BGC0001083: merochlorin A biosynthetic gene cluster from Streptomyces sp. CNH189
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Location: 1 - 60,157 nt. (total: 60,157 nt).
This entry is originally from NCBI GenBank JX186999.1.

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
TTA codons
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Gene details
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General
Compounds
Genes
Biosynthesis
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0001083
Short description merochlorin A biosynthetic gene cluster from Streptomyces sp. CNH189
Status Quality: questionable
The quality level of this entry.

Status: active
The status of this entry.

Completeness: complete
Whether the entry covers everything needed for the pathway producing the compound(s)
Remarks "An unusual terpene cyclization catalyzed by chloroperoxidases not by classic terpene synthase/cyclases"
Biosynthetic class(es)
  • PKS (Type III)
  • terpene (Sesquiterpene)
Loci
JX186999.1
via Knock-out studies, Enzymatic assays, Heterologous expression
Compounds
  • merochlorin A
  • merochlorin B
  • deschloro-merochlorin A
  • deschloro-merochlorin B
  • isochloro-merochlorin B
  • dichloro-merochlorin B
  • merochlorin D
  • merochlorin C
Species Streptomyces sp. CNH189 [taxonomy]
References
Chemical products information
merochlorin A Evidence:
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C25H31Cl1O4
Chemical database entries
NPAtlas
merochlorin B Evidence:
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C25H29Cl1O4
Chemical database entries
NPAtlas
deschloro-merochlorin A Evidence:
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C25H30O4
deschloro-merochlorin B Evidence:
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C25H30O4
isochloro-merochlorin B Evidence:
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C25H29Cl1O4
dichloro-merochlorin B Evidence:
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C25H28Cl2O4
merochlorin D Evidence:
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C26H33Cl1O5
Chemical database entries
NPAtlas
merochlorin C Evidence:
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C26H32Cl2O5
Chemical database entries
NPAtlas
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • AGH68886.1
  • mcl1
 1 - 858 (-) methylenetetrahydrofolate reductase
  • Precursor biosynthesis
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  • AGH68887.1
  • mcl2
 1096 - 2169 (-) isoprenyl diphosphate synthase
  • Precursor biosynthesis
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  • AGH68888.1
  • mcl3
 2291 - 3268 (-) ketoacyl-ACP synthase III
  • Precursor biosynthesis
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  • AGH68889.1
  • mcl4
 3403 - 4578 (-) HMG-CoA synthase
  • Precursor biosynthesis
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  • AGH68890.1
  • mcl5
 4582 - 5643 (-) HMG-CoA reductase
  • Precursor biosynthesis
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  • AGH68891.1
  • mcl6
 5636 - 6730 (-) isopentenyldiphosphate isomerase
  • Precursor biosynthesis
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  • AGH68892.1
  • mcl7
 6727 - 7839 (-) phosphomevalonate kinase
  • Precursor biosynthesis
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  • AGH68893.1
  • mcl8
 7836 - 8876 (-) mevalonate diphosphate decarboxylase
  • Precursor biosynthesis
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  • AGH68894.1
  • mcl9
 8866 - 9903 (-) mevalonate kinase
  • Precursor biosynthesis
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  • AGH68895.1
  • mcl10
 10838 - 11023 (-) MerR-like transcriptional regulator
  • Regulation
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  • AGH68896.1
  • mcl11
 11020 - 11649 (-) hypothetical protein
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    • AGH68897.1
    • mcl12
    		 12224 - 14245 (+) oxidoreductase
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      • AGH68898.1
      • mcl13
      		 14242 - 15681 (+) 3-carboxymuconate cycloisomerase
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        • AGH68899.1
        • mcl14
        		 16151 - 17692 (+) FAD-dependent oxidoreductase
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          • AGH68900.1
          • mcl15
          		 18104 - 19804 (+) cytochrome B-like protein
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            • AGH68901.1
            • mcl16
            		 20057 - 20416 (+) hypothetical protein
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              • AGH68902.1
              • mcl17
              		 21073 - 22149 (+) type-III polyketide synthase
              • Scaffold biosynthesis
              • Activity assay
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              • AGH68903.1
              • mcl18
              		 22146 - 22706 (+) monooxygenase
              • Tailoring
              • Knock-out
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              • AGH68904.1
              • mcl19
              		 22712 - 23878 (+) aminotransferase
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                • AGH68905.1
                • mcl20
                		 24166 - 25752 (+) AMP-dependent synthetase
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                  • AGH68906.1
                  • mcl21
                  		 25749 - 26882 (+) methyltransferase
                  • Tailoring
                  • Knock-out
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                  • AGH68907.1
                  • mcl22
                  		 27097 - 27750 (+) isosesquilavandulyl diphosphate synthase
                  • Precursor biosynthesis
                  • Activity assay
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                  • AGH68908.1
                  • mcl23
                  		 28223 - 29140 (+) aromatic prenyltransferase
                  • Tailoring
                  • Activity assay
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                  • AGH68909.1
                  • mcl24
                  		 29301 - 30854 (+) vanadium-dependent chloroperoxidase
                  • Tailoring
                  • Activity assay
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                  • AGH68910.1
                  • mcl25
                  		 30953 - 32515 (+) MFS-like transporter
