BGC0001070: kirromycin biosynthetic gene cluster from Streptomyces collinus Tu 365
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Location: 1 - 132,542 nt. (total: 132,542 nt).
This entry is originally from NCBI GenBank AM746336.1.

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Gene details
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General
Compounds
Genes
Polyketide
NRP
History
KnownClusterBlast
NRPS/PKS domains
General information about the BGC
MIBiG accession BGC0001070
Short description kirromycin biosynthetic gene cluster from Streptomyces collinus Tu 365
Status Minimal annotation: no
A minimal annotation only contains information on the BGC loci and one or more linked chemical product(s)

Completeness: complete
Whether the loci encodes everything needed for the pathway producing the compound(s)
Biosynthetic class(es)
  • NRP
  • Polyketide (Other)
Loci NCBI GenBank: AM746336.1
Compounds
  • kirromycin
Species Streptomyces collinus Tu 365 [taxonomy]
References
Chemical products information
kirromycin [synonyms: mocimycin, delvomycin]
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C43H60N2O12
Chemical database entries
PubCHEM
ChemSpider
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • orf_L20
  • CAN89610.1
1 - 1172 (-) hypothetical protein
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  • orf_L19
  • CAN89611.1
1188 - 2030 (-) hypothetical protein
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  • orf_L18
  • CAN89612.1
2246 - 2887 (-) putative uridine kinase
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  • orf_L17
  • CAN89613.1
3495 - 5123 (+) hypothetical protein
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  • orf_L16
  • CAN89614.1
5488 - 6513 (+) putative AraC family transcriptional regulator
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  • orf_L15
  • CAN89615.1
6793 - 7845 (+) hypothetical protein
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  • orf_L14
  • CAN89616.1
7934 - 8908 (+) putative zinc-containing alcolhol dehydrogenase
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  • orf_L13
  • CAN89617.1
8964 - 9887 (+) putative taurine dioxygenase
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  • orf_L12
  • CAN89618.1
9930 - 11348 (+) putative major facilitator superfamily transporter
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  • orf_L11
  • CAN89619.1
11620 - 12039 (-) putative HTH-type regulator
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  • orf_L10
  • CAN89620.1
12143 - 12994 (+) putative hydrolase
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  • orf_L9
  • CAN89621.1
13163 - 14311 (-) hypothetical protein
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  • orf_L8
  • CAN89622.1
14631 - 16265 (+) putative major facilitator superfamily transporter
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  • orf_L7
  • CAN89623.1
16407 - 17144 (+) hypothetical protein
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  • orf_L6
  • CAN89624.1
18506 - 19639 (+) putative nucleoside-diphosphate sugar epimerase
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  • orf_L5
  • CAN89625.1
19636 - 20838 (+) putative glycosyltransferase
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  • orf_L4
  • CAN89626.1
20932 - 21699 (-) putative metallo-beta-lactamase family protein
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  • orf_L3
  • CAN89627.1
21765 - 22031 (+) putative zinc dependent dehydrogenase
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  • orf_L2
  • CAN89628.1
22050 - 22592 (-) putative lipoprotein
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  • orf_L1
  • CAN89629.1
22893 - 23399 (+) hypothetical protein
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  • CAN89630.1
  • kirP
23876 - 24631 (-) putative Sfp type phosphopantetheinyl transferase
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  • CAN89631.1
  • kirAI
25016 - 30997 (+) putative polyketide synthase
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  • CAN89632.1
  • kirAII
31022 - 44176 (+) putative polyketide synthase
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  • CAN89633.1
  • kirAIII
44173 - 48825 (+) putative hybrid non-ribosomal peptide synthetase/polyketide synthase
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  • CAN89634.1
  • kirAIV
48825 - 68021 (+) putative polyketide synthase
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  • CAN89635.1
  • kirAV
68042 - 74902 (+) putative polyketide synthase
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  • CAN89636.1
  • kirAVI
74899 - 83595 (+) putative polyketide synthase
  • Scaffold biosynthesis
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  • CAN89637.1
  • kirHI
83835 - 84683 (+) hypothetical protein
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  • CAN89638.1
  • kirB
84680 - 87781 (+) putative non-ribosomal peptide synthetase
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  • CAN89639.1
  • kirCI
87805 - 91041 (+) putative acyltransferase
  • Scaffold biosynthesis
Activity assay
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  • CAN89640.1
  • kirOI
91056 - 92255 (+) putative cytochrome P450 hydroxylase
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  • CAN89641.1
  • kirD
92270 - 92689 (+) putative aspartate-1-decarboxylase precursor
  • Unknown
Activity assay
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  • CAN89642.1
  • kirM
92721 - 93677 (+) putative SAM-dependent methyltransferase
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  • CAN89643.1
  • kirCII
93687 - 95024 (+)
  • Scaffold biosynthesis
Activity assay
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  • CAN89644.1
  • kirHII
95016 - 95573 (-) hypothetical protein
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  • CAN89645.1
  • kirHIII
95570 - 95902 (-) hypothetical protein
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  • CAN89646.1
  • kirTI
