BGC0001058 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001058 (Streptomyces pilosus)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	NRPS,NRPS-like	1	84,773	High	zorbamycin	NRPS:Type I+PKS:Type I+saccharide:hybrid/tailoring

BGC0001058 - Region 1 - NRPS,NRPS-like

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Location: 1 - 84,773 nt. (total: 84,773 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX) and not alpha_am_amid)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 84,774
Label		BGC0001058

Tailoring functions

Oxidoreductases: oxygenase

ACG60747.1: Dioxygenase TauD/TfdA

smcogs: SMCOG1046: Dioxygenase TauD/TfdA

zbmVIIc: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

Hydrolases: esterase hydrolase

ACG60736.1: thioesterase

smcogs: SMCOG1004: thioesterase

ACG60746.1: asparagine synthase (glutamine-hydrolyzing)

smcogs: SMCOG1177: asparagine synthase (glutamine-hydrolyzing)

zbmVIId: thioesterase

smcogs: SMCOG1004: thioesterase

Lyases: dehydratase

zbmG: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

zbmL: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Ligases: ligase synthase

ACG60752.1: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

ACG60753.1: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

ACG60755.1: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

ACG60761.1: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

zbmII: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

zbmIII: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

zbmIV: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

zbmIX: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

zbmV: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

zbmVI: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

zbmVIII: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

zbmVIIa: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

zbmVIIb: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

zbmX: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

zbmXI: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001058.5	2.06	NRPS, PKS, saccharide	zorbamycin	Streptomyces flavoviridis
BGC0001132.4	1.61	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001261.3	1.58	NRPS	AM-toxin	Alternaria alternata
BGC0001128.4	1.57	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	1.57	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001135.5	1.55	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002135.2	1.51	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001758.4	1.50	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001833.4	1.49	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001641.4	1.44	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001058.5	0.98	NRPS, PKS, saccharide	zorbamycin	Streptomyces flavoviridis
BGC0002135.2	0.77	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.77	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001758.4	0.77	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001128.4	0.76	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.76	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001261.3	0.75	NRPS	AM-toxin	Alternaria alternata
BGC0001132.4	0.75	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001833.4	0.75	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001641.4	0.74	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 84773:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Asn - His) + (X) + (NH2 - Ser - Asn) + (bAla - Cys - bAla) + (Val) + (X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 0 - 72922:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Asn - His) + (X) + (NH2 - Ser - Asn) + (bAla - Cys - bAla) + (Val)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 46457 - 84773:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (bAla - Cys - bAla) + (Val) + (X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 4, location 51376 - 84773:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (bAla - Cys - bAla) + (Val) + (X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 5, location 60242 - 84773:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Val) + (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

zbmX: Asn - His

Search NORINE for peptide: strict or relaxed

AMP-binding (464..856): Asn

nrpys: Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: DFTKVGEVGK
Nearest Stachelhaus code(s):: Asn DFTKVGEVGK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1530..1931): His

nrpys: His

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DSVLTAEVWK
Nearest Stachelhaus code(s):: His DSVLTAEVWK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

zbmIX: X

AMP-binding (480..880): X

nrpys: Hse

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: ala (Ala)

Stachelhaus prediction details:

Stachelhaus sequence:: DVFSNGLTHK
Nearest Stachelhaus code(s):: Hse DVFSNGLTHK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

zbmVI: NH2 - Ser - Asn

Search NORINE for peptide: strict or relaxed

CAL_domain (42..469): NH2

minowa: NH2

Prediction, score:

NH2: 260.9
fatty_acid: 16.8
Acetyl-CoA: 4.6
AHBA: 2.0
shikimic_acid: 0.0

AMP-binding (1151..1555): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: DVWHLSLIDK
Nearest Stachelhaus code(s):: Ser DVWHLSLIDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2198..2588): Asn

nrpys: Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKVGEVGK
Nearest Stachelhaus code(s):: Asn DLTKVGEVGK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

zbmIV: bAla - Cys

Search NORINE for peptide: strict or relaxed

AMP-binding (499..904): bAla

nrpys: bAla

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: VDALVSLADK
Nearest Stachelhaus code(s):: bAla VDALVSLADK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1589..1994): Cys

nrpys: Cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: DLYNLSMIWK
Nearest Stachelhaus code(s):: Cys DLYNLSMIWK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

zbmIII: bAla

Search NORINE for peptide: strict or relaxed

AMP-binding (39..394): bAla

nrpys: bAla

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: ERYSASLIWK
Nearest Stachelhaus code(s):: bAla ERYSASLIWK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

zbmVIIb: Val

Search NORINE for peptide: strict or relaxed

AMP-binding (13..403): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: DAFWLGGTFK
Nearest Stachelhaus code(s):: Val DAFWLGGTFK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

ACG60755.1: X

AMP-binding (13..416): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DTASISYFIK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

ACG60761.1: X

AMP-binding (550..936): X

nrpys: Val, Leu, Ile, Abu, Ival

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: EPLQVGAGWK
Nearest Stachelhaus code(s):: Gly DILQVGVIWK (53% 8Å match)
Stachelhaus code match:: 60% (weak)

Link to PARAS substrate predictor