BGC0001028 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001028 (Nostoc sp. GSV224)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	NRPS,T1PKS	1	40,989	nostopeptolide A2	NRPS:Type I+PKS	100%

BGC0001028 - Region 1 - NRPS,T1PKS

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Location: 1 - 40,989 nt. (total: 40,989 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 40,990
Label		BGC0001028

Detailed domain annotation

Selected features only

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Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001028	1.94	NRP, Polyketide	nostopeptolide A2	Nostoc sp. GSV224
BGC0002061	1.31	NRP, Polyketide	nostopeptolide A1, nostopeptolide 1052	Nostoc punctiforme PCC 73102
BGC0001876	0.77	NRP	thalassospiramide A	Thalassospira sp. CNJ-328
BGC0001844	0.75	NRP	holrhizin	Paraburkholderia rhizoxinica HKI 454
BGC0001758	0.74	NRP	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0002071	0.72	NRP	virginiafactin A, virginiafactin B, virginiafactin C, virginiafactin D	Pseudomonas sp. QS1027
BGC0002135	0.71	NRP	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001629	0.70	NRP	jessenipeptin	Pseudomonas sp. QS1027
BGC0001133	0.69	NRP	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0001833	0.68	NRP	icosalide A, icosalide B	Burkholderia gladioli

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001028	1.00	NRP, Polyketide	nostopeptolide A2	Nostoc sp. GSV224
BGC0002061	0.88	NRP, Polyketide	nostopeptolide A1, nostopeptolide 1052	Nostoc punctiforme PCC 73102
BGC0001876	0.71	NRP	thalassospiramide A	Thalassospira sp. CNJ-328
BGC0001844	0.70	NRP	holrhizin	Paraburkholderia rhizoxinica HKI 454
BGC0001758	0.70	NRP	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0002071	0.69	NRP	virginiafactin A, virginiafactin B, virginiafactin C, virginiafactin D	Pseudomonas sp. QS1027
BGC0002135	0.68	NRP	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001131	0.68	NRP	ambactin	Xenorhabdus miraniensis
BGC0001629	0.68	NRP	jessenipeptin	Pseudomonas sp. QS1027
BGC0001133	0.68	NRP	taxlllaid A	Xenorhabdus bovienii SS-2004

Similar known gene clusters from MIBiG 4.0

Module view

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 40989:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Ile - Ser - X - Leu) + (mal) + (Leu - Gly - Asn) + (X - Pro)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

nosA

Search NORINE for peptide: strict or relaxed

AMP-binding (484..898): Ile

nrpys: Ile

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: ile (Ile)

Stachelhaus prediction details:

Stachelhaus sequence:: DAFFLGVTFK
Nearest Stachelhaus code(s):: Ile DAFFLGVTFK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1568..1987): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: DVWHISLIDK
Nearest Stachelhaus code(s):: Ser DVWHISLIDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2649..3075): X

nrpys: 4Me-Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: pro (Pro)
Single AA prediction:: pro (Pro)

Stachelhaus prediction details:

Stachelhaus sequence:: DVQFIAHLAK
Nearest Stachelhaus code(s):: 4Me-Pro DVQFIAHLAK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (3740..4158): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: leu (Leu)

Stachelhaus prediction details:

Stachelhaus sequence:: DAWFLGNVVK
Nearest Stachelhaus code(s):: Leu DAWFLGNVVK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

nosB

PKS_AT (543..839): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 87.5%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 167.7
inactive: 122.6
Methoxymalonyl-CoA: 71.5
Methylmalonyl-CoA: 60.2
Propionyl-CoA: 43.9
Isobutyryl-CoA: 32.6
2-Methylbutyryl-CoA: 29.7
Benzoyl-CoA: 28.9
Ethylmalonyl-CoA: 26.8
CHC-CoA: 22.5
Acetyl-CoA: 18.8
trans-1,2-CPDA: 8.7
fatty_acid: 8.5
3-Methylbutyryl-CoA: 0.0

nosC

Search NORINE for peptide: strict or relaxed

AMP-binding (537..947): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: leu (Leu)

Stachelhaus prediction details:

Stachelhaus sequence:: DAWFLGNVVK
Nearest Stachelhaus code(s):: Leu DAWFLGNVVK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1623..2048): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: DILQLGLIWK
Nearest Stachelhaus code(s):: Gly DILQLGLIWK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2710..3121): Asn

nrpys: Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: DATKVGEVGK
Nearest Stachelhaus code(s):: Asn DATKVGEVGK (100% 8Å match); OH-Asn or Asn DATKVGEVGK (85% 8Å match)
Stachelhaus code match:: 100% (strong)

nosD

Search NORINE for peptide: strict or relaxed

AMP-binding (469..879): X

nrpys: Tyr, Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Polar aromatic ring (Tyr, bOH-Tyr)
Single AA prediction:: tyr (Tyr)

Stachelhaus prediction details:

Stachelhaus sequence:: DASTIAAVCK
Nearest Stachelhaus code(s):: Tyr or Pro DASTIAAVCK (100% 8Å match); Tyr DASTIAAVCK (88% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1541..1956): Pro

nrpys: Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: pro (Pro)
Single AA prediction:: pro (Pro)

Stachelhaus prediction details:

Stachelhaus sequence:: DVQFIAHVIK
Nearest Stachelhaus code(s):: Pro DVQFIAHVIK (100% 8Å match); Tyr DVQFIAHVIK (97% 8Å match)
Stachelhaus code match:: 100% (strong)