BGC0001070: kirromycin biosynthetic gene cluster from Streptomyces collinus Tu 365
Shows the layout of the region, marking coding sequences and areas of interest. Clicking a gene will select it and show any relevant details. Clicking an area feature (e.g. a candidate cluster) will select all coding sequences within that area. Double clicking an area feature will zoom to that area. Multiple genes and area features can be selected by clicking them while holding the Ctrl key.
View antiSMASH-generated output
Download Cluster GenBank file
Download region SVG
Location: 23,875 - 103,763 nt. (total: 79,889 nt).
This entry is originally from NCBI GenBank AM746336.1.

Legend:

core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
TTA codons
reset zoomreset view
zoomzoom to selection
Gene details
Shows details of the most recently selected gene, including names, products, location, and other annotations.
Select a gene to view the details available for it
General
Compounds
Genes
Polyketide
NRP
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0001070
Short description kirromycin biosynthetic gene cluster from Streptomyces collinus Tu 365
Status Minimal annotation: no
A minimal annotation only contains information on the BGC loci and one or more linked chemical product(s)

Completeness: complete
Whether the loci encodes everything needed for the pathway producing the compound(s)
Biosynthetic class(es)
  • NRP
  • Polyketide (Other)
Loci NCBI GenBank: AM746336.1
Compounds
  • kirromycin
Species Streptomyces collinus Tu 365 [taxonomy]
References
Chemical products information
kirromycin [synonyms: mocimycin, delvomycin]
Copy SMILES
C43H60N2O12
Chemical database entries
PubCHEM
ChemSpider
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • CAN89630.1
  • kirP
 23876 - 24631 (-) putative Sfp type phosphopantetheinyl transferase
copy AA seq
copy Nt seq
  • CAN89631.1
  • kirAI
 25016 - 30997 (+) putative polyketide synthase
copy AA seq
copy Nt seq
  • CAN89632.1
  • kirAII
 31022 - 44176 (+) putative polyketide synthase
copy AA seq
copy Nt seq
  • CAN89633.1
  • kirAIII
 44173 - 48825 (+) putative hybrid non-ribosomal peptide synthetase/polyketide synthase
copy AA seq
copy Nt seq
  • CAN89634.1
  • kirAIV
 48825 - 68021 (+) putative polyketide synthase
copy AA seq
copy Nt seq
  • CAN89635.1
  • kirAV
 68042 - 74902 (+) putative polyketide synthase
copy AA seq
copy Nt seq
  • CAN89636.1
  • kirAVI
 74899 - 83595 (+) putative polyketide synthase
  • Scaffold biosynthesis
Knock-out
copy AA seq
copy Nt seq
  • CAN89637.1
  • kirHI
 83835 - 84683 (+) hypothetical protein
copy AA seq
copy Nt seq
  • CAN89638.1
  • kirB
 84680 - 87781 (+) putative non-ribosomal peptide synthetase
copy AA seq
copy Nt seq
  • CAN89639.1
  • kirCI
 87805 - 91041 (+) putative acyltransferase
  • Scaffold biosynthesis
Activity assay
copy AA seq
copy Nt seq
  • CAN89640.1
  • kirOI
 91056 - 92255 (+) putative cytochrome P450 hydroxylase
copy AA seq
copy Nt seq
  • CAN89641.1
  • kirD
 92270 - 92689 (+) putative aspartate-1-decarboxylase precursor
  • Precursor biosynthesis
Activity assay
copy AA seq
copy Nt seq
  • CAN89642.1
  • kirM
 92721 - 93677 (+) putative SAM-dependent methyltransferase
copy AA seq
copy Nt seq
  • CAN89643.1
  • kirCII
 93687 - 95024 (+)
  • Scaffold biosynthesis
Activity assay
copy AA seq
copy Nt seq
  • CAN89644.1
  • kirHII
 95016 - 95573 (-) hypothetical protein
copy AA seq
copy Nt seq
  • CAN89645.1
  • kirHIII
 95570 - 95902 (-) hypothetical protein
copy AA seq
copy Nt seq
  • CAN89646.1
  • kirTI
 95954 - 96856 (-) putative major facilitator family transporter
copy AA seq
copy Nt seq
  • CAN89647.1
  • kirTII
 96872 - 97618 (-) putative major facilitator family transporter
copy AA seq
copy Nt seq
  • CAN89648.1
  • kirRI
 97676 - 97990 (-) putative TetR family transcriptional regulator
copy AA seq
copy Nt seq
  • CAN89649.1
  • kirRII
 98024 - 98383 (-) putative TetR family transcriptional regulator
copy AA seq
copy Nt seq
  • CAN89650.1
  • kirOII
 98503 - 99720 (+) putative cytochrome P450 hydroxylase
copy AA seq
copy Nt seq
  • CAN89651.1
  • kirHIV
 99761 - 100543 (+) hypothetical protein
copy AA seq
