BGC0001030 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001030 (Penicillium nordicum)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	NRPS,T1PKS	1	22,759	High	ochratoxin A	NRPS:Type I+PKS

BGC0001030 - Region 1 - NRPS,T1PKS

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Location: 1 - 22,759 nt. (total: 22,759 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 22,760
Label		BGC0001030

Tailoring functions

Oxidoreductases: oxygenase

AUS29493.1: cytochrome P450

smcogs: SMCOG1034: cytochrome P450

Transferases: acylase halogenase

AUS29491.1: conventional flavin-dependent halogenase

halogenases: conventional flavin-dependent halogenase
smcogs: SMCOG1119: halogenase

AUS29495.1: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

Ligases: ligase

AUS29494.1: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001030.3	1.87	NRPS, PKS	ochratoxin A	Penicillium nordicum
BGC0002608.2	1.40	NRPS, PKS	ochratoxin A	Aspergillus steynii
BGC0002607.2	1.14	NRPS, PKS	ochratoxin A	Aspergillus westerdijkiae
BGC0002606.2	1.05	NRPS, PKS	ochratoxin A	Aspergillus niger
BGC0002605.2	1.04	NRPS, PKS	ochratoxin A	Aspergillus carbonarius
BGC0002598.2	0.95	PKS	ochratoxin A	Aspergillus westerdijkiae
BGC0002191.2	0.89	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001249.5	0.85	NRPS	dimethylcoprogen	Alternaria alternata
BGC0002164.2	0.70	NRPS	peramine	Epichloe festucae
BGC0001261.3	0.69	NRPS	AM-toxin	Alternaria alternata

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001030.3	0.98	NRPS, PKS	ochratoxin A	Penicillium nordicum
BGC0002608.2	0.89	NRPS, PKS	ochratoxin A	Aspergillus steynii
BGC0002607.2	0.82	NRPS, PKS	ochratoxin A	Aspergillus westerdijkiae
BGC0002606.2	0.80	NRPS, PKS	ochratoxin A	Aspergillus niger
BGC0002605.2	0.80	NRPS, PKS	ochratoxin A	Aspergillus carbonarius
BGC0002598.2	0.77	PKS	ochratoxin A	Aspergillus westerdijkiae
BGC0002191.2	0.75	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001249.5	0.74	NRPS	dimethylcoprogen	Alternaria alternata
BGC0002164.2	0.68	NRPS	peramine	Epichloe festucae
BGC0001261.3	0.67	NRPS	AM-toxin	Alternaria alternata

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 22759:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Val) + (Me-pk)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

AUS29494.1: Val - Ala

Search NORINE for peptide: strict or relaxed

AMP-binding (202..596): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: TVCWTGSGWK
Nearest Stachelhaus code(s):: Asn DVAFTGSIWK (44% 8Å match)
Stachelhaus code match:: 60% (weak)

AMP-binding (1270..1663): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: pro (Pro)
Single AA prediction:: ala (Ala)

Stachelhaus prediction details:

Stachelhaus sequence:: DAIWCMAVLK
Nearest Stachelhaus code(s):: Tyr DAIDVGAVGK (53% 8Å match); Val DAWMFAAVLK (44% 8Å match)
Stachelhaus code match:: 60% (weak)

AUS29495.1: pk

PKS_AT (570..871): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; Methylmalonyl-CoA: 66.7%; Ethylmalonyl-CoA: 58.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 60.4
Methoxymalonyl-CoA: 55.1
Malonyl-CoA: 40.7
Propionyl-CoA: 30.6
Ethylmalonyl-CoA: 28.9
fatty_acid: 21.8
CHC-CoA: 12.2
2-Methylbutyryl-CoA: 11.0
Isobutyryl-CoA: 10.0
Benzoyl-CoA: 9.5
inactive: 9.2
3-Methylbutyryl-CoA: 9.0
trans-1,2-CPDA: 0.0
Acetyl-CoA: 0.0

Link to PARAS substrate predictor