BGC0001024 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001024 (Stigmatella aurantiaca DW4/3-1)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	NRPS,T1PKS	1	42,603	myxothiazol	NRPS:Type I+PKS:Type I	100%

BGC0001024 - Region 1 - NRPS,T1PKS

Download region GenBank file

Download region SVG

Location: 1 - 42,603 nt. (total: 42,603 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 42,604
Label		BGC0001024

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001024	2.00	NRP, Polyketide	myxothiazol	Stigmatella aurantiaca DW4/3-1
BGC0001010	0.71	NRP, Polyketide	melithiazol A	Melittangium lichenicola
BGC0000982	0.66	NRP, Polyketide	cystothiazole A	Cystobacter fuscus
BGC0001941	0.63	NRP, Polyketide	fulvuthiacene A, fulvuthiacene B	Myxococcus fulvus
BGC0001244	0.61	Polyketide	(-)-Mellein	Parastagonospora nodorum
BGC0001273	0.60	Polyketide	asperlactone	Aspergillus ochraceus
BGC0001275	0.60	Polyketide	6-methylsalicyclic acid	Glarea lozoyensis
BGC0000041	0.59	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0001068	0.56	Terpene, Polyketide	pyripyropene A	unidentified unclassified sequences.
BGC0001012	0.52	NRP, Polyketide	meridamycin	Streptomyces violaceusniger

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001024	1.00	NRP, Polyketide	myxothiazol	Stigmatella aurantiaca DW4/3-1
BGC0001010	0.69	NRP, Polyketide	melithiazol A	Melittangium lichenicola
BGC0000982	0.66	NRP, Polyketide	cystothiazole A	Cystobacter fuscus
BGC0001941	0.65	NRP, Polyketide	fulvuthiacene A, fulvuthiacene B	Myxococcus fulvus
BGC0001244	0.64	Polyketide	(-)-Mellein	Parastagonospora nodorum
BGC0001273	0.64	Polyketide	asperlactone	Aspergillus ochraceus
BGC0001275	0.63	Polyketide	6-methylsalicyclic acid	Glarea lozoyensis
BGC0000041	0.63	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0001068	0.61	Terpene, Polyketide	pyripyropene A	unidentified unclassified sequences.
BGC0001012	0.60	NRP, Polyketide	meridamycin	Streptomyces violaceusniger

Similar known gene clusters from MIBiG 4.0

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 42603:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-ccmal) + (Cys) + (Cys - ccmal) + (OMe-Me-ohmal) + (OMe-mal) + (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

mtaB

PKS_AT (558..855): pk

ATSignature: 3-Methylbutyryl-CoA

Top 3 matches:: 3-Methylbutyryl-CoA: 100.0%

minowa: 3-Methylbutyryl-CoA

Prediction, score:

3-Methylbutyryl-CoA: 272.9
Isobutyryl-CoA: 79.5
Methylmalonyl-CoA: 78.6
Methoxymalonyl-CoA: 73.1
Benzoyl-CoA: 58.0
2-Methylbutyryl-CoA: 57.6
Propionyl-CoA: 48.6
Acetyl-CoA: 34.3
trans-1,2-CPDA: 33.5
Malonyl-CoA: 22.2
CHC-CoA: 18.2
inactive: 18.1
fatty_acid: 14.1
Ethylmalonyl-CoA: -2.7

PKS_AT (986..1281): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; Methylmalonyl-CoA: 66.7%; Ethylmalonyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 124.9
Methylmalonyl-CoA: 108.4
Methoxymalonyl-CoA: 92.3
Ethylmalonyl-CoA: 70.8
Isobutyryl-CoA: 55.9
Propionyl-CoA: 54.2
trans-1,2-CPDA: 49.3
Benzoyl-CoA: 42.5
fatty_acid: 39.5
inactive: 37.1
3-Methylbutyryl-CoA: 32.5
2-Methylbutyryl-CoA: 29.8
CHC-CoA: 24.7
Acetyl-CoA: 23.1

PKS_AT (2758..3053): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Isobutyryl-CoA: 79.2%; Malonyl-CoA: 66.7%

minowa: Isobutyryl-CoA

Prediction, score:

Isobutyryl-CoA: 153.5
Methylmalonyl-CoA: 119.6
Malonyl-CoA: 104.5
Methoxymalonyl-CoA: 95.7
Ethylmalonyl-CoA: 61.4
Benzoyl-CoA: 60.6
inactive: 42.8
Propionyl-CoA: 31.4
Acetyl-CoA: 28.3
3-Methylbutyryl-CoA: 25.6
2-Methylbutyryl-CoA: 25.5
trans-1,2-CPDA: 25.4
fatty_acid: 18.8
CHC-CoA: 16.9

mtaC

Search NORINE for peptide: strict or relaxed

AMP-binding (482..879): Cys

nrpys: Cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: DLYNMSLIWK
Nearest Stachelhaus code(s):: Cys DLYNMSLIWK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

mtaD

Search NORINE for peptide: strict or relaxed

AMP-binding (538..937): Cys

nrpys: Cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: DLYNLSLIWK
Nearest Stachelhaus code(s):: Cys DLYNLSLIWK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

PKS_AT (1987..2281): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 70.8%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 172.0
inactive: 134.0
Methoxymalonyl-CoA: 72.8
Methylmalonyl-CoA: 65.3
Propionyl-CoA: 38.9
Isobutyryl-CoA: 29.4
2-Methylbutyryl-CoA: 25.5
Ethylmalonyl-CoA: 24.5
CHC-CoA: 22.4
Benzoyl-CoA: 21.9
Acetyl-CoA: 18.3
trans-1,2-CPDA: 14.0
fatty_acid: 10.0
3-Methylbutyryl-CoA: 6.4

mtaE

PKS_AT (576..873): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Ethylmalonyl-CoA: 79.2%; Methoxymalonyl-CoA: 79.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 178.4
Ethylmalonyl-CoA: 146.8
Methoxymalonyl-CoA: 139.4
Isobutyryl-CoA: 128.0
Benzoyl-CoA: 111.0
Malonyl-CoA: 89.0
Acetyl-CoA: 88.5
2-Methylbutyryl-CoA: 84.5
trans-1,2-CPDA: 76.1
Propionyl-CoA: 70.1
CHC-CoA: 66.0
3-Methylbutyryl-CoA: 45.7
fatty_acid: 39.0
inactive: 28.0

mtaF

PKS_AT (566..860): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 70.8%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 172.0
inactive: 134.0
Methoxymalonyl-CoA: 72.8
Methylmalonyl-CoA: 65.3
Propionyl-CoA: 38.9
Isobutyryl-CoA: 29.4
2-Methylbutyryl-CoA: 25.5
Ethylmalonyl-CoA: 24.5
CHC-CoA: 22.4
Benzoyl-CoA: 21.9
Acetyl-CoA: 18.3
trans-1,2-CPDA: 14.0
fatty_acid: 10.0
3-Methylbutyryl-CoA: 6.4

mtaG

A-OX (547..1308): X