BGC0001022 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001022 (Stigmatella aurantiaca)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS,NRPS	1	49,736	High	myxalamid/myxalamid A/myxalamid C/myxalamid D	NRPS:Type I+PKS

BGC0001022 - Region 1 - NRPS,T1PKS

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Location: 1 - 49,736 nt. (total: 49,736 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 49,737
Label		BGC0001022

Tailoring functions

Oxidoreductases: dehydrogenase

mxaC: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Transferases: acylase

mxaE: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

Hydrolases: hydrolase

AAK57191.1: alpha/beta hydrolase fold protein

smcogs: SMCOG1036: alpha/beta hydrolase fold protein

Ligases: ligase synthase

mxaA: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

mxaB2: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

mxaD: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

mxaF: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001022.5	1.38	NRPS, PKS	myxalamid, myxalamid A, myxalamid C, myxalamid D	Stigmatella aurantiaca
BGC0001273.3	0.83	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.82	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244.3	0.82	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001275.3	0.81	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001268.4	0.68	NRPS, PKS	fusarin C	Fusarium fujikuroi
BGC0002192.3	0.67	PKS	FR901512	fungal sp. No.14919
BGC0002191.2	0.67	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001284.4	0.66	PKS	alternariol	Parastagonospora nodorum SN15
BGC0001164.3	0.64	PKS	1-heptadecene	Cyanothece sp. PCC 7822

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001022.5	1.00	NRPS, PKS	myxalamid, myxalamid A, myxalamid C, myxalamid D	Stigmatella aurantiaca
BGC0001273.3	0.77	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.77	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244.3	0.77	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001275.3	0.77	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0000018.5	0.68	PKS	angolamycin	Streptomyces eurythermus
BGC0001268.4	0.67	NRPS, PKS	fusarin C	Fusarium fujikuroi
BGC0002192.3	0.67	PKS	FR901512	fungal sp. No.14919
BGC0001284.4	0.66	PKS	alternariol	Parastagonospora nodorum SN15
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 49736:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-ccmal) + (Me-ccmal) + (pk - ohmal - ccmal) + (Me-redmal) + (Ala)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 0 - 27822:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk - ohmal - ccmal) + (Me-redmal) + (Ala)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

mxaA: Ala

Search NORINE for peptide: strict or relaxed

AMP-binding (542..932): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: ala (Ala)

Stachelhaus prediction details:

Stachelhaus sequence:: DLFNNALTYK
Nearest Stachelhaus code(s):: Ala DLFNNALTYK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

mxaB2: mmal

PKS_AT (570..868): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Ethylmalonyl-CoA: 79.2%; Malonyl-CoA: 79.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 180.0
Ethylmalonyl-CoA: 155.3
Methoxymalonyl-CoA: 137.6
Malonyl-CoA: 112.7
Isobutyryl-CoA: 97.9
Benzoyl-CoA: 95.7
Acetyl-CoA: 89.4
2-Methylbutyryl-CoA: 88.2
CHC-CoA: 79.4
trans-1,2-CPDA: 78.8
Propionyl-CoA: 76.7
3-Methylbutyryl-CoA: 51.1
fatty_acid: 42.1
inactive: 26.1

mxaC: pk - mal - mal

PKS_AT (560..858): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; Methylmalonyl-CoA: 87.5%; Ethylmalonyl-CoA: 70.8%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 142.3
Ethylmalonyl-CoA: 134.9
Malonyl-CoA: 125.2
Methoxymalonyl-CoA: 114.4
2-Methylbutyryl-CoA: 87.3
Isobutyryl-CoA: 84.5
Acetyl-CoA: 78.6
Propionyl-CoA: 65.2
Benzoyl-CoA: 63.9
trans-1,2-CPDA: 63.1
inactive: 40.7
CHC-CoA: 35.9
3-Methylbutyryl-CoA: 34.6
fatty_acid: 29.4

