BGC0001059 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001059 (Bacillus cereus)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	NRPS,T1PKS	1	65,755	zwittermicin A	NRPS:Type I+PKS	100%

BGC0001059 - Region 1 - NRPS,T1PKS

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Location: 1 - 65,755 nt. (total: 65,755 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 65,756
Label		BGC0001059

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001059	1.91	NRP, Polyketide	zwittermicin A	Bacillus cereus
BGC0002071	0.78	NRP	virginiafactin A, virginiafactin B, virginiafactin C, virginiafactin D	Pseudomonas sp. QS1027
BGC0000979	0.71	NRP, Polyketide	cylindrospermopsin	Aphanizomenon sp. 10E9
BGC0000980	0.71	NRP, Polyketide	cylindrospermopsin	Aphanizomenon sp. 22D11
BGC0000426	0.71	NRP	sevadicin	Paenibacillus larvae
BGC0001890	0.70	NRP	amphi-enterobactin 1, amphi-enterobactin 2, amphi-enterobactin 3, amphi-enterobactin 4	Vibrio campbellii ATCC BAA-1116
BGC0002437	0.70	NRP	thermoactinoamide A	Thermoactinomyces sp. AS95
BGC0001185	0.69	NRP	bacillibactin	Bacillus velezensis FZB42
BGC0001132	0.68	NRP	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001133	0.67	NRP	taxlllaid A	Xenorhabdus bovienii SS-2004

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001059	1.00	NRP, Polyketide	zwittermicin A	Bacillus cereus
BGC0002071	0.71	NRP	virginiafactin A, virginiafactin B, virginiafactin C, virginiafactin D	Pseudomonas sp. QS1027
BGC0000979	0.68	NRP, Polyketide	cylindrospermopsin	Aphanizomenon sp. 10E9
BGC0000980	0.68	NRP, Polyketide	cylindrospermopsin	Aphanizomenon sp. 22D11
BGC0000426	0.68	NRP	sevadicin	Paenibacillus larvae
BGC0001890	0.68	NRP	amphi-enterobactin 1, amphi-enterobactin 2, amphi-enterobactin 3, amphi-enterobactin 4	Vibrio campbellii ATCC BAA-1116
BGC0002437	0.68	NRP	thermoactinoamide A	Thermoactinomyces sp. AS95
BGC0001185	0.67	NRP	bacillibactin	Bacillus velezensis FZB42
BGC0001132	0.67	NRP	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001133	0.67	NRP	taxlllaid A	Xenorhabdus bovienii SS-2004

Similar known gene clusters from MIBiG 4.0

Module view

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 65755:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (ohmal) + (bOH-Tyr - Pro) + (X - ohmal) + (D-Asn) + (X - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

zmaA

PKS_AT (1897..2192): mal

ATSignature: 2-Rhyd-Malonyl-CoA

Top 3 matches:: 2-Rhyd-Malonyl-CoA: 100.0%; Malonyl-CoA: 62.5%; inactive: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 114.1
inactive: 74.2
Methoxymalonyl-CoA: 51.0
Methylmalonyl-CoA: 42.9
Propionyl-CoA: 31.2
Ethylmalonyl-CoA: 14.5
2-Methylbutyryl-CoA: 13.5
CHC-CoA: 13.0
Isobutyryl-CoA: 9.5
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

zmaB

Search NORINE for peptide: strict or relaxed

AMP-binding (274..685): bOH-Tyr

nrpys: bOH-Tyr

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAQDIAAVDK
Nearest Stachelhaus code(s):: bOH-Tyr DASTIAAVDK (62% 8Å match)
Stachelhaus code match:: 80% (moderate)

AMP-binding (1329..1728): Pro

nrpys: Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: pro (Pro)

Stachelhaus prediction details:

Stachelhaus sequence:: DVLNLAMVIK
Nearest Stachelhaus code(s):: Ala DVHNLAIVYK (76% 8Å match); Ser DVWNVAMVHK (62% 8Å match)
Stachelhaus code match:: 70% (weak)

zmaF

PKS_AT (5..291): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 62.5%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 51.2
inactive: 31.2
Methoxymalonyl-CoA: 30.8
Methylmalonyl-CoA: 13.8
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Propionyl-CoA: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

zmaJ

Search NORINE for peptide: strict or relaxed

AMP-binding (8..410): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: DVWHFSLIDK
Nearest Stachelhaus code(s):: Ser DVWHFSLIDK (91% 8Å match)
Stachelhaus code match:: 100% (strong)

zmaK

AMP-binding (448..845): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: DMWNTGMVHK
Nearest Stachelhaus code(s):: Ser DMWNTGLVHK (82% 8Å match)
Stachelhaus code match:: 90% (moderate)

PKS_AT (1551..1847): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 133.7
inactive: 95.1
Methoxymalonyl-CoA: 44.7
Methylmalonyl-CoA: 25.8
Propionyl-CoA: 15.1
Isobutyryl-CoA: 9.3
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

zmaO

Search NORINE for peptide: strict or relaxed

AMP-binding (461..856): Asn

nrpys: Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKIGEVGK
Nearest Stachelhaus code(s):: Asn DLTKIGEVGK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

zmaQ

AMP-binding (280..682): X

nrpys: Val, Leu, Ile, Abu, Ival

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVFYFGLVCK
Nearest Stachelhaus code(s):: Met DVFYLGGVFK (59% 8Å match); Ala DVFFVGLVAK (50% 8Å match); Pro DLFYLALVCK (47% 8Å match)
Stachelhaus code match:: 70% (weak)

AMP-binding (1323..1725): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVFYLGGVCK
Nearest Stachelhaus code(s):: Met DVFYLGGVFK (65% 8Å match)
Stachelhaus code match:: 90% (moderate)