Genomic loci for this biosynthetic pathway

Cluster Type From To
The following clusters are from record BGC0001048.1:
Cluster 1NRP / Polyketide / Saccharide180158

BGC0001048, tallysomycin biosynthetic gene cluster from Streptoalloteichus hindustanus. Locus 1. Full MIBiG entry.

Chemical compounds

Compound: tallysomycin A
PubChem ID: 430602
SMILES string: Copy to clipboard
Molecular formula: C68H110N22O27S2
Average molecular mass: 1731.875 Da
Molecular activity: Cytotoxic
Molecular target: DNA and RNA

Class-specific details

Biosynthetic class(es):
NRP / Polyketide / Saccharide
Glycopeptide (linear)
Thioesterase type:
Unknown
Release / cyclization type:
Unknown

Nonribosomal peptide synthetases:
tlmX (ABL74936.1)
Module 4
A specificity: Asparigine
Evidence for specificity: Sequence-based prediction
C domain subtype: LCL
Module 5
A specificity: Histidine
Evidence for specificity: Sequence-based prediction
C domain subtype: DCL
tlmIX (ABL74937.1)
Module 6
A specificity: Alanine
Evidence for specificity: Sequence-based prediction
C domain subtype: LCL
tlmVII (ABL74939.1)
Module 8
A specificity: Threonine
Evidence for specificity: Sequence-based prediction
C domain subtype: LCL
tlmVI (ABL74940.1)
Module 1
A specificity: Unknown
Evidence for specificity: Unknown
C domain subtype: Starter
Module 2
A specificity: Serine
Evidence for specificity: Sequence-based prediction
C domain subtype: LCL
Module 3
A specificity: Asparigine
Evidence for specificity: Sequence-based prediction
C domain subtype: DCL
tlmV (ABL74941.1)
Module 3
A specificity: Unknown
Evidence for specificity: Unknown
C domain subtype: DCL
tlmIV (ABL74945.1)
Module 10
A specificity: Cysteine
Evidence for specificity: Sequence-based prediction
C domain subtype: Heterocyclization
Scaffold-modifying domain: Other
Module 11
A specificity: Unknown
Evidence for specificity: Unknown
C domain subtype: Heterocyclization
Module 9
A specificity: Beta-alanine
Evidence for specificity: Sequence-based prediction
C domain subtype: LCL
tlmIII (ABL74946.1)
Module 11
A specificity: Unknown
Evidence for specificity: Unknown
C domain subtype: N/A
Scaffold-modifying domain: Oxidation
Polyketide subclass:
Other (linear)
Polyketide synthase subclass:
Modular type I
Starter unit:
Other
Thioesterase type:
None
Release / cyclization type:
None

Modular polyketide synthases:
tlmVIII (ABL74938.1)
Module 7
AT specificity: Malonyl-CoA
Evidence for specificity: Sequence-based prediction
KR stereochemistry: B-group
Scaffold-modifying domain: None

Gene cluster description

tallysomycin (BGC0001048). Gene Cluster 1. Biosynthetic class = NRP/Polyketide/Saccharide. GenBank EF032505, positions 1198-80111. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Domain annotation

Homologous known gene clusters

General MIBiG information on this cluster

Complete gene cluster sequence?complete
Evidence for cluster-compound connection:Knock-out studies
MIxS-compliance:Unknown
Contact for this cluster:Xiaohui Yan (The Scripps Research Institute)

Literature references

1. Tao M et al. (2007) The tallysomycin biosynthetic gene cluster from Streptoalloteichus hindustanus E465-94 ATCC 31158 unveiling new insights into the biosynthesis of the bleomycin family of antitumor antibiotics. Mol Biosyst 3(1):60-74. doi: 10.1039/b615284h. Epub 2006 Nov 28.
2. Wang L et al. (2009) Functional characterization of tlmK unveiling unstable carbinolamide intermediates in the tallysomycin biosynthetic pathway. J Biol Chem 284(13):8256-64. doi: 10.1074/jbc.M900640200. Epub 2009
3. Coughlin JM et al. (2014) BlmB and TlmB provide resistance to the bleomycin family of antitumor antibiotics by N-acetylating metal-free bleomycin, tallysomycin, phleomycin, and zorbamycin. Biochemistry 53(44):6901-9. doi: 10.1021/bi501121e. Epub 2014 Oct
4. Galm U et al. (2011) Comparative analysis of the biosynthetic gene clusters and pathways for three structurally related antitumor antibiotics: bleomycin, tallysomycin, and zorbamycin. J Nat Prod 74(3):526-36. doi: 10.1021/np1008152. Epub 2011 Jan 6.
5. Tao M et al. (2010) Functional characterization of tlmH in Streptoalloteichus hindustanus E465-94 ATCC 31158 unveiling new insight into tallysomycin biosynthesis and affording a novel bleomycin analog. Mol Biosyst 6(2):349-56. doi: 10.1039/b918106g. Epub 2009 Dec 1.
6. Zhang N et al. (2010) Improved production of the tallysomycin H-1 in Streptoalloteichus hindustanus SB8005 strain by fermentation optimization. Appl Microbiol Biotechnol 86(5):1345-53. doi: