BGC0001051: thuggacin A biosynthetic gene cluster from Sorangium cellulosum
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Location: 17,491 - 76,320 nt. (total: 58,830 nt).
This entry is originally from NCBI GenBank GQ981380.1.

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
TTA codons
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Gene details
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General
Compounds
Genes
Polyketide
NRP
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0001051
Short description thuggacin A biosynthetic gene cluster from Sorangium cellulosum
Status Minimal annotation: no
A minimal annotation only contains information on the BGC loci and one or more linked chemical product(s)

Completeness: complete
Whether the loci encodes everything needed for the pathway producing the compound(s)
Biosynthetic class(es)
  • NRP
  • Polyketide (Other)
Loci NCBI GenBank: GQ981380.1
Compounds
  • thuggacin A
Species Sorangium cellulosum [taxonomy]
References
Chemical products information
thuggacin A
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C35H53N1O7S1
Chemical database entries
NPAtlas
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • ADH04638.1
  • tgaE
 17492 - 18565 (-) monooxygenase
  • Tailoring (Oxidation)
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  • ADH04639.1
  • tgaA
 18917 - 44572 (+) TgaA
  • Scaffold biosynthesis
Sequence-based prediction
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  • ADH04640.1
  • tgaB
 44613 - 54896 (+) TgaB
  • Scaffold biosynthesis
Sequence-based prediction
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  • ADH04641.1
  • tgaC
 55016 - 75007 (+) TgaC
  • Scaffold biosynthesis
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  • ADH04642.1
  • tgaD
 75157 - 76320 (+) alcohol dehydrogenase
  • Tailoring (Oxidation)
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Polyketide-specific information
Subclass Other
Starter unit Acetyl-CoA
Cyclic? yes
Release type
  • Macrolactonization
Polyketide-synthases
Genes Properties Modules
tgaA
+
tgaC
+
tgaB
+
tgaC 		Synthase subclass: Modular type I
Thioesterases:
  • tgaC (Type I)
Module 1
Specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
Genes: tgaA
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Dehydratase, Enoylreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
Module 2
Specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
Genes: tgaA
Core domains: Ketosynthase, Acyltransferase, Dehydratase, Thiolation (ACP/PCP)
Module 3
Specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
Genes: tgaA
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Dehydratase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
Module 4
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: tgaA
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
Module 5
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: tgaA
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
Module 6
Specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
Genes: tgaB
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: D-OH
Module 7
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: tgaB
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
Module 8
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: tgaC
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: D-OH
Module 9
Specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
Genes: tgaC
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: D-OH
Module 11
Genes: tgaC
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
NRP-specific information
Subclass N/A
Cyclic? no
Release type
  • Macrolactonization
NRP-synthases
Gene Modules
tgaC
Module 10
Specificity: cysteine
Evidence for specificity:
  • Sequence-based prediction
Condensation domain type: Heterocyclization
Modification domains: Oxidation
Annotation changelog
MIBiG version Submitter Notes
1.0
  • Hidden contributor (ID: 7SR74ARK6JXRBGJRGO2LUHE7, no GDPR consent given).
  • Submitted
2.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Migrated from v1.4
  • Updated compound(s) information (NPAtlas curation)
3.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Corrected NRP module activity
  • Remove leading/trailing whitespace in gene identifiers
  • Removed ketoreductase stereochemistry annotation from modules without ketoreductases
  • Refined reference coordinates
  • Changed amino acid substrates to lower case
3.1
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Update chemical activity to schema version 2.11
Detailed domain annotation
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