BGC0001035 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001035 (Glarea lozoyensis ATCC 20868)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS,NRPS,betalactone	1	115,835	High	pneumocandin B0/pneumocandin A0	PKS+NRPS:Type I

BGC0001035 - Region 1 - NRPS,T1PKS,betalactone

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Location: 1 - 115,835 nt. (total: 115,835 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
betalactone: (HMGL-like and AMP-binding)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 115,836
Label		BGC0001035

Tailoring functions

Oxidoreductases: oxygenase

GLAREA_10030: cytochrome P450

smcogs: SMCOG1034: cytochrome P450

GLAREA_10031: cytochrome P450

smcogs: SMCOG1034: cytochrome P450

Ligases: ligase synthase

GLAREA_10034: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

GLAREA_10035: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

GLAREA_10039: 2-isopropylmalate synthase

smcogs: SMCOG1271: 2-isopropylmalate synthase

GLAREA_10043: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Translocases: permease

GLAREA_10026: phenylalanine-specific permease

smcogs: SMCOG1038: phenylalanine-specific permease

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001035.5	1.49	NRPS, PKS	pneumocandin B0, pneumocandin A0	Glarea lozoyensis ATCC 20868
BGC0002191.2	0.91	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001244.3	0.64	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0000107.3	0.64	PKS	naphthopyrone	Aspergillus nidulans FGSC A4
BGC0001273.3	0.64	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.64	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001275.3	0.64	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0002192.3	0.62	PKS	FR901512	fungal sp. No.14919
BGC0001254.3	0.59	PKS	ACT-Toxin II	Alternaria alternata
BGC0002286.3	0.59	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001035.5	0.99	NRPS, PKS	pneumocandin B0, pneumocandin A0	Glarea lozoyensis ATCC 20868
BGC0002191.2	0.76	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0002135.2	0.73	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001758.4	0.72	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001135.5	0.71	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001166.5	0.71	NRPS	HC-toxin	Alternaria jesenskae
BGC0001833.4	0.71	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001261.3	0.71	NRPS	AM-toxin	Alternaria alternata
BGC0001128.4	0.70	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.70	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 20469 - 103446:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-pk) + (X - Thr - X - X - X - X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 20469 - 68499:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-pk) + (X - Thr - X - X - X - X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 30153 - 91843:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-pk) + (X - Thr - X - X - X - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

GLAREA_10034: pk

PKS_AT (602..906): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; Propionyl-CoA: 62.5%; 2-Methylbutyryl-CoA: 62.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 83.2
Methoxymalonyl-CoA: 77.0
Malonyl-CoA: 56.6
Ethylmalonyl-CoA: 46.5
Propionyl-CoA: 46.1
Benzoyl-CoA: 37.2
Isobutyryl-CoA: 37.0
2-Methylbutyryl-CoA: 36.3
trans-1,2-CPDA: 26.4
3-Methylbutyryl-CoA: 26.4
fatty_acid: 25.9
CHC-CoA: 25.5
inactive: 7.4
Acetyl-CoA: 6.0

GLAREA_10035: X - Thr - X - X - X - X

Search NORINE for peptide: strict or relaxed

AMP-binding (674..1052): X

nrpys: dhOrn

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVCEASSIVK
Nearest Stachelhaus code(s):: dhOrn DVCEASSIVK (100% 8Å match); Orn DVCEASSIVK (88% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1757..2154): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAQTAVAIHK
Nearest Stachelhaus code(s):: Thr DAQTAVAIHK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2848..3245): X

nrpys: 4OH-Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVSSATTVCK
Nearest Stachelhaus code(s):: 4OH-Pro DVSSATTVCK (100% 8Å match); Pro DVSSATTVCK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (3919..4320): X

nrpys: dohHty

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DGEAVGCIFK
Nearest Stachelhaus code(s):: dohHty DGEAVGCIFK (100% 8Å match); ohHty DGEAVGCIFK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (5012..5414): X

nrpys: bOH-Gln

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAQNIASINK
Nearest Stachelhaus code(s):: bOH-Gln DAQNIASINK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (6110..6507): X

nrpys: 3OH-Pro, 3S-oh-4S-mePro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DNTMITAMSK
Nearest Stachelhaus code(s):: 3OH-Pro or 3S-oh-4S-mePro DNTMITAMSK (100% 8Å match); 3S-oh-4S-mePro DNTMITAMSK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

GLAREA_10043: X

AMP-binding (39..463): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: GLQYIGGVCK
Nearest Stachelhaus code(s):: Lin AYVYIGGVFK (47% 8Å match); Leu or Phe DAWTIGGVCK (29% 8Å match)
Stachelhaus code match:: 60% (weak)

Link to PARAS substrate predictor