BGC0001070: kirromycin biosynthetic gene cluster from Streptomyces collinus Tu 365
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Location: 23,875 - 103,763 nt. (total: 79,889 nt).
This entry is originally from NCBI GenBank AM746336.1.

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
TTA codons
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Gene details
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General
Compounds
Genes
Biosynthesis
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0001070
Short description kirromycin biosynthetic gene cluster from Streptomyces collinus Tu 365
Status Quality: questionable
The quality level of this entry.

Status: active
The status of this entry.

Completeness: complete
Whether the entry covers everything needed for the pathway producing the compound(s)
Biosynthetic class(es)
  • NRPS (Type I)
  • PKS (Type I)
Loci
AM746336.1
23875 - 103763
via Knock-out studies
Compounds
  • kirromycin
Species Streptomyces collinus Tu 365 [taxonomy]
References
Chemical products information
kirromycin [synonyms: delvomycin, mocimycin] Evidence: NMR [1]
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C43H60N2O12
Chemical database entries
PubCHEM
NPAtlas
ChemSpider
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • CAN89630.1
  • kirP
 23876 - 24631 (-) Sfp-type phosphopantetheinyl transferase
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    • CAN89631.1
    • kirAI
    		 25016 - 30997 (+) putative polyketide synthase
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      • CAN89632.1
      • kirAII
      		 31022 - 44176 (+) putative polyketide synthase
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        • CAN89633.1
        • kirAIII
        		 44173 - 48825 (+) putative hybrid non-ribosomal peptide synthetase/polyketide synthase
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          • CAN89634.1
          • kirAIV
          		 48825 - 68021 (+) putative polyketide synthase
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            • CAN89635.1
            • kirAV
            		 68042 - 74902 (+) putative polyketide synthase
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              • CAN89636.1
              • kirAVI
              		 74899 - 83595 (+) cis-AT type-I polyketide synthase
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                • CAN89637.1
                • kirHI
                		 83835 - 84683 (+) Dieckmann cyclase
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                  • CAN89638.1
                  • kirB
                  		 84680 - 87781 (+) putative non-ribosomal peptide synthetase
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                    • CAN89639.1
                    • kirCI
                    		 87805 - 91041 (+) discrete acyl transferase, specific for malonyl-CoA
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                      • CAN89640.1
                      • kirOI
                      		 91056 - 92255 (+) Cytochrome-P450 hydroxylase
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                        • CAN89641.1
                        • kirD
                        		 92270 - 92689 (+) Aspartate-1-decarboxylase precursor
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                          • CAN89642.1
                          • kirM
                          		 92721 - 93677 (+) SAM-dependent methyltransferase
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                            • CAN89643.1
                            • kirCII
                            		 93687 - 95024 (+) discrete acyltransferase, specific for ethylmalonyl-CoA
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                              • CAN89644.1
                              • kirHII
                              		 95016 - 95573 (-) hypothetical protein
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                                • CAN89645.1
                                • kirHIII
                                		 95570 - 95902 (-) hypothetical protein
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                                  • CAN89646.1
                                  • kirTI
                                  		 95954 - 96856 (-) putative major facilitator family transporter
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                                    • CAN89647.1
                                    • kirTII
                                    		 96872 - 97618 (-) putative major facilitator family transporter
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                                      • CAN89648.1
                                      • kirRI
                                      		 97676 - 97990 (-) putative TetR family transcriptional regulator
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                                        • CAN89649.1
                                        • kirRII
                                        		 98024 - 98383 (-) putative TetR family transcriptional regulator
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                                          • CAN89650.1
                                          • kirOII
                                          		 98503 - 99720 (+) Cytochrome-P450 hydroxylase
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                                            • CAN89651.1
                                            • kirHIV
                                            		 99761 - 100543 (+) hypothetical protein
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                                              • CAN89652.1
                                              • kirHV
                                              		 100540 - 100935 (+) PhyH family dioxygenase
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                                                • CAN89653.1
                                                • kirN
                                                		 100994 - 102361 (+) putative crotonyl-CoA reductase
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                                                  • CAN89654.1
                                                  • kirHVI
                                                  		 102358 - 103203 (+) hypothetical protein
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                                                    • CAN89655.1
                                                    • kirE
                                                    		 103254 - 103763 (-) putative GNAT family acetyltransferase
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                                                      Biosynthesis information

