BGC0001036 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001036 (Streptomyces sp. MK498-98F14)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	NRPS,T1PKS	1	73,445	High	polyoxypeptin	NRPS:Type I+PKS

BGC0001036 - Region 1 - NRPS,T1PKS

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Location: 1 - 73,445 nt. (total: 73,445 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 73,446
Label		BGC0001036

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

AGZ15446.1: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

AGZ15447.1: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

AGZ15465.1: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

AGZ15470.1: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

Transferases: acylase acyltransferase aminotransferase halogenase

AGZ15457.1: halogenase

smcogs: SMCOG1119: halogenase

AGZ15458.1: polyketide synthase associated protein papA3

smcogs: SMCOG1209: polyketide synthase associated protein papA3

AGZ15466.1: aminotransferase

smcogs: SMCOG1019: aminotransferase

AGZ15472.1: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

AGZ15473.1: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

AGZ15475.1: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

Hydrolases: esterase

AGZ15461.1: thioesterase

smcogs: SMCOG1004: thioesterase

AGZ15471.1: thioesterase

smcogs: SMCOG1004: thioesterase

AGZ15477.1: thioesterase

smcogs: SMCOG1004: thioesterase

Ligases: ligase synthase

AGZ15443.1: 3-dehydroquinate synthase

smcogs: SMCOG1183: 3-dehydroquinate synthase

AGZ15448.1: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs: SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

AGZ15451.1: 1-deoxy-D-xylulose-5-phosphate synthase

smcogs: SMCOG1110: 1-deoxy-D-xylulose-5-phosphate synthase

AGZ15455.1: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

AGZ15459.1: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

AGZ15460.1: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

AGZ15474.1: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

AGZ15476.1: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001036.5	1.63	NRPS, PKS	polyoxypeptin	Streptomyces sp. MK498-98F14
BGC0002135.2	1.07	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001833.4	1.07	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001825.4	1.06	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001128.4	1.03	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	1.03	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001284.4	0.99	PKS	alternariol	Parastagonospora nodorum SN15
BGC0002154.2	0.99	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001166.5	0.99	NRPS	HC-toxin	Alternaria jesenskae
BGC0001258.3	0.98	PKS	1,3,6,8-tetrahydroxynaphthalene	Glarea lozoyensis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001036.5	0.99	NRPS, PKS	polyoxypeptin	Streptomyces sp. MK498-98F14
BGC0001276.3	0.76	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0002135.2	0.75	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001833.4	0.73	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0000041.3	0.72	PKS	oronofacic acid	Pseudomonas syringae
BGC0001128.4	0.72	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001135.5	0.71	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002286.3	0.71	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.71	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001825.4	0.71	NRPS	xenematide	Xenorhabdus nematophila AN6/1

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 73445:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (D-X - Ala) + (D-X - X) + (NMe-Val) + (pk) + (ccemal) + (redmal) + (Me-mal) + (X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 0 - 54220:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (D-X - Ala) + (D-X - X) + (NMe-Val) + (pk) + (ccemal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 29279 - 73445:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X) + (Me-mal) + (redmal) + (ccemal) + (pk) + (NMe-Val)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 4, location 47951 - 73445:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk) + (ccemal) + (redmal) + (Me-mal) + (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

AGZ15455.1: X

AMP-binding (19..425): X

nrpys: Leu, Ala

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DLRHLGQDVK
Nearest Stachelhaus code(s):: Leu or Ala DLRHLGQDVK (100% 8Å match); Leu or Ser DLRHLGQDVK (88% 8Å match)
Stachelhaus code match:: 100% (strong)

AGZ15458.1: X - Ala

Search NORINE for peptide: strict or relaxed

AMP-binding (465..862): X

nrpys: Piz

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVFSVASYAK
Nearest Stachelhaus code(s):: Piz DVFSVASYAK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2012..2391): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: DVPDEALVEK
Nearest Stachelhaus code(s):: Ala DVPDEALVEK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AGZ15459.1: X - X

AMP-binding (682..1075): X

nrpys: Piz

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: ala (Ala)

Stachelhaus prediction details:

Stachelhaus sequence:: DVFSIAAYAK
Nearest Stachelhaus code(s):: Piz DVFSIAAYAK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2224..2615): X

nrpys: 3R-oh-3-mePro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: ala (Ala)

Stachelhaus prediction details:

Stachelhaus sequence:: DVFCNSSYAK
Nearest Stachelhaus code(s):: 3R-oh-3-mePro DVFCNSSYAK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AGZ15460.1: Val

Search NORINE for peptide: strict or relaxed

AMP-binding (466..845): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: DAPFEALVEK
Nearest Stachelhaus code(s):: Val DAPFEALVEK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AGZ15472.1: pk

PKS_AT (537..834): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 201.5
Methylmalonyl-CoA: 194.5
Methoxymalonyl-CoA: 160.6
2-Methylbutyryl-CoA: 117.1
Propionyl-CoA: 107.6
trans-1,2-CPDA: 106.9
CHC-CoA: 102.2
Malonyl-CoA: 101.0
Benzoyl-CoA: 60.3
Isobutyryl-CoA: 59.7
Acetyl-CoA: 40.4
3-Methylbutyryl-CoA: 31.6
inactive: 30.8
fatty_acid: 21.9

AGZ15473.1: emal

PKS_AT (584..882): emal

ATSignature: Ethylmalonyl-CoA, Methoxymalonyl-CoA

Top 3 matches:: Ethylmalonyl-CoA: 87.5%; Methoxymalonyl-CoA: 87.5%; Methylmalonyl-CoA: 79.2%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 197.6
Methylmalonyl-CoA: 188.6
Methoxymalonyl-CoA: 160.6
2-Methylbutyryl-CoA: 138.9
Malonyl-CoA: 120.0
Propionyl-CoA: 116.0
trans-1,2-CPDA: 107.1
CHC-CoA: 96.2
Isobutyryl-CoA: 72.8
Benzoyl-CoA: 45.3
inactive: 44.5
Acetyl-CoA: 40.9
fatty_acid: 31.9
3-Methylbutyryl-CoA: 30.2

AGZ15474.1: mal

PKS_AT (571..869): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; CE-Malonyl-CoA: 62.5%; Methylmalonyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 154.9
Methylmalonyl-CoA: 69.2
Methoxymalonyl-CoA: 63.4
inactive: 58.5
Ethylmalonyl-CoA: 39.1
Propionyl-CoA: 36.1
CHC-CoA: 31.7
2-Methylbutyryl-CoA: 27.6
Isobutyryl-CoA: 24.6
trans-1,2-CPDA: 21.3
Acetyl-CoA: 16.9
Benzoyl-CoA: 16.7
fatty_acid: 14.7
3-Methylbutyryl-CoA: 0.0

AGZ15475.1: mmal

PKS_AT (577..873): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 219.1
Ethylmalonyl-CoA: 214.0
Methoxymalonyl-CoA: 182.4
2-Methylbutyryl-CoA: 167.6
trans-1,2-CPDA: 136.9
Propionyl-CoA: 127.6
Malonyl-CoA: 113.0
CHC-CoA: 104.9
Isobutyryl-CoA: 95.0
Benzoyl-CoA: 75.6
3-Methylbutyryl-CoA: 44.8
Acetyl-CoA: 43.0
fatty_acid: 39.4
inactive: 36.1

AGZ15476.1: X

AMP-binding (480..882): X

nrpys: 3OH-Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: DTLWWGGGFK
Nearest Stachelhaus code(s):: 3OH-Leu DTLWWGGGFK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

Link to PARAS substrate predictor