                  • Transport
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                  • AGH68911.1
                  • mcl26
                  		 32659 - 33612 (-) aromatase
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                    • AGH68912.1
                    • mcl27
                    		 33703 - 34425 (-) thioesterase
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                      • AGH68913.1
                      • mcl28
                      		 34587 - 35408 (-) SARP-like transcriptional regulator
                      • Regulation
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                      • AGH68914.1
                      • mcl29
                      		 35748 - 36416 (-) TetR-like transcriptional regulator
                      • Regulation
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                      • AGH68915.1
                      • mcl30
                      		 36568 - 38886 (-) iron-sulfur-binding oxidoreductase
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                        • AGH68916.1
                        • mcl31
                        		 39033 - 39902 (-) SARP-like transcriptional regulator
                        • Regulation
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                        • AGH68917.1
                        • mcl32
                        		 40410 - 41057 (-) TetR-like transcriptional regulator
                        • Regulation
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                        • AGH68918.1
                        • mcl33
                        		 41565 - 43022 (-) NRPS-like condensation domain
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                          • AGH68919.1
                          • mcl34
                          		 43295 - 44236 (-) SARP-like transcriptional regulator
                          • Regulation
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                          • AGH68920.1
                          • mcl35
                          		 44736 - 45551 (+) SARP-like transcriptional regulator
                          • Regulation
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                          • AGH68921.1
                          • mcl36
                          		 45645 - 46487 (-) SARP-like transcriptional regulator
                          • Regulation
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                          • AGH68922.1
                          • mcl37
                          		 47593 - 48585 (+) AfsA-like gamma-butyrolactone synthase
                          • Regulation
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                          • AGH68923.1
                          • mcl38
                          		 48730 - 49494 (+) oxidoreductase
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                            • AGH68924.1
                            • mcl39
                            		 51650 - 53245 (+) MFS-like transporter
                            • Transport
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                            • AGH68925.1
                            • mcl40
                            		 54769 - 56364 (+) vanadium-dependent chloroperoxidase
                            • Tailoring
                            • Other in vivo study
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                            • AGH68926.1
                            • mcl41
                            		 56801 - 57523 (+) thioredoxin-like oxidoreductase
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                              Biosynthesis information

                              Biosynthetic modules

                              Name
                              1
                              Type
                              pks-trans-at
                              Genes
                              mcl17
                              Substrates
                              Integrated Monomers
                              Domains
                              ketosynthase
                              Annotation changelog

                              Entry version: 4

                              Date
                              Changes
                              Submitters
                              Reviewers
                              2022-10-07
                              Update chemical activity to schema version 2.11
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                              Entry version: 3

                              Date
                              Changes
                              Submitters
                              Reviewers
                              2022-09-15
                              Corrected polyketide synthase gene entries
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                              2022-09-15
                              Removed ketoreductase stereochemistry annotation from modules without ketoreductases
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                              2022-09-15
                              Updated bioactivity data
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                              Entry version: 2

                              Date
                              Changes
                              Submitters
                              Reviewers
                              2019-10-16
                              Migrated from v1.4
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                              2019-10-16
                              Updated compound(s) information (NPAtlas curation)
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                              Entry version: 1

                              Date
                              Changes
                              Submitters
                              Reviewers
                              2015-06-12
                              Submitted
                              • (ID: MQCAQSV425YQGLSERITRLP7K)
                              • (ID: 7PXJSJT7IBMNIYLGG3GUM5VC)
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                              Detailed domain annotation
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