copy Nt seq
  • CAN89652.1
  • kirHV
 100540 - 100935 (+) hypothetical protein
copy AA seq
copy Nt seq
  • CAN89653.1
  • kirN
 100994 - 102361 (+) putative crotonyl-CoA reductase
copy AA seq
copy Nt seq
  • CAN89654.1
  • kirHVI
 102358 - 103203 (+) hypothetical protein
copy AA seq
copy Nt seq
  • CAN89655.1
  • kirE
 103254 - 103763 (-) putative GNAT family acetyltransferase
copy AA seq
copy Nt seq
Polyketide-specific information
Subclass Other
Starter unit Acetyl-CoA
Cyclic? no
Polyketide-synthases
Genes Properties Modules
kirAII
+
kirAIII
+
kirCI
+
kirCII
+
kirAIV
+
kirAV
+
kirAI
+
kirAVI 		Synthase subclass: Modular type I/Trans-AT type I
Trans-acyltansferases: kirCI, kirCII
Module 0
Genes: kirAI
Core domains: Ketosynthase, Thiolation (ACP/PCP)
Module 1
Genes: kirAI
Core domains: Ketosynthase, Ketoreductase, Dehydratase, Thiolation (ACP/PCP)
KR-domain stereochemistry: Unknown
Module 2
Genes: kirAI, kirAII
Core domains: Ketosynthase, Ketoreductase, Dehydratase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
Module 3
Genes: kirAII
Core domains: Ketosynthase, Ketoreductase, Thiolation (ACP/PCP)
Scaffold-modifying domains: Methylation
KR-domain stereochemistry: D-OH
Module 4
Genes: kirAII
Core domains: Ketosynthase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
Module 5
Genes: kirAII
Core domains: Ketosynthase, Thiolation (ACP/PCP)
Module 7
Genes: kirAIII, kirAIV
Core domains: Ketosynthase, Ketoreductase, Dehydratase, Thiolation (ACP/PCP)
KR-domain stereochemistry: D-OH
Module 8
Genes: kirAIV
Core domains: Ketosynthase, Ketoreductase, Dehydratase, Thiolation (ACP/PCP)
Scaffold-modifying domains: Methylation
KR-domain stereochemistry: L-OH
Module 9
Genes: kirAIV
Core domains: Ketosynthase, Thiolation (ACP/PCP)
Module 10
Genes: kirAIV
Core domains: Ketosynthase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: D-OH
Module 11
Genes: kirAIV
Core domains: Ketosynthase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
Module 12
Genes: kirAIV, kirAV
Core domains: Ketosynthase, Ketoreductase, Dehydratase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
Module 13
Genes: kirAV
Core domains: Ketosynthase, Ketoreductase, Dehydratase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
Module 14
Specificity: Methylmalonyl-CoA
Genes: kirAVI
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Dehydratase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
Module 15
Specificity: Malonyl-CoA
Genes: kirAVI
Core domains: Ketosynthase, Acyltransferase, Thiolation (ACP/PCP)
NRP-specific information
Subclass N/A
Cyclic? no
NRP-synthases
Gene Modules
kirAIII
Module 6
Specificity: glycine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: LCL
kirB
Module 16
Specificity: beta-alanine
Evidence for specificity:
  • Structure-based inference
Genes encoding specified AA: kirD
Condensation domain type: LCL
Annotation changelog
MIBiG version Submitter Notes
1.0
  • Hidden contributor (ID: I3ASMMTCDAWWX3V7AIOZWTR2, no GDPR consent given).
  • Submitted
2.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Migrated from v1.4
3.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Corrected NRP module activity
  • Removed duplicate gene annotation
  • Removed ketoreductase stereochemistry annotation from modules without ketoreductases
  • Merged cross-CDS PKS modules
  • Excluded flanking genes
  • Updated bioactivity data
  • Updated NRP substrate specificities
3.1
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Update chemical activity to schema version 2.11
Detailed domain annotation
Shows NRPS- and PKS-related domains for each feature that contains them. Click on each domain for more information about the domain's location, consensus monomer prediction, and other details.
A glossary is available here.
Selected features only
Show module domains
Similar known gene clusters
Shows clusters from the MiBIG database that are similar to the current region. Genes marked with the same colour are interrelated. White genes have no relationship.
Click on reference genes to show details of similarities to genes within the current region.
Click on an accession to open that entry in the MiBIG database.