BGC0001057 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001057 (Fusarium graminearum (Gibberella zeae))

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	NRPS,T1PKS	1	49,993	High	zearalenone	NRPS:Type I+PKS

BGC0001057 - Region 1 - NRPS,T1PKS

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Location: 1 - 49,993 nt. (total: 49,993 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 49,994
Label		BGC0001057

Tailoring functions

Oxidoreductases: dehydrogenase oxidase oxidoreductase

ALD: aldehyde dehydrogenase

smcogs: SMCOG1017: aldehyde dehydrogenase

KAT: aldo/keto reductase family oxidoreductase

smcogs: SMCOG1039: aldo/keto reductase family oxidoreductase

ZEB1: FAD linked oxidase domain protein

smcogs: SMCOG1138: FAD linked oxidase domain protein

Ligases: ligase synthase

NPS: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

PKS13: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

PKS4: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001057.4	1.85	NRPS, PKS	zearalenone	Fusarium graminearum
BGC0001249.5	0.87	NRPS	dimethylcoprogen	Alternaria alternata
BGC0002164.2	0.76	NRPS	peramine	Epichloe festucae
BGC0001261.3	0.69	NRPS	AM-toxin	Alternaria alternata
BGC0002192.3	0.67	PKS	FR901512	fungal sp. No.14919
BGC0002175.3	0.66	PKS	YWA1	Aspergillus oryzae RIB40
BGC0000107.3	0.66	PKS	naphthopyrone	Aspergillus nidulans FGSC A4
BGC0002154.2	0.66	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001257.3	0.66	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001057.4	0.99	NRPS, PKS	zearalenone	Fusarium graminearum
BGC0001249.5	0.74	NRPS	dimethylcoprogen	Alternaria alternata
BGC0002164.2	0.70	NRPS	peramine	Epichloe festucae
BGC0001261.3	0.68	NRPS	AM-toxin	Alternaria alternata
BGC0002192.3	0.66	PKS	FR901512	fungal sp. No.14919
BGC0002175.3	0.66	PKS	YWA1	Aspergillus oryzae RIB40
BGC0000107.3	0.66	PKS	naphthopyrone	Aspergillus nidulans FGSC A4
BGC0002154.2	0.66	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001257.3	0.66	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 49993:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk) + (mal) + (X - X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 0 - 36031:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk) + (mal) + (X - X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 205 - 49993:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk) + (mal) + (X - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

NPS: X - X

AMP-binding (221..620): X

nrpys: Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVSNLSTFKK
Nearest Stachelhaus code(s):: Orn DVSELGTINK (44% 8Å match)
Stachelhaus code match:: 60% (weak)

AMP-binding (1301..1695): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVLCVAWLSK
Nearest Stachelhaus code(s):: Pro DVLLVAGVLK (53% 8Å match)
Stachelhaus code match:: 60% (weak)

PKS13: mal

PKS_AT (887..1182): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 62.5%; Methylmalonyl-CoA: 58.3%; inactive: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 109.8
Methoxymalonyl-CoA: 69.9
Methylmalonyl-CoA: 65.2
inactive: 58.0
Isobutyryl-CoA: 44.8
Propionyl-CoA: 36.3
Benzoyl-CoA: 34.8
Ethylmalonyl-CoA: 32.2
2-Methylbutyryl-CoA: 30.3
Acetyl-CoA: 29.1
CHC-CoA: 23.7
fatty_acid: 20.1
3-Methylbutyryl-CoA: 19.9
trans-1,2-CPDA: 19.1

PKS4: pk

PKS_AT (537..838): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; Methylmalonyl-CoA: 66.7%; Ethylmalonyl-CoA: 62.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 92.7
Methoxymalonyl-CoA: 81.6
Malonyl-CoA: 72.7
Ethylmalonyl-CoA: 57.3
Isobutyryl-CoA: 53.6
Propionyl-CoA: 38.5
Benzoyl-CoA: 35.8
CHC-CoA: 25.8
trans-1,2-CPDA: 22.9
fatty_acid: 22.3
Acetyl-CoA: 21.1
2-Methylbutyryl-CoA: 19.1
3-Methylbutyryl-CoA: 12.0
inactive: 11.3

Link to PARAS substrate predictor