BGC0001065 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001065 (Streptomyces chromofuscus)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS	1	86,832	High	herboxidiene	PKS

BGC0001065 - Region 1 - T1PKS

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Location: 1 - 86,832 nt. (total: 86,832 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 86,833
Label		BGC0001065

Tailoring functions

Oxidoreductases: oxygenase

herE: monooxygenase FAD-binding

smcogs: SMCOG1246: monooxygenase FAD-binding

herG: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

Transferases: methyltransferase

herF: methyltransferase

smcogs: SMCOG1089: methyltransferase

Ligases: synthase

herB: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

herC: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

herD: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001065.5	1.00	PKS	herboxidiene	Streptomyces chromofuscus
BGC0001276.3	0.75	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001275.3	0.75	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0002154.2	0.66	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001257.3	0.66	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001284.4	0.66	PKS	alternariol	Parastagonospora nodorum SN15
BGC0001258.3	0.66	PKS	1,3,6,8-tetrahydroxynaphthalene	Glarea lozoyensis
BGC0001164.3	0.64	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001160.5	0.64	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001065.5	1.00	PKS	herboxidiene	Streptomyces chromofuscus
BGC0001276.3	0.75	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001275.3	0.75	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0002154.2	0.66	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001257.3	0.66	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001284.4	0.66	PKS	alternariol	Parastagonospora nodorum SN15
BGC0001258.3	0.66	PKS	1,3,6,8-tetrahydroxynaphthalene	Glarea lozoyensis
BGC0001164.3	0.64	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001160.5	0.64	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 86832:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (Me-mal - pk - pk - pk) + (ccmal - Me-ccmal - pk - redmal) + (ccmal)
Non-colinear ordering:: herB + herC + herD

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

herB: mmal - pk - pk - pk

PKS_AT (514..802): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 214.4
Ethylmalonyl-CoA: 208.8
Methoxymalonyl-CoA: 171.7
2-Methylbutyryl-CoA: 154.4
trans-1,2-CPDA: 151.8
Propionyl-CoA: 120.5
CHC-CoA: 119.7
Malonyl-CoA: 99.7
Isobutyryl-CoA: 76.5
Benzoyl-CoA: 57.1
Acetyl-CoA: 46.4
3-Methylbutyryl-CoA: 39.5
fatty_acid: 34.5
inactive: 27.9

PKS_AT (1531..1838): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Methoxymalonyl-CoA: 91.7%; Malonyl-CoA: 87.5%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 212.4
Methylmalonyl-CoA: 206.9
Methoxymalonyl-CoA: 166.1
2-Methylbutyryl-CoA: 144.1
Propionyl-CoA: 119.2
trans-1,2-CPDA: 118.0
CHC-CoA: 107.1
Malonyl-CoA: 95.0
Isobutyryl-CoA: 82.7
Benzoyl-CoA: 76.9
Acetyl-CoA: 57.3
3-Methylbutyryl-CoA: 52.4
fatty_acid: 40.2
inactive: 19.9

PKS_AT (3017..3310): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 214.9
Methylmalonyl-CoA: 213.7
Methoxymalonyl-CoA: 169.9
2-Methylbutyryl-CoA: 149.6
trans-1,2-CPDA: 120.1
CHC-CoA: 101.0
Malonyl-CoA: 97.4
Propionyl-CoA: 94.1
Benzoyl-CoA: 67.4
Isobutyryl-CoA: 59.1
Acetyl-CoA: 46.2
fatty_acid: 39.8
3-Methylbutyryl-CoA: 32.3
inactive: 20.0

PKS_AT (4804..5090): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 221.2
Methylmalonyl-CoA: 214.6
Methoxymalonyl-CoA: 178.8
2-Methylbutyryl-CoA: 171.0
trans-1,2-CPDA: 121.3
Propionyl-CoA: 117.4
CHC-CoA: 109.9
Malonyl-CoA: 102.2
Isobutyryl-CoA: 87.6
Benzoyl-CoA: 71.7
Acetyl-CoA: 48.0
3-Methylbutyryl-CoA: 41.7
fatty_acid: 30.4
inactive: 26.9

herC: mal - mmal - pk - mal

PKS_AT (528..812): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 75.0%; Propionyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 190.6
inactive: 79.7
Methoxymalonyl-CoA: 77.0
Methylmalonyl-CoA: 76.6
Ethylmalonyl-CoA: 50.7
Isobutyryl-CoA: 36.5
Propionyl-CoA: 26.6
Acetyl-CoA: 22.4
trans-1,2-CPDA: 21.0
CHC-CoA: 20.7
Benzoyl-CoA: 14.9
fatty_acid: 12.3
2-Methylbutyryl-CoA: 9.9
3-Methylbutyryl-CoA: 0.0

PKS_AT (2268..2560): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 202.5
Ethylmalonyl-CoA: 202.0
Methoxymalonyl-CoA: 169.0
2-Methylbutyryl-CoA: 147.9
Propionyl-CoA: 121.3
trans-1,2-CPDA: 118.3
Malonyl-CoA: 112.9
CHC-CoA: 103.1
Isobutyryl-CoA: 92.5
Benzoyl-CoA: 64.7
fatty_acid: 43.3
Acetyl-CoA: 40.5
inactive: 34.4
3-Methylbutyryl-CoA: 21.1

PKS_AT (4005..4300): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 214.9
Methylmalonyl-CoA: 214.4
Methoxymalonyl-CoA: 178.2
2-Methylbutyryl-CoA: 146.3
trans-1,2-CPDA: 130.4
Propionyl-CoA: 119.3
CHC-CoA: 100.1
Malonyl-CoA: 87.7
Isobutyryl-CoA: 81.2
Benzoyl-CoA: 62.4
Acetyl-CoA: 56.2
3-Methylbutyryl-CoA: 42.0
fatty_acid: 35.7
inactive: 26.3

PKS_AT (5541..5839): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 200.9
inactive: 92.6
Methoxymalonyl-CoA: 65.4
Methylmalonyl-CoA: 60.7
Propionyl-CoA: 46.0
Isobutyryl-CoA: 29.5
Acetyl-CoA: 26.8
2-Methylbutyryl-CoA: 26.8
Ethylmalonyl-CoA: 24.0
Benzoyl-CoA: 20.4
CHC-CoA: 17.0
trans-1,2-CPDA: 11.6
fatty_acid: 10.2
3-Methylbutyryl-CoA: 0.0

herD: mal

PKS_AT (568..862): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 70.8%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 219.1
Methoxymalonyl-CoA: 91.3
inactive: 86.3
Methylmalonyl-CoA: 79.1
Isobutyryl-CoA: 48.1
Ethylmalonyl-CoA: 42.9
Propionyl-CoA: 38.6
2-Methylbutyryl-CoA: 30.4
Benzoyl-CoA: 26.3
Acetyl-CoA: 23.0
CHC-CoA: 22.1
fatty_acid: 12.5
trans-1,2-CPDA: 6.1
3-Methylbutyryl-CoA: 0.0

Link to PARAS substrate predictor