BGC0001062 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001062 (Micromonospora echinospora subsp. challisensis)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T2PKS,PKS-like,T1PKS	1	39,835	High	TLN-05220	PKS

BGC0001062 - Region 1 - PKS-like,T1PKS,T2PKS

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Location: 1 - 39,835 nt. (total: 39,835 nt) Show pHMM detection rules used

Region on contig edge.

T2PKS: (t2ks and t2clf)
PKS-like: (t2pks2 or ksIII)
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 39,836
Label		BGC0001062

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase oxygenase

ADB23376.1: FAD dependent oxidoreductase

smcogs: SMCOG1103: FAD dependent oxidoreductase

ADB23387.1: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

ADB23388.1: 3-hydroxyisobutyrate dehydrogenase

smcogs: SMCOG1100: 3-hydroxyisobutyrate dehydrogenase

ADB23395.1: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

ADB23401.1: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

Transferases: acylase kinase methyltransferase

ADB23377.1: glycerol kinase

smcogs: SMCOG1210: glycerol kinase

ADB23379.1: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

ADB23385.1: O-methyltransferase

smcogs: SMCOG1042: O-methyltransferase

Hydrolases: hydrolase

ADB23384.1: asparagine synthase (glutamine-hydrolyzing)

smcogs: SMCOG1177: asparagine synthase (glutamine-hydrolyzing)

Lyases: cyclase

ADB23389.1: Polyketide synthesis cyclase

smcogs: SMCOG1225: Polyketide synthesis cyclase

ADB23394.1: cyclase/dehydrase

smcogs: SMCOG1128: cyclase/dehydrase

Ligases: synthase

ADB23391.1: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

ADB23392.1: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

ADB23398.1: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs: SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

ADB23402.1: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

ADB23403.1: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001062.5	2.85	PKS	TLN-05220	Micromonospora echinospora subsp. challisensis
BGC0000041.3	1.16	PKS	oronofacic acid	Pseudomonas syringae
BGC0001276.3	1.05	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273.3	1.05	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	1.05	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0002191.2	1.04	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001254.3	0.98	PKS	ACT-Toxin II	Alternaria alternata
BGC0001284.4	0.98	PKS	alternariol	Parastagonospora nodorum SN15
BGC0002192.3	0.96	PKS	FR901512	fungal sp. No.14919
BGC0001160.5	0.95	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001062.5	1.00	PKS	TLN-05220	Micromonospora echinospora subsp. challisensis
BGC0000041.3	0.71	PKS	oronofacic acid	Pseudomonas syringae
BGC0001276.3	0.68	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273.3	0.68	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.68	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0002191.2	0.68	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001254.3	0.66	PKS	ACT-Toxin II	Alternaria alternata
BGC0001284.4	0.66	PKS	alternariol	Parastagonospora nodorum SN15
BGC0002192.3	0.66	PKS	FR901512	fungal sp. No.14919
BGC0001160.5	0.65	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Type II PKS

Predicted core structure(s)

For candidate cluster 1, location 0 - 39835:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (Me-mal) + (Me-redmal)
Non-colinear ordering:: ADB23402.1 + ADB23403.1

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 4220 - 39835:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (Me-mal) + (Me-redmal)
Non-colinear ordering:: ADB23402.1 + ADB23403.1

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 10698 - 39835:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (Me-mal) + (Me-redmal)
Non-colinear ordering:: ADB23402.1 + ADB23403.1

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ADB23379.1: mal

PKS_AT (294..583): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 66.7%; inactive: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 45.5
Methylmalonyl-CoA: 25.8
Methoxymalonyl-CoA: 23.3
Propionyl-CoA: 21.1
CHC-CoA: 13.8
2-Methylbutyryl-CoA: 13.4
fatty_acid: 12.5
Ethylmalonyl-CoA: 12.0
inactive: 11.7
Isobutyryl-CoA: 6.0
trans-1,2-CPDA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

ADB23403.1: mmal - mmal

PKS_AT (88..382): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Methoxymalonyl-CoA: 91.7%; Malonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 207.6
Ethylmalonyl-CoA: 194.2
Methoxymalonyl-CoA: 163.9
2-Methylbutyryl-CoA: 133.2
trans-1,2-CPDA: 132.2
Malonyl-CoA: 105.4
Isobutyryl-CoA: 101.2
Propionyl-CoA: 90.3
CHC-CoA: 89.4
Benzoyl-CoA: 65.5
3-Methylbutyryl-CoA: 44.5
Acetyl-CoA: 36.9
inactive: 19.5
fatty_acid: 16.9

PKS_AT (1109..1404): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 210.7
Ethylmalonyl-CoA: 194.1
Methoxymalonyl-CoA: 169.9
2-Methylbutyryl-CoA: 155.2
trans-1,2-CPDA: 136.9
CHC-CoA: 103.8
Malonyl-CoA: 101.9
Propionyl-CoA: 99.6
Isobutyryl-CoA: 90.3
Benzoyl-CoA: 52.4
3-Methylbutyryl-CoA: 47.8
Acetyl-CoA: 31.9
inactive: 28.4
fatty_acid: 25.5

Link to PARAS substrate predictor

Type II PKS gene cluster prediction details

Cluster section:: 0 to 39835
Starter unit(s):: 2-methylbutyryl-CoA (Score: 1938.0; E-value: 0)
malonamyl-CoA (Score: 583.0; E-value: 0)
propionyl-CoA (Score: 111.0; E-value: 3.91e-29)
Malonyl elongations:: 11|12 (Score: 698.3; E-value: 5.7e-213)
Product class(es):: pentangular polyphenol
Product molecular weight(s):: 2-methylbutyryl-CoA_11: 518 Da
2-methylbutyryl-CoA_12: 560 Da
malonamyl-CoA_11: 519 Da
malonamyl-CoA_12: 561 Da
propionyl-CoA_11: 490 Da
propionyl-CoA_12: 532 Da

ADB23379.1: AT (Score: 606.1; E-value: 4e-185); AT ADB23379.1_TLN-05220/TLN-05223_2-methylbutyryl-CoA (Score: 1215.0; E-value: 0.0)
ADB23384.1: AMID (Score: 790.3; E-value: 2.4e-240); AMID ADE34521.1_SF2575_malonamyl-CoA (Score: 583.0; E-value: 0.0)
ADB23385.1: MET C13O|C17O (Score: 505.0; E-value: 1.9e-154)
ADB23387.1: KR C11 (Score: 332.4; E-value: 3.6e-102)
ADB23389.1: CYC C4-C21/C2-C23|C2-C19 (Score: 182.4; E-value: 4e-57)
ADB23390.1: CYC C5-C18 (Score: 222.0; E-value: 4.2e-69)
ADB23391.1: KS (Score: 744.6; E-value: 6.6e-227)
ADB23392.1: CLF 11|12 (Score: 698.3; E-value: 5.7e-213)
ADB23393.1: ACP (Score: 109.2; E-value: 1.8e-34)
ADB23394.1: CYC C9-C14 (Score: 244.6; E-value: 6.8e-76)
ADB23395.1: KR C19 (Score: 405.1; E-value: 2.5e-124)
ADB23398.1: KSIII (Score: 532.4; E-value: 1e-162); KSIII ADB23398.1_TLN-05220/TLN-05223_2-methylbutyryl-CoA (Score: 723.0; E-value: 0.0)
ADB23401.1: OXY (Score: 297.1; E-value: 6.1e-91)
ADB23403.1: AT (Score: 164.1; E-value: 9.9e-51); AT AAF70110.1_Aclacinomycin_propionyl-CoA (Score: 111.0; E-value: 3.91e-29)