BGC0001033 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001033 (Paenibacillus larvae subsp. larvae DSM 25430)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	NRPS,T1PKS	1	58,753	paenilamicin/paenilamicin A2/paenilamicin B1/paenilamicin B2	NRPS:Type I+PKS	100%

BGC0001033 - Region 1 - NRPS,T1PKS

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Location: 1 - 58,753 nt. (total: 58,753 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 58,754
Label		BGC0001033

Detailed domain annotation

Selected features only

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Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001033	1.90	NRP, Polyketide	paenilamicin, paenilamicin A2, paenilamicin B1, paenilamicin B2	Paenibacillus larvae subsp. larvae DSM 25430
BGC0000426	0.84	NRP	sevadicin	Paenibacillus larvae
BGC0002071	0.77	NRP	virginiafactin A, virginiafactin B, virginiafactin C, virginiafactin D	Pseudomonas sp. QS1027
BGC0002135	0.67	NRP	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0002518	0.67	NRP	syringafactin A, syringafactin C	Pseudomonas sp. SZ57
BGC0001023	0.66	NRP, Polyketide	myxochromide S1	Stigmatella aurantiaca
BGC0001133	0.66	NRP	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0002437	0.66	NRP	thermoactinoamide A	Thermoactinomyces sp. AS95
BGC0001876	0.64	NRP	thalassospiramide A	Thalassospira sp. CNJ-328
BGC0002017	0.64	NRP	potensibactin	Nocardiopsis potens DSM 45234

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001033	1.00	NRP, Polyketide	paenilamicin, paenilamicin A2, paenilamicin B1, paenilamicin B2	Paenibacillus larvae subsp. larvae DSM 25430
BGC0000426	0.75	NRP	sevadicin	Paenibacillus larvae
BGC0002071	0.71	NRP	virginiafactin A, virginiafactin B, virginiafactin C, virginiafactin D	Pseudomonas sp. QS1027
BGC0002437	0.68	NRP	thermoactinoamide A	Thermoactinomyces sp. AS95
BGC0001023	0.67	NRP, Polyketide	myxochromide S1	Stigmatella aurantiaca
BGC0001133	0.67	NRP	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0002135	0.67	NRP	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0002518	0.67	NRP	syringafactin A, syringafactin C	Pseudomonas sp. SZ57
BGC0001876	0.66	NRP	thalassospiramide A	Thalassospira sp. CNJ-328
BGC0002017	0.65	NRP	potensibactin	Nocardiopsis potens DSM 45234

Similar known gene clusters from MIBiG 4.0

Module view

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 58753:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - ohmal) + (ohmal - D-Ala) + (D-Lys) + (Ser) + (ohmal) + (Gly) + (D-Asn)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ERIC2_c18040

AMP-binding (470..858): X

nrpys: Lys, Arg

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DPEDIGGVEK
Nearest Stachelhaus code(s):: Lys or Arg DPEDIGGVEK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

PKS_AT (1575..1866): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 151.8
inactive: 91.5
Methoxymalonyl-CoA: 62.7
Methylmalonyl-CoA: 48.8
Propionyl-CoA: 28.8
fatty_acid: 25.1
2-Methylbutyryl-CoA: 24.2
CHC-CoA: 21.3
Benzoyl-CoA: 18.9
Isobutyryl-CoA: 16.7
Acetyl-CoA: 16.3
Ethylmalonyl-CoA: 13.0
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

ERIC2_c18050

Search NORINE for peptide: strict or relaxed

PKS_AT (529..816): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; 2-Rhyd-Malonyl-CoA: 62.5%; inactive: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 136.6
Methoxymalonyl-CoA: 75.6
inactive: 67.7
Methylmalonyl-CoA: 56.3
Propionyl-CoA: 38.9
Ethylmalonyl-CoA: 30.8
Isobutyryl-CoA: 24.9
2-Methylbutyryl-CoA: 20.2
CHC-CoA: 17.2
3-Methylbutyryl-CoA: 11.0
trans-1,2-CPDA: 9.7
Acetyl-CoA: 9.7
fatty_acid: 0.0
Benzoyl-CoA: 0.0

AMP-binding (1971..2365): D-Ala

nrpys: D-Ala

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKVNEVGK
Nearest Stachelhaus code(s):: D-Ala DLTKVNEVGK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

ERIC2_c18060

AMP-binding (845..1135): X

nrpys: Dpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DIWEVTADDK
Nearest Stachelhaus code(s):: Dpr DIWEVTADDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

ERIC2_c18070

Search NORINE for peptide: strict or relaxed

AMP-binding (276..683): Lys

nrpys: Lys

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DMEDVGSVDK
Nearest Stachelhaus code(s):: Lys DMEDVGSVDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

ERIC2_c18080

Search NORINE for peptide: strict or relaxed

AMP-binding (482..889): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: DVWHFSLVDK
Nearest Stachelhaus code(s):: Ser DVWHFSLVDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

ERIC2_c18090

PKS_AT (533..828): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; inactive: 75.0%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 141.9
inactive: 110.5
Methoxymalonyl-CoA: 71.8
Methylmalonyl-CoA: 54.8
Propionyl-CoA: 32.4
Ethylmalonyl-CoA: 28.0
trans-1,2-CPDA: 22.5
Acetyl-CoA: 22.4
fatty_acid: 19.6
Isobutyryl-CoA: 17.1
CHC-CoA: 12.9
2-Methylbutyryl-CoA: 11.0
Benzoyl-CoA: 10.9
3-Methylbutyryl-CoA: 0.0

ERIC2_c18110

Search NORINE for peptide: strict or relaxed

AMP-binding (280..545): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: IPVLVL-VSK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

AMP-binding (840..1130): X

nrpys: Dpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DIWEVTADDK
Nearest Stachelhaus code(s):: Dpr DIWEVTADDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1778..2204): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DIFHMGMIWK
Nearest Stachelhaus code(s):: Gly DIFHMGMIWK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

ERIC2_c18170

Search NORINE for peptide: strict or relaxed

AMP-binding (461..858): Asn

nrpys: Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKIGEVGK
Nearest Stachelhaus code(s):: Asn DLTKIGEVGK (100% 8Å match)
Stachelhaus code match:: 100% (strong)