Genomic loci for this biosynthetic pathway

Cluster Type From To
The following clusters are from record BGC0001034.1:
Cluster 1NRP / Polyketide161485

BGC0001034, pellasoren biosynthetic gene cluster from Sorangium cellulosum. Locus 1. Full MIBiG entry.

Chemical compounds

Compound: Pellasoren
SMILES string: Copy to clipboard
Molecular formula: C26H41NO4
Exact molecular mass: 432.31084 Da ([M+H]+)
Molecular activity: Cytotoxic

Class-specific details

Biosynthetic class(es):
NRP / Polyketide
Other (linear)
Thioesterase type:
Type I
Release / cyclization type:
Macrolactonization

Nonribosomal peptide synthetases:
PelA (ctg1_orf00037)
Module 2
A specificity: Alanine
Evidence for specificity: Structure-based inference
C domain subtype: LCL
Polyketide subclass:
Other (linear)
Polyketide synthase subclass:
Modular type I
Starter unit:
Propionyl-CoA
Thioesterase type:
Type I
Release / cyclization type:
Macrolactonization

Modular polyketide synthases:
pelA
Module 1
AT specificity: Methoxymalonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: B-group
Module 2
AT specificity: Unknown
Evidence for specificity: Unknown
KR stereochemistry: Inactive
Module is skipped
Evidence for skipping: Structure-based inference

pelB
Module 3
AT specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: B-group

pelC
Module 4
AT specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: B-group

pelD
Module 5
AT specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: B-group
Module 6
AT specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: B-group

pelE
Module 7
AT specificity: Methylmalonyl-CoA
Evidence for specificity: Unknown
KR stereochemistry: B-group

pelF
Module 8
AT specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
KR stereochemistry: B-group

Gene cluster description

pellasoren (BGC0001034). Gene Cluster 1. Biosynthetic class = NRP/Polyketide. GenBank HE616533, positions 708-61485. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Domain annotation

Homologous known gene clusters

General MIBiG information on this cluster

Complete gene cluster sequence?complete
Evidence for cluster-compound connection:Knock-out studies
MIxS-compliance:No
Comments:DH of module 7 is inactive as inferred from the structure of pellasoren. Active site alignment showed two aa being different to the other active DHs within the cluster. I don't know how to mark this DH as inactive.
Contact for this cluster:Thomas Hoffmann (Helmholtz Institute for Pharmaceutical Research Saarland (HIPS))

Literature references

1. Jahns C et al. (2012) Pellasoren: structure elucidation, biosynthesis, and total synthesis of a cytotoxic secondary metabolite from Sorangium cellulosum. Angew Chem Int Ed Engl 51(21):5239-43. doi: 10.1002/anie.201200327.