BGC0001045 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001045 (Pseudomonas sp. Q71576)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	NRPS	1	54,335	spiruchostatin A	NRPS:Type I+PKS:Type I	100%

BGC0001045 - Region 1 - NRPS

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Location: 1 - 54,335 nt. (total: 54,335 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 54,336
Label		BGC0001045

Detailed domain annotation

Selected features only

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Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001045	1.00	NRP, Polyketide	spiruchostatin A	Pseudomonas sp. Q71576
BGC0000964	0.38	NRP, Polyketide	burkholdac A, burkholdac B	Burkholderia thailandensis E264
BGC0000016	0.35	Other	amphotericin B	Streptomyces nodosus
BGC0000343	0.32	NRP	enterobactin	Pseudomonas sp. J465
BGC0000993	0.30	NRP, Polyketide	FR901228	Chromobacterium violaceum
BGC0001671	0.29	NRP	monobactam	Agrobacterium tumefaciens
BGC0002287	0.29	NRP	mutanocyclin, leuvalin, tyrvalin	Streptococcus mutans
BGC0002058	0.29	Polyketide, NRP	mutanocyclin	Streptococcus mutans B04Sm5
BGC0001502	0.29	NRP	amonabactin P 750	Aeromonas hydrophila subsp. hydrophila ATCC 7966
BGC0002437	0.29	NRP	thermoactinoamide A	Thermoactinomyces sp. AS95

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001045	1.00	NRP, Polyketide	spiruchostatin A	Pseudomonas sp. Q71576
BGC0000964	0.70	NRP, Polyketide	burkholdac A, burkholdac B	Burkholderia thailandensis E264
BGC0000016	0.68	Other	amphotericin B	Streptomyces nodosus
BGC0000343	0.65	NRP	enterobactin	Pseudomonas sp. J465
BGC0000993	0.64	NRP, Polyketide	FR901228	Chromobacterium violaceum
BGC0001671	0.63	NRP	monobactam	Agrobacterium tumefaciens
BGC0002287	0.63	NRP	mutanocyclin, leuvalin, tyrvalin	Streptococcus mutans
BGC0002058	0.63	Polyketide, NRP	mutanocyclin	Streptococcus mutans B04Sm5
BGC0001502	0.62	NRP	amonabactin P 750	Aeromonas hydrophila subsp. hydrophila ATCC 7966
BGC0002437	0.62	NRP	thermoactinoamide A	Thermoactinomyces sp. AS95

Similar known gene clusters from MIBiG 4.0

Module view

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 52109:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Cys) + (D-Ala - D-Cys - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

spiA

Search NORINE for peptide: strict or relaxed

CAL_domain (152..469): pk

minowa: NH2

Prediction, score:

NH2: 130.8
Acetyl-CoA: 48.6
fatty_acid: 16.5
AHBA: 15.6
shikimic_acid: 11.3

AMP-binding (1088..1471): Cys

nrpys: Cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: DLYNLSLIWK
Nearest Stachelhaus code(s):: Cys DLYNLSLIWK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

spiDE1

Search NORINE for peptide: strict or relaxed

AMP-binding (525..919): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: DTFWLGGTFK
Nearest Stachelhaus code(s):: Ala DTFWLGGTFK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2021..2412): Cys

nrpys: Cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: DLFEMSLIWK
Nearest Stachelhaus code(s):: Cys DLFEMSLIWK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (3489..3876): X

nrpys: Ile, Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DFLCLGVVIK
Nearest Stachelhaus code(s):: Ile or Val DFLCLGVVIK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

spiP

PKS_AT (5..288): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; inactive: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 87.2
Methoxymalonyl-CoA: 41.0
inactive: 32.8
fatty_acid: 27.5
Methylmalonyl-CoA: 25.5
Propionyl-CoA: 21.9
Acetyl-CoA: 12.6
Isobutyryl-CoA: 10.2
2-Methylbutyryl-CoA: 6.4
trans-1,2-CPDA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0