BGC0001022 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001022 (Stigmatella aurantiaca)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS,NRPS	1	49,736	myxalamid/myxalamid A/myxalamid C/myxalamid D	NRPS:Type I+PKS	100%

BGC0001022 - Region 1 - NRPS,T1PKS

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Location: 1 - 49,736 nt. (total: 49,736 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: cds(Condensation and (AMP-binding or A-OX))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 49,737
Label		BGC0001022

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001022	1.40	NRP, Polyketide	myxalamid, myxalamid A, myxalamid C, myxalamid D	Stigmatella aurantiaca
BGC0001273	0.51	Polyketide	asperlactone	Aspergillus ochraceus
BGC0001276	0.51	Polyketide	6-methylsalicyclic acid	Aspergillus terreus
BGC0002538	0.51	Polyketide	chaetolivacine A, chaetolivacine B, chaetolivacine C	Chaetomium olivaceum
BGC0001244	0.51	Polyketide	(-)-Mellein	Parastagonospora nodorum
BGC0001275	0.50	Polyketide	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001187	0.50	NRP, Polyketide	xenolozoyenone	Glarea lozoyensis
BGC0001050	0.49	NRP, Polyketide	thalassospiramide A	Tistrella bauzanensis
BGC0002324	0.49	NRP, Polyketide	myxochelin B, myxochelin N, myxochelin O, myxochelin P, myxochelin Q, myxochelin A	Archangium violaceum
BGC0001758	0.47	NRP	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001022	1.00	NRP, Polyketide	myxalamid, myxalamid A, myxalamid C, myxalamid D	Stigmatella aurantiaca
BGC0000018	0.66	Polyketide	angolamycin	Streptomyces eurythermus
BGC0001273	0.65	Polyketide	asperlactone	Aspergillus ochraceus
BGC0001276	0.65	Polyketide	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244	0.65	Polyketide	(-)-Mellein	Parastagonospora nodorum
BGC0001275	0.64	Polyketide	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001068	0.62	Terpene, Polyketide	pyripyropene A	unidentified unclassified sequences.
BGC0002229	0.62	Polyketide	6-MSA, aculin A	Aspergillus aculeatus ATCC 16872
BGC0001954	0.61	NRP, Polyketide	wortmanamide A, wortmanamide B	Talaromyces wortmannii
BGC0000996	0.61	NRP, Polyketide	frontalamide B, frontalamide A	Streptomyces sp. SPB78

Similar known gene clusters from MIBiG 4.0

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 49736:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-ccmal) + (Me-ccmal) + (pk - ohmal - ccmal) + (Me-redmal) + (Ala)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 0 - 27822:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk - ohmal - ccmal) + (Me-redmal) + (Ala)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

mxaA

Search NORINE for peptide: strict or relaxed

AMP-binding (542..932): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: ala (Ala)

Stachelhaus prediction details:

Stachelhaus sequence:: DLFNNALTYK
Nearest Stachelhaus code(s):: Ala DLFNNALTYK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

mxaB2

PKS_AT (570..868): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Ethylmalonyl-CoA: 79.2%; Malonyl-CoA: 79.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 180.0
Ethylmalonyl-CoA: 155.3
Methoxymalonyl-CoA: 137.6
Malonyl-CoA: 112.7
Isobutyryl-CoA: 97.9
Benzoyl-CoA: 95.7
Acetyl-CoA: 89.4
2-Methylbutyryl-CoA: 88.2
CHC-CoA: 79.4
trans-1,2-CPDA: 78.8
Propionyl-CoA: 76.7
3-Methylbutyryl-CoA: 51.1
fatty_acid: 42.1
inactive: 26.1

mxaC

PKS_AT (560..858): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; Methylmalonyl-CoA: 87.5%; Ethylmalonyl-CoA: 70.8%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 142.3
Ethylmalonyl-CoA: 134.9
Malonyl-CoA: 125.2
Methoxymalonyl-CoA: 114.4
2-Methylbutyryl-CoA: 87.3
Isobutyryl-CoA: 84.5
Acetyl-CoA: 78.6
Propionyl-CoA: 65.2
Benzoyl-CoA: 63.9
trans-1,2-CPDA: 63.1
inactive: 40.7
CHC-CoA: 35.9
3-Methylbutyryl-CoA: 34.6
fatty_acid: 29.4

