BGC0001058 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001058 (Streptomyces pilosus)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	NRPS,NRPS-like	1	84,773	zorbamycin	NRPS:Type I+PKS:Type I+saccharide:hybrid/tailoring	100%

BGC0001058 - Region 1 - NRPS,NRPS-like

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Location: 1 - 84,773 nt. (total: 84,773 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 84,774
Label		BGC0001058

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001058	2.20	NRP, Polyketide, Saccharide	zorbamycin	Streptomyces flavoviridis
BGC0000375	1.31	NRP	indigoidine	Streptomyces chromofuscus
BGC0000901	1.30	Other	ferrichrome	Aspergillus niger
BGC0000457	1.27	NRP	vicibactin	Rhizobium etli CFN 42
BGC0000900	1.26	Other	ferrichrome	Aspergillus oryzae
BGC0002710	1.21	NRP	metachelin C, metachelin A, metachelin A-CE, metachelin B, dimerumic acid 11-mannoside, dimerumic acid	Metarhizium robertsii ARSEF 23
BGC0002508	1.21	Alkaloid	8-azaguanine	Streptomyces pathocidini
BGC0002017	1.20	NRP	potensibactin	Nocardiopsis potens DSM 45234
BGC0001132	1.18	NRP	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0000921	1.18	Other (Shikimate-derived), Polyketide, NRP	promysalin	Pseudomonas putida

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001058	1.00	NRP, Polyketide, Saccharide	zorbamycin	Streptomyces flavoviridis
BGC0002017	0.68	NRP	potensibactin	Nocardiopsis potens DSM 45234
BGC0002043	0.68	RiPP	cittilin A, cittilin B	Myxococcus xanthus DK 1622
BGC0002135	0.67	NRP	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0002518	0.67	NRP	syringafactin A, syringafactin C	Pseudomonas sp. SZ57
BGC0001238	0.67	Other	biotin	Aspergillus nidulans
BGC0000375	0.67	NRP	indigoidine	Streptomyces chromofuscus
BGC0001135	0.67	NRP	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001758	0.67	NRP	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0000457	0.67	NRP	vicibactin	Rhizobium etli CFN 42

Similar known gene clusters from MIBiG 4.0

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 84773:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Asn - His) + (X) + (Ser - Asn) + (bAla - Cys) + (bAla) + (Val) + (X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 0 - 72922:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Asn - His) + (X) + (Ser - Asn) + (bAla - Cys) + (bAla) + (Val)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 46457 - 84773:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (bAla - Cys) + (bAla) + (Val) + (X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 4, location 51376 - 84773:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (bAla - Cys) + (bAla) + (Val) + (X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 5, location 60242 - 84773:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Val) + (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

zbmX

Search NORINE for peptide: strict or relaxed

AMP-binding (464..856): Asn

nrpys: Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: DFTKVGEVGK
Nearest Stachelhaus code(s):: Asn DFTKVGEVGK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1530..1931): His

nrpys: His

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DSVLTAEVWK
Nearest Stachelhaus code(s):: His DSVLTAEVWK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

zbmIX

AMP-binding (480..880): X

nrpys: Hse

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: ala (Ala)

Stachelhaus prediction details:

Stachelhaus sequence:: DVFSNGLTHK
Nearest Stachelhaus code(s):: Hse DVFSNGLTHK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

zbmVI

Search NORINE for peptide: strict or relaxed

CAL_domain (42..469): pk

minowa: NH2

Prediction, score:

NH2: 260.9
fatty_acid: 16.8
Acetyl-CoA: 4.6
AHBA: 2.0
shikimic_acid: 0.0

AMP-binding (1151..1555): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: DVWHLSLIDK
Nearest Stachelhaus code(s):: Ser DVWHLSLIDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2198..2588): Asn

nrpys: Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKVGEVGK
Nearest Stachelhaus code(s):: Asn DLTKVGEVGK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

zbmIV

Search NORINE for peptide: strict or relaxed

AMP-binding (499..904): bAla

nrpys: bAla

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: VDALVSLADK
Nearest Stachelhaus code(s):: bAla VDALVSLADK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1589..1994): Cys

nrpys: Cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: DLYNLSMIWK
Nearest Stachelhaus code(s):: Cys DLYNLSMIWK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

zbmIII

Search NORINE for peptide: strict or relaxed

AMP-binding (39..394): bAla

nrpys: bAla

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: ERYSASLIWK
Nearest Stachelhaus code(s):: bAla ERYSASLIWK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

zbmVIIb

Search NORINE for peptide: strict or relaxed

AMP-binding (13..403): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: DAFWLGGTFK
Nearest Stachelhaus code(s):: Val DAFWLGGTFK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

ACG60755.1

AMP-binding (13..416): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DTASISYFIK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

ACG60761.1

AMP-binding (550..936): X

nrpys: Val, Leu, Ile, Abu, Ival

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: EPLQVGAGWK
Nearest Stachelhaus code(s):: Gly DILQVGVIWK (53% 8Å match)
Stachelhaus code match:: 60% (weak)