Genomic loci for this biosynthetic pathway

Cluster Type From To
The following clusters are from record BGC0001092.1:
Cluster 1NRP / Polyketide142785
The following clusters are from record BGC0001092.2:
Cluster 2NRP / Polyketide129948

BGC0001092, diaphorin biosynthetic gene cluster from Candidatus Profftella. Locus 1. Full MIBiG entry.

Chemical compounds

Compound: diaphorin
SMILES string: Copy to clipboard
Molecular formula: C22H39NO9
Exact molecular mass: 484.2517 Da ([M+H]+)
Molecular activity: Cytotoxic

Class-specific details

Biosynthetic class(es):
NRP / Polyketide
Other (linear)
Thioesterase type:
None
Release / cyclization type:
Other

Nonribosomal peptide synthetases:
dipT (AGS06887.1)
Module 5
A specificity: Glycine
Evidence for specificity: Sequence-based prediction
C domain subtype: LCL
Polyketide subclass:
Other (linear)
Polyketide synthase subclass:
Trans-AT type I
Starter unit:
Acetyl-CoA
AGS06838.1 AGS06886.1
Thioesterase type:
Type I
Release / cyclization type:
Unknown

Modular polyketide synthases:
dipP (AGS06823.1)
Module 0
AT specificity: Acetyl-CoA
Evidence for specificity: Sequence-based prediction
Scaffold-modifying domain: None
Module 1
KR stereochemistry: Unknown
Scaffold-modifying domain: Methylation
Module 2
Scaffold-modifying domain: Other
Module 3
KR stereochemistry: Unknown
Scaffold-modifying domain: Other

dipT (AGS06887.1)
Module 10
Scaffold-modifying domain: None
Module 13
KR stereochemistry: Unknown
Scaffold-modifying domain: None
Module 4
Scaffold-modifying domain: None
Module is non-elongating
Evidence for non-elongation: Unknown
Module 6
KR stereochemistry: Unknown
Scaffold-modifying domain: None
Module 7
KR stereochemistry: Unknown
Scaffold-modifying domain: Methylation
Module 8
KR stereochemistry: Unknown
Scaffold-modifying domain: Pyran synthase
Module 9
KR stereochemistry: Unknown
Scaffold-modifying domain: None

dipO (AGS06824.1)
Module 10
Scaffold-modifying domain: None
Module 11
KR stereochemistry: Unknown
Scaffold-modifying domain: None
Module 12
Scaffold-modifying domain: None

Gene cluster description

diaphorin (BGC0001092). Gene Cluster 1. Biosynthetic class = NRP/Polyketide. GenBank CP003468, positions 131663-174447. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Domain annotation

Homologous known gene clusters

General MIBiG information on this cluster

Complete gene cluster sequence?complete
Evidence for cluster-compound connection:Sequence-based prediction
MIxS-compliance:Unknown
Contact for this cluster:Eric J. N. Helfrich (Institute of Microbiology, Eidgenoessische Technische Hochschule (ETH) Zurich, Piel lab)

Literature references

1. Nakabachi A et al. (2013) Defensive bacteriome symbiont with a drastically reduced genome. Curr Biol 23(15):1478-84. doi: 10.1016/j.cub.2013.06.027. Epub 2013

BGC0001092, diaphorin biosynthetic gene cluster from Candidatus Profftella. Locus 2. Full MIBiG entry.

Chemical compounds

Compound: diaphorin
SMILES string: Copy to clipboard
Molecular formula: C22H39NO9
Exact molecular mass: 484.2517 Da ([M+H]+)
Molecular activity: Cytotoxic

Class-specific details

Biosynthetic class(es):
NRP / Polyketide
Other (linear)
Thioesterase type:
None
Release / cyclization type:
Other

Nonribosomal peptide synthetases:
dipT (AGS06887.1)
Module 5
A specificity: Glycine
Evidence for specificity: Sequence-based prediction
C domain subtype: LCL
Polyketide subclass:
Other (linear)
Polyketide synthase subclass:
Trans-AT type I
Starter unit:
Acetyl-CoA
AGS06838.1 AGS06886.1
Thioesterase type:
Type I
Release / cyclization type:
Unknown

Modular polyketide synthases:
dipP (AGS06823.1)
Module 0
AT specificity: Acetyl-CoA
Evidence for specificity: Sequence-based prediction
Module 1
KR stereochemistry: Unknown
Scaffold-modifying domain: Methylation
Module 2
Scaffold-modifying domain: Other
Module 3
KR stereochemistry: Unknown
Scaffold-modifying domain: Other

dipT (AGS06887.1)
Module 10
Module 13
KR stereochemistry: Unknown
Module 4
Module is non-elongating
Evidence for non-elongation: Unknown
Module 6
KR stereochemistry: Unknown
Module 7
KR stereochemistry: Unknown
Scaffold-modifying domain: Methylation
Module 8
KR stereochemistry: Unknown
Scaffold-modifying domain: Pyran synthase
Module 9
KR stereochemistry: Unknown

dipO (AGS06824.1)
Module 10
Module 11
KR stereochemistry: Unknown
Module 12

Gene cluster description

diaphorin (BGC0001092). Gene Cluster 2. Biosynthetic class = NRP/Polyketide. GenBank CP003468, positions 131663-174447. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Domain annotation

General MIBiG information on this cluster

Complete gene cluster sequence?complete
Evidence for cluster-compound connection:Sequence-based prediction
MIxS-compliance:Unknown
Contact for this cluster:Eric J. N. Helfrich (Institute of Microbiology, Eidgenoessische Technische Hochschule (ETH) Zurich, Piel lab)

Literature references

1. Nakabachi A et al. (2013) Defensive bacteriome symbiont with a drastically reduced genome. Curr Biol 23(15):1478-84. doi: 10.1016/j.cub.2013.06.027. Epub 2013