PKS_AT (2373..2669): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; Methylmalonyl-CoA: 75.0%; Propionyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 138.3
Methylmalonyl-CoA: 136.2
Methoxymalonyl-CoA: 126.8
Isobutyryl-CoA: 119.6
Ethylmalonyl-CoA: 101.7
trans-1,2-CPDA: 70.2
Propionyl-CoA: 70.0
2-Methylbutyryl-CoA: 65.8
Acetyl-CoA: 65.2
Benzoyl-CoA: 64.9
3-Methylbutyryl-CoA: 58.1
inactive: 54.1
CHC-CoA: 52.3
fatty_acid: 40.1

PKS_AT (3904..4197): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 91.7%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 207.9
inactive: 163.9
Methoxymalonyl-CoA: 88.9
Methylmalonyl-CoA: 82.9
Propionyl-CoA: 70.2
Isobutyryl-CoA: 50.0
Ethylmalonyl-CoA: 47.7
2-Methylbutyryl-CoA: 45.0
CHC-CoA: 41.4
Benzoyl-CoA: 32.9
trans-1,2-CPDA: 29.4
Acetyl-CoA: 20.7
3-Methylbutyryl-CoA: 20.0
fatty_acid: 17.2

mxaD

PKS_AT (551..848): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Malonyl-CoA: 87.5%; Ethylmalonyl-CoA: 79.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 175.0
Ethylmalonyl-CoA: 151.3
Methoxymalonyl-CoA: 127.0
Benzoyl-CoA: 103.3
Malonyl-CoA: 92.2
trans-1,2-CPDA: 90.5
Isobutyryl-CoA: 87.1
Acetyl-CoA: 73.9
2-Methylbutyryl-CoA: 73.7
Propionyl-CoA: 63.7
CHC-CoA: 48.0
3-Methylbutyryl-CoA: 40.9
inactive: 38.1
fatty_acid: 22.8

mxaE

PKS_AT (563..859): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Ethylmalonyl-CoA: 83.3%; Malonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 176.7
Ethylmalonyl-CoA: 155.1
Methoxymalonyl-CoA: 140.4
Isobutyryl-CoA: 105.9
Malonyl-CoA: 95.9
Benzoyl-CoA: 92.5
2-Methylbutyryl-CoA: 76.3
trans-1,2-CPDA: 72.5
Acetyl-CoA: 64.6
Propionyl-CoA: 59.2
CHC-CoA: 52.6
3-Methylbutyryl-CoA: 50.2
inactive: 26.4
fatty_acid: 20.2

mxaF

PKS_AT (624..920): pk

ATSignature: Isobutyryl-CoA

Top 3 matches:: Isobutyryl-CoA: 100.0%; Ethylmalonyl-CoA: 62.5%; Methylmalonyl-CoA: 62.5%

minowa: Isobutyryl-CoA

Prediction, score:

Isobutyryl-CoA: 246.1
Benzoyl-CoA: 126.0
Methylmalonyl-CoA: 101.6
Methoxymalonyl-CoA: 87.5
Ethylmalonyl-CoA: 69.8
Malonyl-CoA: 65.9
Acetyl-CoA: 58.0
3-Methylbutyryl-CoA: 51.4
trans-1,2-CPDA: 39.3
Propionyl-CoA: 38.3
CHC-CoA: 36.6
2-Methylbutyryl-CoA: 33.5
inactive: 19.3
fatty_acid: 13.5

PKS_AT (1060..1356): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Ethylmalonyl-CoA: 75.0%; Malonyl-CoA: 75.0%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 189.3
Ethylmalonyl-CoA: 171.4
Methoxymalonyl-CoA: 146.2
Isobutyryl-CoA: 112.3
Malonyl-CoA: 111.5
Benzoyl-CoA: 104.9
2-Methylbutyryl-CoA: 97.4
trans-1,2-CPDA: 85.9
Propionyl-CoA: 84.0
Acetyl-CoA: 72.7
CHC-CoA: 66.1
3-Methylbutyryl-CoA: 40.9
fatty_acid: 30.1
inactive: 22.2