Genomic loci for this biosynthetic pathway

Cluster Type From To
The following clusters are from record BGC0001046.1:
Cluster 1NRP / Polyketide / Saccharide180894

BGC0001046, streptolydigin biosynthetic gene cluster from Streptomyces lydicus. Locus 1. Full MIBiG entry.

Chemical compounds

Compound: Streptolydigin
SMILES string: Copy to clipboard
Molecular formula: C32H44N2O9
Exact molecular mass: 601.7 Da ([M+H]+)
Molecular activity: Antibacterial
Molecular target: RNA polymerase

Class-specific details

Biosynthetic class(es):
NRP / Polyketide / Saccharide
Polyketide subclass:
Other (cyclic)
Polyketide synthase subclass:
Modular type I
Starter unit:
Acetyl-CoA
Thioesterase type:
Type II
Release / cyclization type:
Other

Modular polyketide synthases:
slgA1 (CBA11584.1)
Module 0
AT specificity: Malonyl-CoA
Evidence for specificity: Unknown
Module 1
AT specificity: Methylmalonyl-CoA
Evidence for specificity: Unknown
Module 2
AT specificity: Malonyl-CoA
Evidence for specificity: Unknown
Module 3
AT specificity: Methylmalonyl-CoA
Evidence for specificity: Unknown

slgA2 (CBA11583.1)
Module 4
AT specificity: Methylmalonyl-CoA
Evidence for specificity: Unknown
Module 5
AT specificity: Methylmalonyl-CoA
Evidence for specificity: Unknown

slgA3 (CBA11582.1)
Module 6
AT specificity: Malonyl-CoA
Evidence for specificity: Unknown
Module 7
AT specificity: Malonyl-CoA
Evidence for specificity: Unknown

Gene cluster description

streptolydigin (BGC0001046). Gene Cluster 1. Biosynthetic class = NRP/Polyketide/Saccharide. GenBank FN433113. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Domain annotation

Homologous known gene clusters

General MIBiG information on this cluster

Complete gene cluster sequence?complete
Evidence for cluster-compound connection:Knock-out studies, Sequence-based prediction
MIxS-compliance:Unknown
Contact for this cluster:Jose A. Salas (University of Oviedo)

Literature references

1. Olano C et al. (2009) Deciphering biosynthesis of the RNA polymerase inhibitor streptolydigin and generation of glycosylated derivatives. Chem Biol 16(10):1031-44. doi: 10.1016/j.chembiol.2009.09.015.
2. Horna DH et al. (2011) Biosynthesis of the RNA polymerase inhibitor streptolydigin in Streptomyces lydicus: tailoring modification of 3-methyl-aspartate. J Bacteriol 193(10):2647-51. doi: 10.1128/JB.00108-11. Epub 2011 Mar
3. Gomez C et al. (2011) Amino acid precursor supply in the biosynthesis of the RNA polymerase inhibitor streptolydigin by Streptomyces lydicus. J Bacteriol 193(16):4214-23. doi: 10.1128/JB.05062-11. Epub 2011 Jun
4. Gomez C et al. (2012) Novel compounds produced by Streptomyces lydicus NRRL 2433 engineered mutants altered in the biosynthesis of streptolydigin. J Antibiot (Tokyo) 65(7):341-8. doi: 10.1038/ja.2012.37. Epub 2012 May
5. Gomez C et al. (2012) Participation of putative glycoside hydrolases SlgC1 and SlgC2 in the biosynthesis of streptolydigin in Streptomyces lydicus. Microb Biotechnol 5(5):663-7. doi: 10.1111/j.1751-7915.2012.00352.x.
6. Gomez C et al. (2012) Three pathway-specific regulators control streptolydigin biosynthesis in Streptomyces lydicus. Microbiology 158(Pt 10):2504-14. doi: 10.1099/mic.0.061325-0. Epub 2012