodermin M biosynthetic gene cluster from Sorangium cellulosum
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Location: 1 - 58,049 nt. (total: 58,049 nt).
This entry is originally from NCBI GenBank KF657738.1.

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
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resistance
TTA codons
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Gene details
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General
Compounds
Genes
Polyketide
NRP
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0001019
Short description microsclerodermin M biosynthetic gene cluster from Sorangium cellulosum
Status Minimal annotation: no
A minimal annotation only contains information on the BGC loci and one or more linked chemical product(s)

Completeness: complete
Whether the loci encodes everything needed for the pathway producing the compound(s)
Remarks "module 1 has 3 iterations, module 3 has 2 iterations as inferred from molecule structure."
Biosynthetic class(es)
  • NRP
  • Polyketide (Aryl polyene)
Loci NCBI GenBank: KF657738.1
Compounds
  • microsclerodermin M
Species Sorangium cellulosum [taxonomy]
References
Chemical products information
microsclerodermin M
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C44H54N8O12
Chemical database entries
NPAtlas
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • AHB82049.1
  • mscK
 1 - 1248 (-) MFS transporter
  • Transport
Sequence-based prediction
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  • AHB82050.1
  • mscJ
 1473 - 2246 (+) Thioesterase II
  • Other enzymatic
Sequence-based prediction
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  • AHB82051.1
  • mscA
 3859 - 13686 (+) polyketide synthase
  • Scaffold biosynthesis
Sequence-based prediction
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  • AHB82052.1
  • mscB
 13709 - 16321 (+) polyketide synthase
  • Scaffold biosynthesis
Sequence-based prediction
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  • AHB82053.1
  • mscC
 16327 - 20982 (+) polyketide synthase
  • Scaffold biosynthesis
Sequence-based prediction
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  • AHB82054.1
  • mscD
 20995 - 23697 (+) polyketide synthase
  • Scaffold biosynthesis
Sequence-based prediction
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  • AHB82055.1
  • mscE
 23727 - 25067 (+) Putative Amidohydrolase
  • Unknown
Sequence-based prediction
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  • AHB82056.1
  • mscF
 25335 - 32156 (+) non ribosomal peptide synthetase
  • Scaffold biosynthesis
Sequence-based prediction
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  • AHB82057.1
  • mscG
 32158 - 36804 (+) polyketide synthase
  • Scaffold biosynthesis
Sequence-based prediction
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  • AHB82058.1
  • mscH
 36844 - 49269 (+) non ribosomal peptide synthetase
  • Scaffold biosynthesis
Sequence-based prediction
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  • AHB82059.1
  • mscI
 49344 - 58049 (+) non ribosomal peptide synthetase/polyketide synthase
  • Scaffold biosynthesis
Sequence-based prediction
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Polyketide-specific information
Subclass Aryl polyene
Cyclic? yes
Release type
  • Macrolactamization
Polyketide-synthases
Genes Properties Modules
mscG
+
mscF
+
mscD
+
mscC
+
mscB
+
mscI
+
mscA 		Synthase subclass: Modular type I
Thioesterases:
  • mscI (Type I)
Module 1
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: mscA
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Dehydratase, CoA-ligase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
Non-canonical activity:
  • Iterated
Evidence for non-canonical activity:
  • Structure-based inference
Module 2
Genes: mscB
Core domains: Ketosynthase, Acyltransferase
Non-canonical activity:
  • Skipped
Evidence for non-canonical activity:
  • Structure-based inference
Module 3
Specificity: Hydroxymalonyl-CoA
Evidence for specificity: Structure-based inference
Genes: mscC
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: D-OH
Non-canonical activity:
  • Iterated
Evidence for non-canonical activity:
  • Structure-based inference
Module 4
Genes: mscD
Core domains: Ketosynthase, Acyltransferase
Non-canonical activity:
  • Skipped
Evidence for non-canonical activity:
  • Structure-based inference
Module 5
Genes: mscF
Core domains: Thiolation (ACP/PCP)
Module 6
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: mscG
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
Module 11
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: mscI
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
NRP-specific information
Subclass N/A
Cyclic? yes
Release type
  • Macrolactamization
NRP-synthases
Gene Modules
mscA
Module 0
mscF
Module 5
Specificity: asparagine
Evidence for specificity:
  • Feeding study
  • Structure-based inference
  • Sequence-based prediction
Condensation domain type: LCL
mscH
Module 7
Specificity: glycine
Evidence for specificity:
  • Structure-based inference
  • Sequence-based prediction
Condensation domain type: LCL
Modification domains: Methylation
Module 8
Specificity: tryptophan
Evidence for specificity:
  • Structure-based inference
  • Sequence-based prediction
Condensation domain type: LCL
Module 9
Specificity: glycine
Evidence for specificity:
  • Structure-based inference
  • Sequence-based prediction
Condensation domain type: DCL
mscI
Module 10
Specificity: glycine
Evidence for specificity:
  • Structure-based inference
  • Sequence-based prediction
Condensation domain type: LCL
Annotation changelog
MIBiG version Submitter Notes
1.0
  • Hidden contributor (ID: RZRTXUKFH6LMF4VTOZ22YQ5Y, no GDPR consent given).
  • Submitted
2.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Migrated from v1.4
  • Updated compound(s) information (NPAtlas curation)
3.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Corrected NRP module activity
  • Removed ketoreductase stereochemistry annotation from modules without ketoreductases
  • Corrected NRP gene names
  • Updated NRP substrate specificities
3.1
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Update chemical activity to schema version 2.11
Detailed domain annotation
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