PKS_AT (2373..2669): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; Methylmalonyl-CoA: 75.0%; Propionyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 138.3
Methylmalonyl-CoA: 136.2
Methoxymalonyl-CoA: 126.8
Isobutyryl-CoA: 119.6
Ethylmalonyl-CoA: 101.7
trans-1,2-CPDA: 70.2
Propionyl-CoA: 70.0
2-Methylbutyryl-CoA: 65.8
Acetyl-CoA: 65.2
Benzoyl-CoA: 64.9
3-Methylbutyryl-CoA: 58.1
inactive: 54.1
CHC-CoA: 52.3
fatty_acid: 40.1

PKS_AT (3904..4197): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 91.7%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 207.9
inactive: 163.9
Methoxymalonyl-CoA: 88.9
Methylmalonyl-CoA: 82.9
Propionyl-CoA: 70.2
Isobutyryl-CoA: 50.0
Ethylmalonyl-CoA: 47.7
2-Methylbutyryl-CoA: 45.0
CHC-CoA: 41.4
Benzoyl-CoA: 32.9
trans-1,2-CPDA: 29.4
Acetyl-CoA: 20.7
3-Methylbutyryl-CoA: 20.0
fatty_acid: 17.2

mxaD: mmal

PKS_AT (551..848): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Malonyl-CoA: 87.5%; Ethylmalonyl-CoA: 79.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 175.0
Ethylmalonyl-CoA: 151.3
Methoxymalonyl-CoA: 127.0
Benzoyl-CoA: 103.3
Malonyl-CoA: 92.2
trans-1,2-CPDA: 90.5
Isobutyryl-CoA: 87.1
Acetyl-CoA: 73.9
2-Methylbutyryl-CoA: 73.7
Propionyl-CoA: 63.7
CHC-CoA: 48.0
3-Methylbutyryl-CoA: 40.9
inactive: 38.1
fatty_acid: 22.8

mxaE: mmal

PKS_AT (563..859): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Ethylmalonyl-CoA: 83.3%; Malonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 176.7
Ethylmalonyl-CoA: 155.1
Methoxymalonyl-CoA: 140.4
Isobutyryl-CoA: 105.9
Malonyl-CoA: 95.9
Benzoyl-CoA: 92.5
2-Methylbutyryl-CoA: 76.3
trans-1,2-CPDA: 72.5
Acetyl-CoA: 64.6
Propionyl-CoA: 59.2
CHC-CoA: 52.6
3-Methylbutyryl-CoA: 50.2
inactive: 26.4
fatty_acid: 20.2

mxaF: pk - mmal

PKS_AT (624..920): pk

ATSignature: Isobutyryl-CoA

Top 3 matches:: Isobutyryl-CoA: 100.0%; Ethylmalonyl-CoA: 62.5%; Methylmalonyl-CoA: 62.5%

minowa: Isobutyryl-CoA

Prediction, score:

Isobutyryl-CoA: 246.1
Benzoyl-CoA: 126.0
Methylmalonyl-CoA: 101.6
Methoxymalonyl-CoA: 87.5
Ethylmalonyl-CoA: 69.8
Malonyl-CoA: 65.9
Acetyl-CoA: 58.0
3-Methylbutyryl-CoA: 51.4
trans-1,2-CPDA: 39.3
Propionyl-CoA: 38.3
CHC-CoA: 36.6
2-Methylbutyryl-CoA: 33.5
inactive: 19.3
fatty_acid: 13.5

PKS_AT (1060..1356): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Ethylmalonyl-CoA: 75.0%; Malonyl-CoA: 75.0%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 189.3
Ethylmalonyl-CoA: 171.4
Methoxymalonyl-CoA: 146.2
Isobutyryl-CoA: 112.3
Malonyl-CoA: 111.5
Benzoyl-CoA: 104.9
2-Methylbutyryl-CoA: 97.4
trans-1,2-CPDA: 85.9
Propionyl-CoA: 84.0
Acetyl-CoA: 72.7
CHC-CoA: 66.1
3-Methylbutyryl-CoA: 40.9
fatty_acid: 30.1
inactive: 22.2

Link to PARAS substrate predictor