BGC0001028: nostopeptolide A2 biosynthetic gene cluster from Nostoc sp. GSV224
Shows the layout of the region, marking coding sequences and areas of interest. Clicking a gene will select it and show any relevant details. Clicking an area feature (e.g. a candidate cluster) will select all coding sequences within that area. Double clicking an area feature will zoom to that area. Multiple genes and area features can be selected by clicking them while holding the Ctrl key.
Location: 1 - 40,989 nt. (total: 40,989 nt).
This entry is originally from NCBI GenBank AF204805.2.

Legend:

core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
resistance genes
other genes
reset zoomreset view
zoomzoom to selection
Gene details
Shows details of the most recently selected gene, including names, products, location, and other annotations.
Select a gene to view the details available for it
General
Compounds
Genes
Polyketide
NRP
History
KnownClusterBlast
NRPS/PKS domains
General information about the BGC
MIBiG accession BGC0001028
Short description nostopeptolide A2 biosynthetic gene cluster from Nostoc sp. GSV224
Status Minimal annotation: no
A minimal annotation only contains information on the BGC loci and one or more linked chemical product(s)

Completeness: complete
Whether the loci encodes everything needed for the pathway producing the compound(s)
Biosynthetic class(es)
  • NRP (Cyclic depsipeptide)
  • Polyketide
Loci NCBI GenBank: AF204805.2
Compounds
  • nostopeptolide A2
Species Nostoc sp. GSV224 [taxonomy]
References
Chemical products information
nostopeptolide A2
Copy SMILES
C52H78N10O14
Chemical database entries
NPAtlas
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • AAF15891.2
  • nosA
1370 - 14509 (+) nosA
  • Scaffold biosynthesis
Activity assay
copy AA seq
copy Nt seq
  • AAF15892.2
  • nosB
14506 - 18240 (+) nosB
  • Scaffold biosynthesis
Sequence-based prediction
copy AA seq
copy Nt seq
  • AAF17280.1
  • nosC
18312 - 28265 (+) nosC
  • Scaffold biosynthesis
Activity assay
copy AA seq
copy Nt seq
  • AAF17281.1
  • nosD
28262 - 35614 (+) nosD
  • Scaffold biosynthesis
Activity assay
copy AA seq
copy Nt seq
  • AAF17282.1
35820 - 36617 (+) Unknown
copy AA seq
copy Nt seq
  • AAF17283.1
  • nosE
36665 - 37771 (+) nosE
  • Precursor biosynthesis
Sequence-based prediction
copy AA seq
copy Nt seq
  • AAF17284.1
  • nosF
37811 - 38632 (+) nosF
  • Precursor biosynthesis
Sequence-based prediction
copy AA seq
copy Nt seq
  • AAF17285.1
  • nosG
38734 - 40725 (+) nosG
  • Transport
Sequence-based prediction
copy AA seq
copy Nt seq
Polyketide-specific information
Subclass Unknown
Cyclic? no
Polyketide-synthases
Genes Properties Modules
AAF15892.2 Synthase subclass:
Module 5
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: AAF15892.2
Core domains: Ketosynthase, Acyltransferase, Thiolation (ACP/PCP)
NRP-specific information
Subclass Cyclic depsipeptide
Cyclic? yes
Release type
  • Macrolactonization
Thioesterase genes
  • AAF17281.1 (Type I)
NRP-synthases
Gene Modules
AAF15891.2
Module 1
Specificity: Isoleucine
Evidence for specificity:
  • Activity assay
Condensation domain type: LCL
Module 2
Specificity: Serine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: LCL
Module 3
Specificity: Proline
Evidence for specificity:
  • Structure-based inference
Condensation domain type: LCL
Module 4
Specificity: Leucine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: LCL
AAF17280.1
Module 6
Specificity: Leucine
Evidence for specificity:
  • Activity assay
Condensation domain type: LCL
Module 7
Specificity: Glycine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: LCL
Module 8
Specificity: Asparigine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: LCL
AAF17281.1
Module 10
Specificity: Proline
Evidence for specificity:
  • Structure-based inference
Condensation domain type: LCL
Module 9
Specificity: Tyrosine
Evidence for specificity:
  • Activity assay
Condensation domain type: LCL
Annotation changelog
MIBiG version Submitter Notes
1.0
  • Hidden contributor (ID: N52IJH5LHPQ7BFWMKQOBW6U7, no GDPR consent given).
  • Submitted
2.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Migrated from v1.4
  • Updated compound(s) information (NPAtlas curation)
Similar known gene clusters
Shows clusters from the MiBIG database that are similar to the current region. Genes marked with the same colour are interrelated. White genes have no relationship.
Click on reference genes to show details of similarities to genes within the current region.
Click on an accession to open that entry in the MiBIG database.
Detailed domain annotation
Shows NRPS- and PKS-related domains for each feature that contains them. Click on each domain for more information about the domain's location, consensus monomer prediction, and other details.
A glossary is available here.
Selected features only
Show module domains