95954 - 96856 (-) putative major facilitator family transporter
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  • CAN89647.1
  • kirTII
96872 - 97618 (-) putative major facilitator family transporter
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  • CAN89648.1
  • kirRI
97676 - 97990 (-) putative TetR family transcriptional regulator
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  • CAN89649.1
  • kirRII
98024 - 98383 (-) putative TetR family transcriptional regulator
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  • CAN89650.1
  • kirOII
98503 - 99720 (+) putative cytochrome P450 hydroxylase
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  • CAN89651.1
  • kirHIV
99761 - 100543 (+) hypothetical protein
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  • CAN89652.1
  • kirHV
100540 - 100935 (+) hypothetical protein
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  • CAN89653.1
  • kirN
100994 - 102361 (+) putative crotonyl-CoA reductase
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  • CAN89654.1
  • kirHVI
102358 - 103203 (+) hypothetical protein
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  • CAN89655.1
  • kirE
103254 - 103763 (-) putative GNAT family acetyltransferase
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  • orf_R1
  • CAN89656.1
105617 - 113008 (+) putative non-ribosomal peptide synthetase
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  • orf_R2
  • CAN89657.1
113005 - 114624 (+) putative argininosuccinate lyase
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  • orf_R3
  • CAN89658.1
114624 - 115607 (+) hypothetical protein
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  • orf_R4
  • CAN89659.1
115610 - 117883 (+) putative pyridoxal-phosphate dependent enzyme
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  • orf_R5
  • CAN89660.1
117976 - 118200 (+) putative MbtH-like protein
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  • orf_R6
  • CAN89661.1
118193 - 120250 (+) putative non-ribosomal peptide synthetase
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  • orf_R7
  • CAN89662.1
120247 - 123468 (+) putative non-ribosomal peptide synthetase
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  • orf_R8
  • CAN89663.1
123771 - 127067 (+) putative non-ribosomal peptide synthetase
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  • orf_R9
  • CAN89664.1
127105 - 128673 (+) putative amino acid adenylating enzyme
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  • orf_R10
  • CAN89665.1
128736 - 130043 (+) putative major facilitator family transporter
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  • orf_R11
  • CAN89666.1
130037 - 130366 (-) putative LuxR family regulator
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  • orf_R12
  • CAN89667.1
130516 - 131550 (+) putative LuxR family regulator
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  • orf_R13
  • CAN89668.1
131952 - 132479 (+) hypothetical protein
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  • CAN89641.1
(external)
  • Precursor biosynthesis
Activity assay
Polyketide-specific information
Subclass Other
Starter unit Acetyl-CoA
Cyclic? no
Polyketide-synthases
Genes Properties Modules
CAN89632.1
+
CAN89633.1
+
CAN89639.1
+
CAN89643.1
+
CAN89634.1
+
CAN89635.1
+
CAN89631.1
+
CAN89636.1
Synthase subclass: Modular type I/Trans-AT type I
Trans-acyltansferases: CAN89639.1, CAN89643.1
Module 0
Genes: CAN89631.1
Core domains: Ketosynthase, Thiolation (ACP/PCP)
Module 1
Genes: CAN89631.1
Core domains: Ketosynthase, Ketoreductase, Dehydratase, Thiolation (ACP/PCP)
Module 2
Genes: CAN89631.1
Core domains: Ketosynthase
Module 2
Genes: CAN89632.1
Core domains: Ketoreductase, Dehydratase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
Module 3
Genes: CAN89632.1
Core domains: Ketosynthase, Ketoreductase, Thiolation (ACP/PCP)
Scaffold-modifying domains: Methylation
KR-domain stereochemistry: D-OH
Module 4
Genes: CAN89632.1
Core domains: Ketosynthase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
Module 5
Genes: CAN89632.1
Core domains: Ketosynthase, Thiolation (ACP/PCP)
Module 7
Genes: CAN89633.1
Core domains: Ketosynthase
Module 7
Genes: CAN89634.1
Core domains: Ketoreductase, Dehydratase, Thiolation (ACP/PCP)
KR-domain stereochemistry: D-OH
Module 8
Genes: CAN89634.1
Core domains: Ketosynthase, Ketoreductase, Dehydratase, Thiolation (ACP/PCP)
Scaffold-modifying domains: Methylation
KR-domain stereochemistry: L-OH
Module 9
Genes: CAN89634.1
Core domains: Ketosynthase, Thiolation (ACP/PCP)
Module 10
Genes: CAN89634.1
Core domains: Ketosynthase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: D-OH
Module 11
Genes: CAN89634.1
Core domains: Ketosynthase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
Module 12
Genes: CAN89634.1
Core domains: Ketosynthase
Module 12
Genes: CAN89635.1
Core domains: Ketoreductase, Dehydratase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
Module 13
Genes: CAN89635.1
Core domains: Ketosynthase, Ketoreductase, Dehydratase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
Module 14
Specificity: Methylmalonyl-CoA
Genes: CAN89636.1
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Dehydratase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
Module 15
Specificity: Malonyl-CoA
Genes: CAN89636.1
Core domains: Ketosynthase, Acyltransferase, Thiolation (ACP/PCP)
NRP-specific information
Subclass n/a
Cyclic? no
NRP-synthases
Gene Modules
CAN89633.1
Module 6
Specificity: Glycine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: LCL
CAN89638.1
Module 16
Specificity: Beta-alanine
Evidence for specificity:
  • Structure-based inference
Genes encoding specified AA: CAN89641.1
Condensation domain type: LCL
Annotation changelog
MIBiG version Submitter Notes
1.0
  • Hidden contributor (ID: I3ASMMTCDAWWX3V7AIOZWTR2, no GDPR consent given).
  • Submitted
2.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Migrated from v1.4
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