                                                      Biosynthetic modules

                                                      Name
                                                      6
                                                      Type
                                                      nrps-type1
                                                      Genes
                                                      kirAIII
                                                      Substrates
                                                      glycine (evidence: Structure-based inference)
                                                      Integrated Monomers
                                                      Domains
                                                      condensation (LCL), adenylation
                                                      Name
                                                      16
                                                      Type
                                                      nrps-type1
                                                      Genes
                                                      kirB
                                                      Substrates
                                                      beta-alanine (evidence: Structure-based inference)
                                                      Integrated Monomers
                                                      Domains
                                                      condensation (LCL), adenylation
                                                      Name
                                                      0
                                                      Type
                                                      pks-trans-at
                                                      Genes
                                                      kirAI
                                                      Substrates
                                                      Integrated Monomers
                                                      Domains
                                                      ketosynthase, carrier (ACP)
                                                      Name
                                                      1
                                                      Type
                                                      pks-trans-at
                                                      Genes
                                                      kirAI
                                                      Substrates
                                                      Integrated Monomers
                                                      Domains
                                                      ketosynthase, ketoreductase, dehydratase, carrier (ACP)
                                                      Name
                                                      2
                                                      Type
                                                      pks-trans-at
                                                      Genes
                                                      kirAI kirAII
                                                      Substrates
                                                      Integrated Monomers
                                                      Domains
                                                      ketosynthase, ketoreductase, dehydratase, carrier (ACP)
                                                      Name
                                                      3
                                                      Type
                                                      pks-trans-at
                                                      Genes
                                                      kirAII
                                                      Substrates
                                                      Integrated Monomers
                                                      Domains
                                                      ketosynthase, ketoreductase, methyltransferase, carrier (ACP)
                                                      Name
                                                      4
                                                      Type
                                                      pks-trans-at
                                                      Genes
                                                      kirAII
                                                      Substrates
                                                      Integrated Monomers
                                                      Domains
                                                      ketosynthase, ketoreductase, carrier (ACP)
                                                      Name
                                                      5
                                                      Type
                                                      pks-trans-at
                                                      Genes
                                                      kirAII
                                                      Substrates
                                                      Integrated Monomers
                                                      Domains
                                                      ketosynthase, carrier (ACP)
                                                      Name
                                                      7
                                                      Type
                                                      pks-trans-at
                                                      Genes
                                                      kirAIII kirAIV
                                                      Substrates
                                                      Integrated Monomers
                                                      Domains
                                                      ketosynthase, ketoreductase, dehydratase, carrier (ACP)
                                                      Name
                                                      8
                                                      Type
                                                      pks-trans-at
                                                      Genes
                                                      kirAIV
                                                      Substrates
                                                      Integrated Monomers
                                                      Domains
                                                      ketosynthase, ketoreductase, dehydratase, methyltransferase, carrier (ACP)
                                                      Name
                                                      9
                                                      Type
                                                      pks-trans-at
                                                      Genes
                                                      kirAIV
                                                      Substrates
                                                      Integrated Monomers
                                                      Domains
                                                      ketosynthase, carrier (ACP)
                                                      Name
                                                      10
                                                      Type
                                                      pks-trans-at
                                                      Genes
                                                      kirAIV
                                                      Substrates
                                                      Integrated Monomers
                                                      Domains
                                                      ketosynthase, ketoreductase, carrier (ACP)
                                                      Name
                                                      11
                                                      Type
                                                      pks-trans-at
                                                      Genes
                                                      kirAIV
                                                      Substrates
                                                      Integrated Monomers
                                                      Domains
                                                      ketosynthase, ketoreductase, carrier (ACP)
                                                      Name
                                                      12
                                                      Type
                                                      pks-trans-at
                                                      Genes
                                                      kirAIV kirAV
                                                      Substrates
                                                      Integrated Monomers
                                                      Domains
                                                      ketosynthase, ketoreductase, dehydratase, carrier (ACP)
                                                      Name
                                                      13
                                                      Type
                                                      pks-trans-at
                                                      Genes
                                                      kirAV
                                                      Substrates
                                                      Integrated Monomers
                                                      Domains
                                                      ketosynthase, ketoreductase, dehydratase, carrier (ACP)
                                                      Name
                                                      14
                                                      Type
                                                      pks-modular
                                                      Genes
                                                      kirAVI
                                                      Substrates
                                                      Integrated Monomers
                                                      Domains
                                                      acyltransferase, ketosynthase, acyltransferase, ketoreductase, dehydratase, carrier (ACP)
                                                      Name
                                                      15
                                                      Type
                                                      pks-modular
                                                      Genes
                                                      kirAVI
                                                      Substrates
                                                      Integrated Monomers
                                                      Domains
                                                      acyltransferase, ketosynthase, acyltransferase, carrier (ACP)
                                                      Annotation changelog

                                                      Entry version: next

                                                      Date
                                                      Changes
                                                      Submitters
                                                      Reviewers
                                                      2024-03-19
                                                      MIBiG v4 annotathon
                                                      • (ID: I3ASMMTCDAWWX3V7AIOZWTR2)
                                                      • (ID: CUIZDUBUBVAF27VHF4OZXTI3)
                                                      • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                                                      Entry version: 4

                                                      Date
                                                      Changes
                                                      Submitters
                                                      Reviewers
                                                      2022-10-07
                                                      Update chemical activity to schema version 2.11
                                                      • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                      • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                                                      Entry version: 3

                                                      Date
                                                      Changes
                                                      Submitters
                                                      Reviewers
                                                      2022-09-15
                                                      Corrected NRP module activity
                                                      • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                      • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                      2022-09-15
                                                      Removed duplicate gene annotation
                                                      • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                      • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                      2022-09-15
                                                      Removed ketoreductase stereochemistry annotation from modules without ketoreductases
                                                      • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                      • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                      2022-09-15
                                                      Merged cross-CDS PKS modules
                                                      • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                      • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                      2022-09-15
                                                      Excluded flanking genes
                                                      • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                      • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                      2022-09-15
                                                      Updated bioactivity data
                                                      • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                      • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                      2022-09-15
                                                      Updated NRP substrate specificities
                                                      • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                      • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                                                      Entry version: 2

                                                      Date
                                                      Changes
                                                      Submitters
                                                      Reviewers
                                                      2019-10-16
                                                      Migrated from v1.4
                                                      • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                      • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                                                      Entry version: 1

                                                      Date
                                                      Changes
                                                      Submitters
                                                      Reviewers
                                                      2015-06-12
                                                      Submitted
                                                      • (ID: I3ASMMTCDAWWX3V7AIOZWTR2)
                                                      • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                      Detailed domain annotation
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