Genomic loci for this biosynthetic pathway

Cluster Type From To
The following clusters are from record BGC0001082.1:
Cluster 1Terpene118809

BGC0001082, paxilline biosynthetic gene cluster from Penicillium paxilli. Locus 1. Full MIBiG entry.

Chemical compounds

Compound: paxilline
PubChem ID: 105008
SMILES string: Copy to clipboard
Molecular formula: C27H33NO4
Average molecular mass: 435.564 Da
Molecular activity: Inhibitor
Molecular target: BK channels

Class-specific details

Biosynthetic class(es):
Terpene
Terpene subclass:
Sterol
Terpene subclass by size:
Hemiterpene
Final isoprenoid precursor:
DMAPP
Terpene synthase/cyclase-encoding genes:

Gene cluster description

paxilline (BGC0001082). Gene Cluster 1. Biosynthetic class = Terpene. GenBank HM171111, positions 28174-46982. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Homologous known gene clusters

General MIBiG information on this cluster

Complete gene cluster sequence?complete
Evidence for cluster-compound connection:Knock-out studies, Sequence-based prediction
MIxS-compliance:Unknown
Contact for this cluster:Barry Scott (Massey University)

Literature references

1. Young C et al. (2001) Molecular cloning and genetic analysis of an indole-diterpene gene cluster from Penicillium paxilli. Mol Microbiol 39(3):754-64.
2. Saikia S et al. (2006) Four gene products are required for the fungal synthesis of the indole-diterpene, paspaline. FEBS Lett 580(6):1625-30. doi: 10.1016/j.febslet.2006.02.008. Epub
3. Saikia S et al. (2007) Defining paxilline biosynthesis in Penicillium paxilli: functional characterization of two cytochrome P450 monooxygenases. J Biol Chem 282(23):16829-37. doi: 10.1074/jbc.M701626200. Epub 2007
4. Saikia S, Scott B. (2009) Functional analysis and subcellular localization of two geranylgeranyl diphosphate synthases from Penicillium paxilli. Mol Genet Genomics 282(3):257-71. doi: 10.1007/s00438-009-0463-5. Epub
5. Scott B et al. (2013) Deletion and gene expression analyses define the paxilline biosynthetic gene cluster in Penicillium paxilli. Toxins (Basel) 5(8):1422-46. doi: 10.3390/toxins5081422.
6. Tagami K et al. (2013) Reconstitution of biosynthetic machinery for indole-diterpene paxilline in Aspergillus oryzae. J Am Chem Soc 135(4):1260-3. doi: 10.1021/ja3116636. Epub 2013 Jan
7. Liu C et al. (2014) Functional analysis of a prenyltransferase gene (paxD) in the paxilline biosynthetic gene cluster. Appl Microbiol Biotechnol 98(1):199-206. doi:
8. Fan Y et al. (2013) Indole-diterpenoids with anti-H1N1 activity from the aciduric fungus Penicillium camemberti OUCMDZ-1492. J Nat Prod 76(7):1328-36. doi: 10.1021/np400304q. Epub 2013 Jul 2.