BGC0001028: nostopeptolide A2 biosynthetic gene cluster from Nostoc sp. GSV224
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Location: 1 - 40,989 nt. (total: 40,989 nt).
This entry is originally from NCBI GenBank AF204805.2.

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
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Gene details
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General
Compounds
Genes
Polyketide
NRP
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0001028
Short description nostopeptolide A2 biosynthetic gene cluster from Nostoc sp. GSV224
Status Minimal annotation: no
A minimal annotation only contains information on the BGC loci and one or more linked chemical product(s)

Completeness: complete
Whether the loci encodes everything needed for the pathway producing the compound(s)
Biosynthetic class(es)
  • NRP (Cyclic depsipeptide)
  • Polyketide
Loci NCBI GenBank: AF204805.2
Compounds
  • nostopeptolide A2
Species Nostoc sp. GSV224 [taxonomy]
References
Chemical products information
nostopeptolide A2
Copy SMILES
C52H78N10O14
Chemical database entries
NPAtlas
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • AAF15891.2
  • nosA
 1370 - 14509 (+) nosA
  • Scaffold biosynthesis
Activity assay
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  • AAF15892.2
  • nosB
 14506 - 18240 (+) nosB
  • Scaffold biosynthesis
Sequence-based prediction
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  • AAF17280.1
  • nosC
 18312 - 28265 (+) nosC
  • Scaffold biosynthesis
Activity assay
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  • AAF17281.1
  • nosD
 28262 - 35614 (+) nosD
  • Scaffold biosynthesis
Activity assay
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  • AAF17282.1
 35820 - 36617 (+) Unknown
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  • AAF17283.1
  • nosE
 36665 - 37771 (+) nosE
  • Precursor biosynthesis
Sequence-based prediction
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  • AAF17284.1
  • nosF
 37811 - 38632 (+) nosF
  • Precursor biosynthesis
Sequence-based prediction
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  • AAF17285.1
  • nosG
 38734 - 40725 (+) nosG
  • Transport
Sequence-based prediction
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Polyketide-specific information
Subclass Unknown
Cyclic? no
Polyketide-synthases
Genes Properties Modules
nosB Synthase subclass:
Module 5
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: nosB
Core domains: Ketosynthase, Acyltransferase, Thiolation (ACP/PCP)
NRP-specific information
Subclass Cyclic depsipeptide
Cyclic? yes
Release type
  • Macrolactonization
Thioesterase genes
  • nosD (Type I)
NRP-synthases
Gene Modules
nosA
Module 1
Specificity: isoleucine
Evidence for specificity:
  • Activity assay
Condensation domain type: LCL
Module 2
Specificity: serine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: LCL
Module 3
Specificity: 4S-methylproline
Evidence for specificity:
  • Structure-based inference
Condensation domain type: LCL
Module 4
Specificity: leucine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: LCL
nosC
Module 6
Specificity: leucine
Evidence for specificity:
  • Activity assay
Condensation domain type: LCL
Module 7
Specificity: glycine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: LCL
Module 8
Specificity: asparagine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: LCL
nosD
Module 10
Specificity: proline
Evidence for specificity:
  • Structure-based inference
Condensation domain type: LCL
Module 9
Specificity: tyrosine / D-tyrosine
Evidence for specificity:
  • ATP-PPi exchange assay
Condensation domain type: LCL
Annotation changelog
MIBiG version Submitter Notes
1.0
  • Hidden contributor (ID: N52IJH5LHPQ7BFWMKQOBW6U7, no GDPR consent given).
  • Submitted
2.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Migrated from v1.4
  • Updated compound(s) information (NPAtlas curation)
3.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Corrected NRP module activity
  • Removed duplicate gene name
  • Removed ketoreductase stereochemistry annotation from modules without ketoreductases
  • Updated bioactivity data
  • Updated NRP substrate specificities
3.1
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Update chemical activity to schema version 2.11
Detailed domain annotation
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