BGC0001052 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001052 (Streptomyces sp. 307-9)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS,NRPS	1	55,522	tirandamycin	NRPS:Type I+PKS:Type I	100%

BGC0001052 - Region 1 - NRPS,T1PKS

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Location: 1 - 55,522 nt. (total: 55,522 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: cds(Condensation and (AMP-binding or A-OX))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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resistance

TTA codons

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 55,523
Label		BGC0001052

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001052	1.51	NRP, Polyketide	tirandamycin	Streptomyces sp. 307-9
BGC0000016	0.78	Other	amphotericin B	Streptomyces nodosus
BGC0002631	0.64	RiPP	myxarylin	Pyxidicoccus fallax
BGC0000844	0.61	Other	clavulanic acid	Streptomyces clavuligerus
BGC0002171	0.59	NRP, Polyketide	pyrophen, campyrone B	Aspergillus niger ATCC 1015
BGC0002275	0.59	NRP, Polyketide	pyrophen, campyrone B	Aspergillus niger CBS 101883
BGC0001276	0.58	Polyketide	6-methylsalicyclic acid	Aspergillus terreus
BGC0002226	0.57	NRP	acu-dioxomorpholine B, acu-dioxomorpholine A	Aspergillus aculeatus ATCC 16872
BGC0000160	0.56	Polyketide	terreic acid	Aspergillus terreus NIH2624
BGC0001050	0.54	NRP, Polyketide	thalassospiramide A	Tistrella bauzanensis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001052	1.00	NRP, Polyketide	tirandamycin	Streptomyces sp. 307-9
BGC0000016	0.71	Other	amphotericin B	Streptomyces nodosus
BGC0002631	0.65	RiPP	myxarylin	Pyxidicoccus fallax
BGC0000996	0.64	NRP, Polyketide	frontalamide B, frontalamide A	Streptomyces sp. SPB78
BGC0000844	0.64	Other	clavulanic acid	Streptomyces clavuligerus
BGC0001068	0.63	Terpene, Polyketide	pyripyropene A	unidentified unclassified sequences.
BGC0002171	0.63	NRP, Polyketide	pyrophen, campyrone B	Aspergillus niger ATCC 1015
BGC0002275	0.63	NRP, Polyketide	pyrophen, campyrone B	Aspergillus niger CBS 101883
BGC0001276	0.63	Polyketide	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273	0.63	Polyketide	asperlactone	Aspergillus ochraceus

Similar known gene clusters from MIBiG 4.0

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 55522:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal - Me-ohmal - ohmal - Me-ccmal) + (Me-ohmal - Me-ccmal) + (ccmal - mal) + (Gly)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 20522 - 55522:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-ohmal - Me-ccmal) + (ccmal - mal) + (Gly)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ADC79637.1

PKS_AT (544..831): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 189.0
inactive: 78.3
Methoxymalonyl-CoA: 48.9
Methylmalonyl-CoA: 38.0
Propionyl-CoA: 22.8
Isobutyryl-CoA: 16.0
Acetyl-CoA: 15.0
2-Methylbutyryl-CoA: 12.7
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (1553..1840): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 87.5%; Ethylmalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 204.4
Ethylmalonyl-CoA: 192.0
Methoxymalonyl-CoA: 170.8
2-Methylbutyryl-CoA: 130.1
trans-1,2-CPDA: 123.6
Propionyl-CoA: 112.3
Malonyl-CoA: 90.6
CHC-CoA: 85.0
Isobutyryl-CoA: 61.3
fatty_acid: 56.9
Acetyl-CoA: 49.2
Benzoyl-CoA: 48.5
3-Methylbutyryl-CoA: 37.4
inactive: 25.6

PKS_AT (3066..3354): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 185.6
inactive: 72.4
Methoxymalonyl-CoA: 43.7
Methylmalonyl-CoA: 35.5
Isobutyryl-CoA: 18.9
Acetyl-CoA: 18.9
Propionyl-CoA: 17.3
2-Methylbutyryl-CoA: 7.2
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (4554..4842): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 216.4
Ethylmalonyl-CoA: 208.5
Methoxymalonyl-CoA: 183.1
2-Methylbutyryl-CoA: 140.3
trans-1,2-CPDA: 126.4
Propionyl-CoA: 116.7
Malonyl-CoA: 103.3
CHC-CoA: 97.8
Isobutyryl-CoA: 74.4
Benzoyl-CoA: 59.0
fatty_acid: 56.1
Acetyl-CoA: 55.6
3-Methylbutyryl-CoA: 42.5
inactive: 31.7

ADC79638.1

PKS_AT (569..857): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 216.4
Ethylmalonyl-CoA: 208.5
Methoxymalonyl-CoA: 183.1
2-Methylbutyryl-CoA: 140.3
trans-1,2-CPDA: 126.4
Propionyl-CoA: 116.7
Malonyl-CoA: 103.3
CHC-CoA: 97.8
Isobutyryl-CoA: 74.4
Benzoyl-CoA: 59.0
fatty_acid: 56.1
Acetyl-CoA: 55.6
3-Methylbutyryl-CoA: 42.5
inactive: 31.7

PKS_AT (2119..2407): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 83.3%; Ethylmalonyl-CoA: 79.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 201.7
Ethylmalonyl-CoA: 188.7
Methoxymalonyl-CoA: 169.9
2-Methylbutyryl-CoA: 128.8
trans-1,2-CPDA: 123.0
Propionyl-CoA: 113.1
Malonyl-CoA: 87.7
CHC-CoA: 87.7
Isobutyryl-CoA: 58.1
fatty_acid: 55.3
Benzoyl-CoA: 51.8
Acetyl-CoA: 49.7
3-Methylbutyryl-CoA: 37.9
inactive: 26.0

ADC79639.1

PKS_AT (555..853): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 87.5%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 225.1
inactive: 129.5
Methoxymalonyl-CoA: 73.7
Methylmalonyl-CoA: 62.9
Isobutyryl-CoA: 38.7
Propionyl-CoA: 31.0
Benzoyl-CoA: 25.4
CHC-CoA: 21.5
Ethylmalonyl-CoA: 20.0
3-Methylbutyryl-CoA: 17.8
Acetyl-CoA: 15.5
fatty_acid: 14.8
2-Methylbutyryl-CoA: 12.3
trans-1,2-CPDA: 5.9

PKS_AT (2281..2578): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 75.0%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 206.3
inactive: 104.5
Methoxymalonyl-CoA: 60.2
Methylmalonyl-CoA: 53.1
Propionyl-CoA: 35.1
Isobutyryl-CoA: 25.5
Acetyl-CoA: 22.7
Ethylmalonyl-CoA: 20.1
2-Methylbutyryl-CoA: 17.9
CHC-CoA: 17.7
fatty_acid: 13.6
Benzoyl-CoA: 11.6
trans-1,2-CPDA: 9.5
3-Methylbutyryl-CoA: 0.0

ADC79642.1

Search NORINE for peptide: strict or relaxed

AMP-binding (460..849): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: DILQVGVIWK
Nearest Stachelhaus code(s):: Gly DILQVGVIWK (100% 8Å match)
Stachelhaus code match:: 100% (strong)