BGC0001035 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001035 (Glarea lozoyensis ATCC 20868)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS,NRPS,betalactone	1	115,835	pneumocandin B0/pneumocandin A0	PKS+NRPS:Type I	100%

BGC0001035 - Region 1 - NRPS,T1PKS,betalactone

Download region GenBank file

Download region SVG

Location: 1 - 115,835 nt. (total: 115,835 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: cds(Condensation and (AMP-binding or A-OX))
betalactone: (HMGL-like and AMP-binding)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 115,836
Label		BGC0001035

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001035	1.54	Polyketide, NRP	pneumocandin B0, pneumocandin A0	Glarea lozoyensis ATCC 20868
BGC0002525	0.76	Polyketide	fusarubin, 1233A, 1233B, NG-391, lucilactaene	Fusarium sp.
BGC0000146	0.67	Polyketide	solanapyrone D	Alternaria solani
BGC0002191	0.65	Polyketide	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0002515	0.63	Polyketide	solanapyrone A	Ascochyta rabiei
BGC0001899	0.62	Polyketide	abscisic acid	Leptosphaeria maculans JN3
BGC0001909	0.62	Polyketide	strobilurin A	Strobilurus tenacellus
BGC0000012	0.61	Polyketide	alternapyrone	Alternaria solani
BGC0001252	0.60	Polyketide	UNII-YC2Q1O94PT	Alternaria alternata
BGC0002194	0.60	Polyketide	epipyrone A	Epicoccum nigrum

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001035	1.00	Polyketide, NRP	pneumocandin B0, pneumocandin A0	Glarea lozoyensis ATCC 20868
BGC0002525	0.70	Polyketide	fusarubin, 1233A, 1233B, NG-391, lucilactaene	Fusarium sp.
BGC0000146	0.67	Polyketide	solanapyrone D	Alternaria solani
BGC0002191	0.66	Polyketide	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0002194	0.65	Polyketide	epipyrone A	Epicoccum nigrum
BGC0002515	0.65	Polyketide	solanapyrone A	Ascochyta rabiei
BGC0002017	0.65	NRP	potensibactin	Nocardiopsis potens DSM 45234
BGC0002518	0.65	NRP	syringafactin A, syringafactin C	Pseudomonas sp. SZ57
BGC0001899	0.64	Polyketide	abscisic acid	Leptosphaeria maculans JN3
BGC0002135	0.64	NRP	bovienimide A	Xenorhabdus bovienii SS-2004

Similar known gene clusters from MIBiG 4.0

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 20469 - 103446:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-pk) + (X - Thr - X - X - X - X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 20469 - 68499:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-pk) + (X - Thr - X - X - X - X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 30153 - 91843:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-pk) + (X - Thr - X - X - X - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

GLAREA_10034

PKS_AT (602..906): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; Propionyl-CoA: 62.5%; 2-Methylbutyryl-CoA: 62.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 83.2
Methoxymalonyl-CoA: 77.0
Malonyl-CoA: 56.6
Ethylmalonyl-CoA: 46.5
Propionyl-CoA: 46.1
Benzoyl-CoA: 37.2
Isobutyryl-CoA: 37.0
2-Methylbutyryl-CoA: 36.3
trans-1,2-CPDA: 26.4
3-Methylbutyryl-CoA: 26.4
fatty_acid: 25.9
CHC-CoA: 25.5
inactive: 7.4
Acetyl-CoA: 6.0

GLAREA_10035

Search NORINE for peptide: strict or relaxed

AMP-binding (674..1052): X

nrpys: dhOrn

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVCEASSIVK
Nearest Stachelhaus code(s):: dhOrn DVCEASSIVK (100% 8Å match); Orn DVCEASSIVK (88% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1757..2154): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAQTAVAIHK
Nearest Stachelhaus code(s):: Thr DAQTAVAIHK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2848..3245): X

nrpys: 4OH-Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVSSATTVCK
Nearest Stachelhaus code(s):: 4OH-Pro DVSSATTVCK (100% 8Å match); Pro DVSSATTVCK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (3919..4320): X

nrpys: dohHty

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DGEAVGCIFK
Nearest Stachelhaus code(s):: dohHty DGEAVGCIFK (100% 8Å match); ohHty DGEAVGCIFK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (5012..5414): X

nrpys: bOH-Gln

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAQNIASINK
Nearest Stachelhaus code(s):: bOH-Gln DAQNIASINK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (6110..6507): X

nrpys: 3OH-Pro, 3S-oh-4S-mePro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DNTMITAMSK
Nearest Stachelhaus code(s):: 3OH-Pro or 3S-oh-4S-mePro DNTMITAMSK (100% 8Å match); 3S-oh-4S-mePro DNTMITAMSK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

GLAREA_10043

AMP-binding (39..463): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: GLQYIGGVCK
Nearest Stachelhaus code(s):: Lin AYVYIGGVFK (47% 8Å match); Leu or Phe DAWTIGGVCK (29% 8Å match)
Stachelhaus code match:: 60% (weak)